6-Benzylaminopurine treatment maintains the quality of Chinese chive (Allium tuberosum Rottler ex Spreng.) by enhancing antioxidant enzyme activity
JIA Li-e1, LIU Sheng1, DUAN Xiao-ming1, ZHANG Chao1, WU Zhan-hui1, LIU Ming-chi1, GUO Shao-gui1, ZUO Jin-hua1, WANG Li-bin2
1 Key Laboratory of Urban Agriculture (North), Ministry of Agriculture/Key Laboratory of Vegetable Postharvest Processing, Ministry of Agriculture/Key Laboratory of Vegetable Postharvest Processing, Ministry of Agriculture/Beijing Key Laboratory of Fruits and Vegetable Storage and Processing, National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, P.R.China 2 College of Food Science and Technology, Yangzhou University, Yangzhou 225127, P.R.China
Abstract Chinese chive usually develops an off-flavor after a short storage time. To explore effective ways to maintain the postharvest quality of Chinese chive, the effect of exogenous application of 6-benzylaminopurine (6-BA) on postharvest quality and antioxidant activity of chive was evaluated, and the mechanism of the physiological responses of chive to 6-BA treatment was explored. Chives were sprayed for 10 min with 100, 300, or 500 mg L–1 6-BA or with alkaline solution as the control, then stored at (2±1)°C with a relative humidity (RH) of 80–85%. We found that 300 mg L–1 6-BA significantly delayed yellowing and chlorophyll degradation, maintained the total phenolic and flavonoid content, and improved the activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD). In conclusion, we identified exogenous application of 6-BA as an effective method for maintaining postharvest quality of Chinese chive. In addition, our finding that the activities of antioxidant enzymes increase in response to exogenous 6-BA provides new insights into the mechanism of cytokinin-based postharvest fresh-keeping.
This work was financially supported by the Innovation Capacity Building Projects of Beijing Academy of Agricultural and Forestry Sciences, China (KJCX20140205) and the Innovation Capacity Building Projects of Beijing Academy of Agricultural and Forestry Sciences, China (KJCX20140408).
About author: JIA Li-e, Mobile: +86-13301162561, E-mail: email@example.com;
Cite this article:
JIA Li-e, LIU Sheng, DUAN Xiao-ming, ZHANG Chao, WU Zhan-hui, LIU Ming-chi, GUO Shao-gui, ZUO Jin-hua, WANG Li-bin. 6-Benzylaminopurine treatment maintains the quality of Chinese chive (Allium tuberosum Rottler ex Spreng.) by enhancing antioxidant enzyme activity[J]. Journal of Integrative Agriculture,
2017, 16(09): 1968-1977.
An J S, Zhang M, Lu Q R, Zhang Z G. 2006. Effect of a pre-storage treatment with 6-benzylaminopurine and modified atmosphere packaging storage on the respiration and quality of green asparagus spears. Journal of Food Engineering, 77, 951-957.
AOAC (Association of Official Analytical Chemists). 2000. Official Methods of Analysis of the Association of the Official Analysis Chemists. 17th ed. AOAC, USA. p. 2000.
Ben-Yehoshua S, Rodov V, Bartz J, Brecht J. 2003. Transpiration and water stress.In: Postharvest Physiology and Pathology of Vegetables. Marcel Dekker, New York. pp. 111-159.
Borrel A, Carbonell L, Farràs R, Puig-Parcllada P, Tiburcio A F. 1997. Polyamines inhibit lipid peroxidation in senescing oat leaves. Physiologia Plantarum, 99, 385-390.
Bradford M M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254.
Cakmak I, Marschner H. 1992. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, aseorbate peroxidase, and glutathione reductase in bean leaves. Plant Physiology, 98, 1222-1227.
Cantwell M I, Thangaiah A. 2012. Acceptable cooling delays for selected warm season vegetables and melons. Acta Horticulture, 934, 77-84.
