? Development of a stable SCAR marker for rapid identification of <em>Ganoderma lucidum</em> Hunong 5 cultivar using DNA pooling method and inter-simple sequence repeat markers
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    2018, Vol. 17 Issue (01): 130-138     DOI: 10.1016/S2095-3119(17)61825-2
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Development of a stable SCAR marker for rapid identification of Ganoderma lucidum Hunong 5 cultivar using DNA pooling method and inter-simple sequence repeat markers
CHAO Wen-zheng1, 2, TANG Chuan-hong2, 3, ZHANG Jing-song2, YU Ling1, Honda Yoichi3  
1 School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, P.R.China
2 Key Laboratory of Edible Fungus Resources and Utilization (South), Ministry of Agriculture/National Engineering Research Center of Edible Fungi/National R&D Center for Edible Fungi Processing/Key Laboratory of Agriculture Genetics and Breeding of Shanghai, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, P.R.China
3 Laboratory of Forest Biochemistry, Graduate School of Agriculture, Kyoto University, Kyoto 6068502, Japan
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Abstract The cultivar Ganoderma lucidum Hunong 5 was obtained using cross-breeding.  Hunong 5 has high commercial value due to its high polysaccharide and triterpene content.  This is the first report of using a DNA pooling method to develop a stable sequence characterized amplified region (SCAR) marker for rapid identification of the G. lucidum Hunong 5 cultivar.  The SCAR marker was developed by first generating and sequencing a distinctive inter simple sequence repeat (ISSR) fragment (882 bp) from G. lucidum Hunong 5 cultivar.  A stable SCAR primer pair GLH5F/GLH5R were obtained to identify the cultivar and the SCAR marker is a DNA fragment of 773 bp.
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Key wordsDNA pooling     Ganoderma lucidum    Hunong 5 cultivar     ISSR marker     SCAR marker     
Received: 2017-04-06; Published: 2017-06-12
Fund:

This work was financially supported by the National Natural Science Foundation of China (31401933) and the Shanghai Municipal Committee of Agriculture, China (G2014070107).

Corresponding Authors: Correspondence TANG Chuan-hong,E-mail:tangchuanhong123@163.com;ZHANG Jing-song, Tel: +86-21-62200746,Fax:+86-21-62201530, E-mail: zhangjs888@163.com   
Cite this article:   
CHAO Wen-zheng, TANG Chuan-hong, ZHANG Jing-song, YU Ling, Honda Yoichi. Development of a stable SCAR marker for rapid identification of Ganoderma lucidum Hunong 5 cultivar using DNA pooling method and inter-simple sequence repeat markers[J]. Journal of Integrative Agriculture, 2018, 17(01): 130-138.
URL:  
http://www.chinaagrisci.com/Jwk_zgnykxen/EN/ 10.1016/S2095-3119(17)61825-2      or     http://www.chinaagrisci.com/Jwk_zgnykxen/EN/Y2018/V17/I01/130
 
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