|
Special Issue:
植物细菌真菌合辑Plant Bacteria/Fungus
|
|
|
|
| Bacterial extracts and bioformulates as a promising control of fruit body rot and root rot in avocado cv. Hass |
| David GRANADA1, Lorena LÓPEZ-LUJAN1, 2, Sara RAMÍREZ-RESTREPO1,2, Juan MORALES3, Carlos PELÁEZ-JARAMILLO4, Galdino ANDRADE5, Juan Carlos BEDOYA-PÉREZ2 |
1 Phytosanitary and Biological Control Unit, Corporation for Biological Research, Medellin 050034, Colombia
2 Faculty of Health Sciences, Institution University Colegio Mayor de Antioquia, Medellin 050034, Colombia
3 Department of Agronomic Sciences, National University of Colombia, Medellin 050034, Colombia
4 Faculty of Exact Sciences, University of Antioquia, Medellin 050010, Colombia
5 Department of Microbiology, Londrina State University, Londrina 86051-990, Brazil |
|
|
|
|
Abstract At least 20–40% of annual losses of avocado crops are caused by pathogenic fungi. The chemical treatments of these diseases are inefficient, cause environmental pollution and are increasingly restricted by international laws. This work aimed to assess the biocontrol capacity of a bacterial extract to protect avocado fruits and plants from pathogen infections. Extracts from the bacterial isolate Serratia sp. ARP5.1 were obtained from liquid fermentations in a biorreactor. A body rot postharvest infection model with Colletotrichum gloeosporioides on fruits was developed. Moreover, packaging conditions were simulated using the bacterial extract and the commercial fungicide prochloraz as a positive control. Additionally, seedlings infections with Phytophthora cinnamomi were performed on two types of avocado (West Indian race and cv. Hass). The Area Under Disease Progress Curve (AUDPC) was recorded using the bacterial extract and a commercial product with fosetyl-aluminium as treatments. The bacterial extract significantly reduced infections by C. gloeosporioides on injured avocado fruits at 31.1 µg mL–1. Intact fruits were also protected against body rot infections at the same concentration and showed no significant differences with the commercial fungicide. On the other hand, AUDPC in the seedlings was significantly reduced with the extract treatment at 3 µg mL–1 compared to the control. However, a possible phytotoxicity effect of the extract was evidenced in the seedlings and confirmed by pathogen recovery and tests on Raphanus sativus seedlings. Finally, formulations of the extracts (emulsion and emulsifiable concentrate) were prepared, and bioactive stability was assessed for 8 wk. The emulsion formulates demonstrated very stable bioactivity against P. cinnamomi. The extract and the emulsion formulate showed promising results for the control of avocado pathogens. New bioproducts based on this type of active principles could be developed for the benefit of avocado industry.
|
|
Received: 28 February 2019
Accepted:
|
| Fund: The authors give thanks to the Faculty of Health Sciences from the Institution University Colegio Mayor de Antioquia and the General System of Royalties from Antioquia, Colombia, for funding this project, also to the National University of Colombia and the Doctoral Fellowship Program (567) from Colciencias. |
|
Corresponding Authors:
Correspondence Juan Carlos Bedoya-Pérez, Tel: +57-4-4445611, E-mail: juan.bedoya@colmayor.edu.co
|
Cite this article:
David GRANADA, Lorena LóPEZ-LUJAN, Sara RAMíREZ-RESTREPO, Juan MORALES, Carlos PELáEZ-JARAMILLO, Galdino ANDRADE, Juan Carlos BEDOYA-PéREZ.
2020.
Bacterial extracts and bioformulates as a promising control of fruit body rot and root rot in avocado cv. Hass. Journal of Integrative Agriculture, 19(3): 748-758.
|
Anderson A J, Kim Y C. 2018. Biopesticides produced by plant-probiotic Pseudomonas chlororaphis isolates. Crop Protection, 105, 62–69.
