关键词: 菠萝, 菠萝杆状 DNA 病毒, 杆状DNA病毒属, 全序列

Abstract:

【Objective】 Pineapple bacilliform comosus virus (PBCoV) was identified on pineapple in Zhanjiang district, Guangdong province, China, and the complete genomic sequence of PBCoV was analyzed and compared in the molecular variation among Badnaviruses. 【Method】 The specific primers of PBCoV were designed, and the viral partial and whole genomic sequences were, respectively, amplified with regular or inverse PCRs and analyzed by proper bioinformatics softwares. 【Result】 The complete circular genomic nucleotide sequence of PBCoV comprised 7 543 bp, which contained 3 ORFs on the plus-strand that potentially encoded proteins of 20.4, 14.0 and 217.6 kD, respectively. The nucleotide sequences of PBCoV shared 87%-97% identities with the partial sequences of PBCoV from Australia in GenBank. The amino acid sequence encoded by the viral ORF3 shared less than 50.8% identities with that of any known Badnaviruses. Evidence that the PBCoV sequence is integrated in the pineapple genome was presented and discussed. 【Conclusion】 The results indicated that PBCoV exists on pineapple in Zhangjiang district, Guangdong province, China. The virus belongs to Badnavirus, which shares the most identities in nucleotide level with Banana streak virus -Mys isolate. This is first to report the complete genomic sequence and structure of PBCoV.

Key words: pineapple, Pineapple bacilliform comosus virus, Badnavirus, complete sequence