野生甜瓜‘云甜-930’抗白粉病主基因+多基因遗传分析

doi:10.3864/j.issn.0578-1752.2011.07.015

摘要/Abstract

摘要： 【目的】研究野生甜瓜‘云甜-930’对白粉病的抗性遗传规律，以充分合理利用抗源，为抗病基因的选择及甜瓜抗白粉病育种提供依据。【方法】采用植物数量性状主基因+多基因混合遗传模型多世代联合分析方法，对野生甜瓜‘云甜-930’(P1)与栽培甜瓜‘华莱士’(P2)杂交组合的P1、P2、F1、B1、B2和F26个世代群体抗白粉病特性进行分析。【结果】野生甜瓜‘云甜-930’对白粉病的抗性遗传符合两对加性-显性-上位性主基因+加性-显性-上位性多基因混合遗传模型(E-0)控制。在2008年大棚中，该组合B1、B2和F2的主基因遗传率分别为73.31%、69.15%和97.61%，多基因遗传率分别为18.83%、25.86%和0，环境方差占总表型方差的2.39%—7.86%；在2009年温室中，该组合B1、B2和F2的主基因遗传率分别为62.98%、58.58%和90.89%，多基因遗传率分别为28.93%、31.47%和3.22%，环境方差占总表型方差的5.89%—9.94%。【结论】野生甜瓜种质资源‘云甜-930’的抗白粉病遗传规律受两对加性-显性-上位性主基因+加性-显性-上位性多基因控制，同时还受到环境变异的影响。在抗白粉病育种中，F2主基因选择效率最高。

关键词: 野生甜瓜‘云甜-930&rsquo, 白粉病抗性 , P. xanthii , 生理小种 , 遗传规律

Abstract: 【Objective】 The inheritance law of powdery mildew resistance in wild melon material ‘Yuntian-930’ was studied for utilizing this resistance material and providing a theoretical basis for selecting resistance gene in melon breeding. 【Method】 Six populations (P1, P2, F1, B1, B2 and F2) derived from the cross of ‘Yuntian-930’× ‘Hualaishi’ with the highest resistant germplasm ‘Yuntian-930’ and the highest susceptible germplasm ‘Hualaishi’ were used to study the inheritance of powdery mildew resistance by the mixed major-gene plus polygene inheritance mode with joint analysis method of multiple generations. 【Result】The result showed that the inheritance of powdery mildew resistance of wild melon‘Yuntian-930’fitted two pairs of additive-dominance-epitasis major genes plus additive-dominant-epitasis polygene model (E-0 model). In 2008, the major genes heritability of B1, B2 and F2 were estimated to be 73.31%, 69.15% and 97.61%, respectively, the polygene heritability to be 18.83%, 25.86% and 0, and the ratios of the environmental variance to phenotype variance were 2.39%-7.86% in the plastic greenhouse. In 2009, the major genes heritability of B1, B2 and F2 were estimated to be 62.98%, 58.58% and 90.89% respectively, the polygene heritability to be 28.93%, 31.47% and 3.22%, and the ratios of the environmental variance to phenotype variance were 5.89%-9.94% in the greenhouse. 【Conclusion】 The powdery mildew resistance of wild melon ‘Yuntian-930’ was controlled by two pairs of additive-dominance-epitasis major genes plus additive-dominant-epitasis polygene, and also affected by environment. In the resistance breeding program, the selection efficiency to major gene of the F2 is the highest.

Key words: wild melon ‘Yuntian-930&rsquo, powdery mildew resistance , P. xanthii , race , inheritance

咸丰;张勇;马建祥;张显;杨建强. 野生甜瓜‘云甜-930’抗白粉病主基因+多基因遗传分析[J]. 中国农业科学, 2011, 44(7): 1425-1433.