Andrade-Hoyos P, Espíndola-Barquera M C, Molina-Gayosso E, De León C, Alvarado-Rosales D, López-Jiménez A. 2015. Mecanismos de defensa en portainjertos de aguacate resistentes a Phytophthora cinnamomi Rands. VIII Congreso Mundial de La Palta, 6, 141–147. (in Spanish)
Araújo R G, Rodriguez-Jasso R M, Ruiz H A, Pintado M M, Aguilar C N. 2018. Avocado by-products: Nutritional and functional properties. Trends in Food Science & Technology, 80, 51–60.
Arias C, Moors E H M. 2018. Reducing post-harvest food losses through innovative collaboration: Insights from the Colombian and Mexican avocado supply chains. Journal of Cleaner Production, 199, 1020–1034.
Bandiera M, Mosca G, Vamerali T. 2009. Humic acids affect root characteristics of fodder radish (Raphanus sativus L. var. oleiformis Pers.) in metal-polluted wastes. Desalination, 246, 78–91.
Bill M, Sivakumar D, Korsten L, Thompson A K. 2014. The efficacy of combined application of edible coatings and thyme oil in inducing resistance components in avocado (Persea americana Mill.) against anthracnose during post-harvest storage. Crop Protection, 64, 159–167.
Bittner R J, Mila A L. 2017. Efficacy and timing of application of oxathiapiprolin against black shank of flue-cured tobacco. Crop Protection, 93(Suppl. C), 9–18.
Campos-Martínez A, Velázquez-del-Valle M G, Flores-Moctezuma H E, Suárez-Rodríguez R, Ramírez-Trujillo J A, Hernández-Lauzardo A N. 2016. Antagonistic yeasts with potential to control Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. and Colletotrichum acutatum J.H. Simmonds on avocado fruits. Crop Protection, 89, 101–104.
Costa J L, Menge J A, Casale W L. 1996. Investigations on some of the mechanisms by which bioenhanced mulches can suppress Phytophthora root rot of avocado. Microbiological Research, 151, 183–192.
Cui H, Zhang X, Zhou H, Zhao C, Xiao Z, Lin L, Li C. 2015. Antibacterial properties of nutmeg oil in pork and its possible mechanism. Journal of Food Safety, 35, 370–377.
Demoz B T, Korsten L. 2006. Bacillus subtilis attachment, colonization, and survival on avocado flowers and its mode of action on stem-end rot pathogens. Biological Control, 37, 68–74.
Engelbrech J, Van-Den-Berg N. 2013. Expression of defence-related genes against Phytophthora cinnamomi in five avocado rootstocks. South African Journal of Science, 109, 1–8.
Everett K, Owen S G, Cutting J G M. 2005. Testing efficacy of fungicides against postharvest pathogens of avocado (Persea americana cv. Hass). New Zealand Plant Protection, 58, 89–95.
Fischer I H, Moraes M F, Palharini M C, Fileti M S, Cruz J C, Firmino A C. 2018. Effect of conventional and alternative products on postharvest disease control in avocados. Revista Brasileira de Fruticultura, 40, e-408.
Glowacz M, Roets N, Sivakumar D. 2017. Control of anthracnose disease via increased activity of defence related enzymes in Hass avocado fruit treated with methyl jasmonate and methyl salicylate. Food Chemistry, 234, 163–167.
Granada-García S D, Rueda-Lorza A, Peláez C A. 2014. Antimicrobial activity of extracellular metabolites from antagonistic bacteria isolated from potato (Solanum phureja) crops. Summa Phytopathologica, 40, 212–220.
Guardado-Valdivia L, Tovar-Pérez E, Chacón-López A, López-García U, Gutiérrez-Martínez P, Stoll A, Aguilera S. 2018. Identification and characterization of a new Bacillus atrophaeus strain B5 as biocontrol agent of postharvest anthracnose disease in soursop (Annona muricata) and avocado (Persea americana). Microbiological Research, 210, 26–32.
