关键词: 富贵竹, 杆状DNA病毒, 分子鉴定

Abstract: 【Objective】 In order to identify badnavirus in Dracaena sanderiana with typical mottle symptom from Hubei of China, and to compare the genome molecular variation between isolates of Dracaena sanderiana badnaviruses, the complete genome sequence of Dracaena sanderiana badnavirus Hubei isolate was obtained. 【Method】 The complete genome sequence was got through subsection cloning and sequencing, and the sequences were analyzed with BLAST and other softwares. 【Result】 The complete circular genome nucleotide sequence comprised 7 522bp, which contained 7 open reading frames （ORFs） on the plus-strand that potentially encoded proteins of 17.58 kD, 14.93 kD, 214.77 kD, 11.86 kD, 11.31 kD, 16.12 kD and 11.00 kD. The genome organization was highly similar with the newly reported badnavirus Dracaena mottle virus （DrMV）. The size of Hubei isolate was 9 nucleotides shorter than DrMV. They shared 99.7% identities over the entire nucleotide sequences, and 99.3%, 100%, 99.2% over the presumed amino acid sequences of ORFs 1-3, respectively. 【Conclusion】 The genome organization and the comparison analysis of sequences between the genomes of badnaviruses revealed that Dracaena sanderiana with typical mottle symptom from Hubei of China contained badnavirus, and Dracaena sanderiana badnavirus Hubei isolate and DrMV belonged to the same species, and the genome molecular variation between isolates of Dracaena sanderiana badnavirus was very small, which was much different from previous results that significant molecular variation existed in badnavirus isolates from different plants.

Key words: Dracaena sanderiana, badnavirus, molecular identification