XIAN Feng; ZHANG Yong; MA Jian-xiang; ZHANG Xian; YANG Jian-qiang. Genetic Analysis of Resistant to Powdery Mildew with Mixed Model of Major Gene Plus Polygene in Wild Melon Material ‘Yuntian-930’[J]. Scientia Agricultura Sinica, 2011, 44(7): 1425-1433.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2011.07.015

https://www.chinaagrisci.com/CN/Y2011/V44/I7/1425

参考文献

[1]Epinat C, Pitrat M, Bertrand F. Genetic analysis of resistance of   five melon lines to powdery mildews. Euphytica, 1993, 65 (2): 135-144.
[2]臧全宇. 网纹甜瓜白粉病抗性遗传研究[D]. 杭州: 浙江大学, 2007.
Zang Q Y. Studies on the inheritance of powdery mildew resistance in netted muskmelon [D]. Hangzhou: Zhejiang University, 2007. (in Chinese)
[3]王建设, 宋曙辉, 唐晓伟, 陈贵林. 甜瓜白粉病抗性基因的遗传与分子标记. 华北农学报, 2005, 20(1): 89-92.
Wang J S, Song S H, Tang X W, Chen G L. Genetics and molecular marker of the gene for disease to powdery mildew on Cucumis melo. L. Acta Agriculturae Boreali-Sinica, 2005, 20(1): 89-92. (in Chinese)
[4]Kenigsbuch D, Cohen Y. Inheritance and allelism of genes for resistance to races 1 and 2 of Sphaerotheca fuliginea in muskmelon. Plant Disease, 1992, 76(6): 626-629.
[5]程振家. 甜瓜(Cucumis mesa L. )白粉病抗性遗传机制及抗病基因AFLP分子标记研究[D]. 南京: 南京农业大学, 2006.
Cheng Z J. The genetic mechanisms of resistance to powdery mildews and ALFP marker linked to the disease resistance in melon[D]. Nanjing: Nanjing Agricultural University, 2006. (in Chinese)
[6]Teixeira A P M, Barreto F A D S, Camargo L E A. An AFLP marker linked to the Pm-1 gene that confers resistance to Podosphaera xanthii race 1 in Cucumis melo. Genetics and Molecular Biology, 2008, 31(2): 547-550.
[7]张海英, 苏　芳, 郭绍贵, 宫国义, 伊鸿平, 吴明珠, 许　勇. 甜瓜白粉病抗性基因Pm-2F的遗传特性及与其紧密连锁的特异片段. 园艺学报, 2008, 35(12): 1773-1780.
Zhang H Y, Su F, Guo S G, Gong G Y, Yi H P, Wu M Z, Xu Y. Genetic analysis and specific fragments linked to powdery mildew resistant gene Pm-2F in melon. Acta Horticulturae Sinica, 2008, 35(12): 1773-1780. (in Chinese)
[8]赵光伟. 甜瓜抗白粉病基因的SRAP分子标记及杭性反应中防御酶活性变化[D]. 北京: 中国农业科学院研究生院, 2009.
Zhao G W. Researches on SRAP molecular markers linked with resistant gene to powdery mildew in melon and changes of defense enzyme activity in resistance response[D]. Beijing: Chinese Academy of Agricultural Sciences, 2009. (in Chinese)
[9]Yuste-Lisbona F J, López-Sesé A I, Gómez-guillamón M L. Inheritance of resistance to races 1, 2 and 5 of powdery mildew in the melon TGR-1551. Plant Breeding, 2010, 129: 72-75.
