关键词: 固相微萃取')">固相微萃取, 草莓, 气-质联用, 香气成分, 自动质谱解卷积鉴定系统

Abstract:

【Objective】 In this paper the extraction of strawberry aroma compounds with four coating materials of solid-phase micro-extraction (SPME) fiber and the automatic identification method for gas chromatography/mass spectrometry (GC/MS) data were researched. The aim of this experiment was to summarize a simple, accurate method for quality breeding and processing of strawberry. 【Method】 The aroma compounds in strawberry were detected by SPME-GC/MS- the automatic mass spectral deconvolution and identification system (AMDIS). 【Result】 A total of 97 compounds were identified from the sample extracts. The major constituents were esters, alcohols, ketones and terpenes, with the proportions of 42.3%, 13.4%, 10.3% and 6.2%, respectively. There are at least eight kinds of characteristic aroma components in strawberry. The most important aroma compounds were ethyl butanoate, ethyl hexanoate, linalool, furaneol, methyl butanoate, methyl hexanoate, 2-heptanone and nerolidol according to their aroma values. The 50/30 μm DVB/CAR/ PDMS fiber showed high sensitivity and selectivity for the determination of aroma compounds. The rest sequence was 100 μm PDMS,85 μm PA and 7 μm PDMS fibers in turn. Four SPME fibers had the similar extraction effect to ester. 50/30 μm DVB/CAR/PDMS fiber had significantly higher extraction effect than other fibers to ketones, terpenes, alcohols and aldehydes. 【Conclusion】 50/30 μm DVB /CAR /PDMS fiber is suitable for the extraction of aroma compounds of strawberry. The AMDIS is sensitive, accurate and rapid. It is worth to be popularized and applied.

Key words: solid phase micro-extraction ( SPME)')">solid phase micro-extraction ( SPME), strawberry, GC/MS, aroma compounds, AMDIS