关键词: 小麦条锈病, N.Strampelli, 抗病基因, 单体分析, 分子标记

Abstract:

【Objective】 N. Strampelli is a very important source of durable resistant material. Inheritance, location and molecular mapping of stripe rust resistance genes in N. Strampelli is important for revealing the resistance to stripe rust and developing durable cultivars. 【Method】 To confirm the resistant genes, N. Strampelli was crossed with susceptible cultivar MingXian169, Chinese spring and then backcrossed with them. The gene’s chromosome location was determined by the monosomic analysis of Chinese spring, which were then backcrossed among them. The microscope observation of F1 was performed to assess whether they were monosomic materials. To test molecular markers for N. Strampelli, 208 SSR pairs of primers, the PCR amplification, non-denatured PAGE and silver staining are used for detecting polymorphic bands. 【Result】 The resistance to SU-4 was dependently or overlappedly controlled by two recessive genes. The resistance to CYR31 was overlappedly controlled by two recessive genes. There was one recessive resistant gene controlled when tested with CYR29-mut3. It was temporarily named YrN.S. Xgwm499, Xwmc415 and Xwmc537 were located on chromorome 5BL. The map distance between Xgwm499, Xwmc415 and Xwmc537 were 7.6 cM, 5.4 cM and 10.7 cM, respectively. 【Conclusion】 The result indicated that YrN.S is a novel resistant gene which is different from other stripe rust resistance genes.

Key words: stripe rust, N.Strampelli, resistant gene, monosomic analysis, molecular mapping