[10]盖钧镒, 章元明, 王建康. 植物数量性状遗传体系. 北京: 科学出版社, 2003.
Gai J Y, Zhang Y M, Wang J K. Genetic System of Quantitative Traits in Plants. Beijing: Science Press, 2003. (in Chinese)
[11]朱世杨, 郭媛, 洪德林. 水稻种子抗老化遗传分析. 遗传, 2008, 30(2): 217-224.
Zhu S Y, Guo Y, Hong D L. Genetic analysis on aging-resistant in rice seed. Hereditas(Beijing), 2008, 30(2): 217-224. (in Chinese)
[12]侯北伟, 窦秉德, 章元明, 李生强, 杨晋彬, 刘福霞, 杜金昆, 孙其信. 小麦雌性育性的主基因+多基因混合遗传分析. 遗传, 2006, 28(12): 1567-1572.
Hou B W, Dou B D, Zhang Y M, Li S Q, Yang J B, Liu F X, Du J K, Sun Q X. The Mixed major gene plus polygenes inheritance for female fertility in wheat (Triticum aestivum L.). Hereditas(Beijing), 2006, 28(12): 1567-1572. (in Chinese)
[13]魏昕, 李丽华, 王娟, 樊庆琦, 兰海, 吴元奇, 荣廷昭, 潘光堂. 玉米丝裂病发生的数量遗传分析. 中国农业科学, 2008, 4l(8): 2235-2240.
Wei X, Li L H, Wang J, Fan Q Q, Lan H, Wu Y Q, Rong T Z, Pan G T. Quantitative genetic analysis of silk cut in maize (Zea mays L.). Scientia Agricultura Sinica, 2008, 41(8): 2235-2240. (in Chinese)
[14]詹秋文, 盖钧镒, 章元明, 孙祖东. 大豆对斜纹夜蛾幼虫抗性遗传的发展表达过程. 遗传学报, 2001, 28(10): 956-963.
Zhan Q W, Gai J Y, Zhang Y M, Sun Z D. Development and expression process of inheritance of resistance to cotton worm (Prodenia litura) in soybeans. Acta Genetica Sinica, 2001, 28(10): 956-963. (in Chinese)
[15]田露申, 牛应泽, 余青青, 郭世星, 柳丽. 甘蓝型油菜白花性状    的主基因+多基因遗传分析. 中国农业科学, 2009, 42(11): 3987-3995.
Tian L S, Niu Y Z, Yu Q Q, Guo S X, Liu L. Genetic analysis of white flower color with mixed model of major gene plus polygene in Brassica napus L. Scientia Agricultura Sinica, 2009, 42(11): 3987-3995. (in Chinese)
[16]刘二艳, 刘玉梅, 方智远, 杨丽梅, 庄　木, 张扬勇, 袁素霞, 孙继峰, 李占省, 孙培田. 青花菜花球‘荚叶’性状主基因+多基因遗传分析. 园艺学报, 2009, 36(11): 1611-1618.
Liu E Y, Liu Y M, Fang Z Y, Yang L M, Zhuang M, Zhang Y Y, Yuan S X, Sun J F, Li Z S, Sun P T. Genetic analysis of head-leaf traits using mixed major gene plus polygene inheritance model in Brassica oleracea L. var. italica Planch. Acta Horticulturae Sinica, 2009, 36(11): 1611-1618. (in Chinese)
[17]庞文龙, 刘富中, 陈钰辉, 连　勇. 茄子果色性状的遗传研究. 园艺学报, 2008, 35(7): 979-98.
Pang W L, Liu F Z, Chen Y H, Lian Y. Genetic study on fruit color traits of eggplant. Acta Horticulturae Sinica, 2008, 35(7): 979-986. (in Chinese)
[18]陈学军, 陈劲枫, 方　荣, 程志芳, 王述彬. 辣椒始花节位遗传研究. 园艺学报, 2006, 33(1): 152-154.
Chen X J, Chen J F, Fang R, Cheng Z F, Wang S B. Inheritance of the node for first flower in pepper (Capsicum annuum L.). Acta Horticulturae Sinica, 2006, 33(1): 152-154. (in Chinese)