Guevara-Avendaño E, Bejarano-Bolívar A A, Kiel-Martínez A L, Ramírez-Vázquez M, Méndez-Bravo A, Aguirre E, Sánchez-Rangel D, Guerrero-Analco J A, Eskalen A, Reverchon F. 2019. Avocado rhizobacteria emit volatile organic compounds with antifungal activity against Fusarium solani, Fusarium sp. associated with Kuroshio shot hole borer, and Colletotrichum gloeosporioides. Microbiological Research, 219, 74–83.
Gutierrez-Monsalve J A, Mosquera S, González-Jaramillo L M, Mira J J, Villegas-Escobar V. 2015. Effective control of black Sigatoka disease using a microbial fungicide based on Bacillus subtilis EA-CB0015 culture. Biological Control, 87, 39–46.
Hernández A F, Parrón T, Tsatsakis A M, Requena M, Alarcón R, López-Guarnido O. 2013. Toxic effects of pesticide mixtures at a molecular level: Their relevance to human health. Toxicology, 307(Suppl. C), 136–145.
Horsfall J G, Barratt R W. 1945. An improved grading system for measuring plant diseases. Phytopathology, 35, 655–655.
Hurtado-Fernández E, Fernández-Gutiérrez A, Carrasco-Pancorbo A. 2018. Avocado fruit - Persea americana. In: Rodrigues S, Oliveira E, Sousa E, eds., Exotic Fruits. Academic Press, Spain. pp. 37–48.
Khalid N, Shu G, Kobayashi I, Nakajima M, Barrow C J. 2017. Formulation and characterization of monodisperse O/W emulsions encapsulating astaxanthin extracts using microchannel emulsification: Insights of formulation and stability evaluation. Colloids and Surfaces (B: Biointerfaces), 157, 355–365.
Leal J M, Castaño J, Bolaños M M. 2014. Management of avocado (Persea americana LINNEO) root rot (Phytophthora cinnamomi RANDS). Revista U.D.C.A Actualidad & Divulgación Científica, 17, 105–114. (in Spanish)
Lopes L P, Oliveira A G, Beranger J P O, Góis C G, Vasconcellos F C, San-Martin J A B, Andrade G. 2012. Activity of extracellular compounds of Pseudomonas sp. against Xanthomonas axonopodis in vitro and bacterial leaf blight in eucalyptus. Tropical Plant Pathology, 37, 233–238.
Matilla M A, Stöckmann H, Leeper F J, Salmond G P C. 2012. Bacterial biosynthetic gene clusters encoding the anti-cancer haterumalide class of molecules: Biogenesis of the broad spectrum antifungal and anti-oomycete compound, oocydin A. Journal of Biological Chemistry, 287, 39125–39138.
Matusinsky P, Zouhar M, Pavela R, Novy P. 2015. Antifungal effect of five essential oils against important pathogenic fungi of cereals. Industrial Crops and Products, 67(Suppl. C), 208–215.
McLeod A, Masikane S L, Novela P, Ma J, Mohale P, Nyoni M, StanderM , Wessels J, Pieterse P. 2018. Quantification of root phosphite concentrations for evaluating the potential of foliar phosphonate sprays for the management of avocado root rot. Crop Protection, 103, 87–97.
Ocampo-Suarez I B, López Z, Calderón-Santoyo M, Ragazzo-Sánchez J A, Knauth P. 2016. Are biological control agents, isolated from tropical fruits, harmless to potential consumers? Food and Chemical Toxicology, 109, 1055–1062.
Pérez-Jiménez R M. 2008. Significant avocado diseases caused by fungi and oomycetes. The European Journal of Plant Science and Biotechnology, 2, 1–24.
