关键词: 宁强矮马, 生长激素基因, 序列分析, 遗传变异

Abstract:

【Objective】 In order to provide a scientific basis for the origin and dwarfish mechanism of Ningqiang pony, the sequence of GH of different animals and some China horse breeds were studied. 【Method】 The PCR amplification, sequencing and DNAMAN software were used. 【Result】 There were 29 genetic variation, 11 loci were found in the coding region. Taking the AA sequence of GH of Mongolia horse as standard, there was 1 amino acid variation in Ningqiang pony. Through clustering analysis based on the sequence of mRNA of GH, the homology was 100%, 99.8%, 99.7%, between Ningqiang and Debao 67, Baise and Mongolia. 【Conclusion】 The homology level of GH was higher among Equus caballus, Sus scrofa and dog in present study, and the mutation of G→A affects the growth of Ningqiang pony.

Key words: Ningqiang pony, growth hormone gene, sequence analysis, genetic variation