[19]李纪锁, 沈火林, 石正强. 鲜食番茄果实中番茄红素含量的主基因-多基因混合遗传分析. 遗传, 2006, 28(4): 458-462.
Li J S, Shen H L, Shi Z Q. Analysis on the major gene and poly gene mixed in heritance of lycopene content in fresh consumptive tomato fruit. Hereditas (Beijing), 2006, 28(4): 458-462. (in Chinese)
[20]张　红, 王怀松, 贺超兴, 张志斌, 张　显, 伊鸿平, 吴明珠. 甜瓜糖酸性状的遗传研究. 园艺学报, 2009, 36(7): 989-996.
Zhang H, Wang H S, He C X, Zhang Z B, Zhang X, Yi H P, Wu M Z. Genetic study on sugar and sour traits of melon (Cucumis melo L.). Acta Horticulturae Sinica, 2009, 36(7): 989-996. (in Chinese)
[21]卓祖闯, 万恩梅, 张鲁刚, 张明科, 惠麦侠. 大白菜抽薹性状的主基因+多基因遗传分析. 西北植物学报, 2009, 29(5): 867-873.
Zhuo Z C, Wan E M, Zhang L G, Zhang M K, Hui M X. Major gene plus poly-gene inheritance analysis of bolting trait in heading Chinese cabbage. Acta Botanica Boreali-Occidentia Sinica, 2009, 29(5): 867-873. (in Chinese)
[22]陈学军, 陈劲枫. 辣椒株高遗传分析. 西北植物学报, 2006, 26(7): 1342-1345.
Chen X J, Chen J F. Genetic analysis of plant height in pepper (Capsicum annuum L.). Acta Botanica Boreali-Occidentia Sinica, 2006, 26(7): 1342-1345. (in Chinese)
[23]张素勤, 顾兴芳, 张圣平, 邹志荣. 黄瓜霜霉病抗性遗传分析. 西北植物学报, 2007, 27(12): 2416-2420.
Zhang S Q, Gu X F, Zhang S P, Zou Z R. Inheritance of       downy mildew resistance in cucumber (Cucumis sativus L.). Acta Botanica Boreali-Occidentia Sinica, 2007, 27(12): 1342-1345. (in Chinese)
[24]咸丰, 张勇, 马建祥, 张显, 杨建强, 许勇. 陕西关中地区瓜类白粉病菌生理小种的鉴定. 西北农林科技大学学报: 自然科学版, 2010, 38(10): 115-120.
Xian F, Zhang Y, Ma J X, Zhang X, Yang J Q, Xu Y. Identification of physiological races of cucurbits powdery mildew in Guanzhong areas, Shaanxi Province. Journal of Northwest A&F University: Natural Science Edition, 2010, 38(10): 115-120. (in Chinese)
[25]王娟, 邓建新, 宫国义, 郭绍贵, 王倩, 许勇. 甜瓜抗白粉病育种研究进展. 中国瓜菜, 2006(1): 33-36.
Wang J, Deng J X, Gong G Y, Guo S G, Wang Q, Xu Y. Breeding advances in powdery mildew of melon. China Cucurbits and Vegetables, 2006(1): 33-36. (in Chinese)
[26]王建设, 唐晓伟, 孟淑春, 宋曙辉, 陈贵林, 孙振英, 李　梅. 甜瓜白粉病抗源鉴定与抗性遗传分析. 华北农学报, 2002, 17(3): 124-128.
Wang J S, Tang X W, Meng S C, Song S H, Chen G L, Sun Z Y, Li M. Identification and preliminary genetic analysis of resistance to powdery mildew on melon. Acta Agriculturae Boreali-Sinica, 2002, 17(3): 124-128. (in Chinese)
[27]马鸿艳, 祖元刚, 栾非时. 甜瓜种质资源苗期抗白粉病鉴定. 东北农业大学学报, 2009, 40(12): 18-23.
Ma H Y, Zu Y G, Luan F S. Identification of melon germplasm resistance to powdery mildew in seedling stage. Journal of Northeast Agricultural University, 2009, 40(12): 18-23. (in Chinese)