R Core Team. 2018. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. [2018-02-15]. https://www.R-project.org/
Ramírez-Gil J G, Castañeda-Sánchez D A, Morales-Osorio J G. 2014. Estudios etiológicos de la marchitez del aguacate en Antioquia-Colombia. Revista Ceres, 61, 50–61. (in Spanish)
Ramírez-Gil J G, Castañeda-Sánchez D A, Morales-Osorio J G. 2017a. Production of avocado trees infected with Phytophthora cinnamomi under different management regimes. Plant Pathology, 66, 623–632.
Ramírez-Gil J G, Gilchrist-Ramelli E, Morales-Osorio J G. 2017b. Economic impact of the avocado (cv. Hass) wilt disease complex in Antioquia, Colombia, crops under different technological management levels. Crop Protection, 101, 103–115.
Rodrigues S, Cantuarias-Avilés T, Bremer-Neto H, Alves-Mourão F. Bordignon-Medina R. 2016. Management of root rot in avocado trees. Revista Brasileira de Fruticultura, 38, e-175.
Ruano-Rosa D, Arjona-Girona I, López-Herrera C J. 2018. Integrated control of avocado white root rot combining low concentrations of fluazinam and Trichoderma spp. Crop Protection, 112, 363–370.
Sarkhosh A, Vargas A I, Schaffer B, Palmateer A J, Lopez P, Soleymani A, Farzaneh M. 2017. Postharvest management of anthracnose in avocado (Persea americana Mill.) fruit with plant-extracted oils. Food Packaging and Shelf Life, 12, 16–22.
Scheepers S, Jooste A, Alemu Z. 2007. Quantifying the impact of phytosanitry standards with spesific reference to MRLs on the trade flow of South African avocados to the EU. Agrekon, 46, 260–273.
Schmidt W, Redshaw C H. 2015. Evaluation of biological endpoints in crop plants after exposure to non-steroidal anti-inflammatory drugs (NSAIDs): Implications for phytotoxicological assessment of novel contaminants. Ecotoxicology and Environmental Safety, 112(Suppl. C), 212–222.
Shaner G, Finney R E. 1977. The effect of nitrogen fertilization on the expression of slow-mildewing resistance in knox wheat. Phytopathology, 77, 1051–1056.
Sena K, Crocker E, Vincelli P, Barton C. 2018. Phytophthora cinnamomi as a driver of forest change: Implications for conservation and management. Forest Ecology and Management, 409, 799–807.
Singh D, Sharma R R. 2018. Chapter 1 - Postharvest diseases of fruits and vegetables and their management. In: Siddiqui M W, ed., Postharvest Disinfection of Fruits and Vegetables. Academic Press, Cambridge, EE.UU. pp. 1–52.
Smith J, Korsten L. 1996. Microbes associated with the avocado flower and fruit: The good, the bad and the ugly. South African Avocado Growers’ Association Yearbook, 19, 39–40.
Tan C X. 2019. Virgin avocado oil: An emerging source of functional fruit oil. Journal of Functional Foods, 54, 381–392.
Velivelli S L S, De-Vos P, Kromann P, Declerck S, Prestwich B D. 2014. Biological control agents: From field to market, problems, and challenges. Trends in Biotechnology, 32, 493–496.
Yahia E M, Woolf A B. 2011. Avocado (Persea americana Mill.). In: Yahia E, ed., Postharvest Biology and Technology of Tropical and Subtropical Fruits. Woodhead Publishing Series in Food Science, Technology and Nutrition, Woodhead Publishing Limited, Cambridge, UK. p. 534.
You M P, Sivasithamparam K. 1995. Changes in microbial populations of an avocado plantation mulch suppressive of Phytophthora cinnamomi. Applied Soil Ecology, 2, 33–43.
Zhang H, Mahunu G K, Castoria R, Apaliy M T, Yang Q. 2017. Augmentation of biocontrol agents with physical methods against postharvest diseases of fruits and vegetables. Trends in Food Science & Technology, 69, 36–45. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