Carocho M, Ferreira I C F R. 2013. A review on antioxidants, prooxidants and related controversy: Natural and synthetic compounds, screening and analysis methodologies and future perspectives. Food and Chemical Toxicology, 51, 15-25.
Chen S Y, Zhao X H, Liu H Z, Li J C. 2014. Effect of GA and 6-BA treatments on antioxidative properties of fresh-cut broccoli florets. Food and Fermentation Industries, 40, 230-234. (in Chinese)
Costa M L, Civello P M, Chaves A R, Martínez G A. 2005. Effect of ethephon and 6-benzylaminopurine on chlorophyll degrading enzymes and a peroxidase-linked chlorophyll bleaching during post-harvest senescence of broccoli (Brassica oleracea L.) at 20°C. Postharvest Biology and Technology, 35, 191-199.
Downs C G, Somerfield S D, Davey M C. 1997. Cytokinin treatment delays senescence but not sucrose loss in harvested broccoli. Postharvest Biology and Technology, 11, 93-100.
Elstner E F. 2003. Oxygen activation and oxygen toxicity. Annual Review of Plant Physiology, 33, 73-96.
Fang Y Z, Zheng R L. 2002. Theory and Application of Free Radical Biology.Science Press, Beijing. pp. 56-78. (in Chinese)
Gao J X, Liu D, Gu R X, Hu H L, Li P X. 2015. 6-Benzyl aminopurine treatment delays the quality deterioration of Pakchoi (Brassica chinensis L.) during storage. Food Science, 36, 247-253. (in Chinese)
Garcia E, Barrett D M. 2002. Preservative treatments for fresh-cut fruits and vegetables.In: Fresh-Cut Fruits and Vegetables. CRC Press, Boca Raton, FL. pp. 267-304.
Gong X J. 2003. Studies on the fresh-keeping of day-lily flower. MSc thesis, Hunan Agricultural University, China. (in Chinese)
Guo Y Y, Zhang N, Zhu Y H, Yin Y B. 2008. Effects of gibberellin on the mineral elements content and preservation of broccoli flower head. Transactions of the Chinese Society of Agricultural Engineering, 24, 274-278. (in Chinese)
Hou J S, Li Z H, Wang J L. 2003. Effect of film packaging and cold storage on chives. China Vegetables, 1, 12-13. (in Chinese)
Imahori Y, Bai J H, Baldwin E. 2016. Antioxidative responses of ripe tomato fruit to postharvest chilling and heating treatments. Scientia Horticulturae, 198, 398-406.
Imahori Y, Suzuki Y, Kawagishi M, Ishimaru M, Ueda Y, Chachin K. 2007. Physiological responses and quality attributes of Chinese chive leaves exposed to CO2-enriched atmospheres. Postharvest Biology and Technology, 46, 160-166.
Imahori Y, Suzuki Y, Uemura K, Kishioka I, Fujiwara H, Ueda Y, Chachin K. 2004. Physiological and quality responses of Chinese chive leaves to low oxygen atmospheres. Postharvest Biology and Technology, 31, 295-303.
Inskeep W P, Bloom P R. 1985. Extinction coefficients of chlorophyll a and b in N,N-dimethylformamide and 80% acetone. Plant Physiology, 77, 483-485.
Ishii K, Okubo M. 1984. The keeping quality of Chinese chive (Allium tuberosum Rottler) by low temperature and seal packaging with polyethylene bag. Journalof theJapaneseSocietyforHorticulturalScience, 53, 87-95. (in Japanese)
Koukounaras A, Siomos A S, Sfakiotakis E.2010.Effects of 6-BA treatments on yellowing and quality of stored rocket (Eruca satita Mill.) leaves. Journal of Food Quality, 33, 768-779.
Li P X, Hu H L, Wang Y N. 2012. Effects of exogenous nitric oxide on lignification and anti-oxidation activity of postharvest green asparagus. Transactions of the Chinese Society of Agricultural Engineering, 28, 264-269. (in Chinese)
Lin J Y, Tang C Y. 2007. Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food Chemistry, 101, 140-147.
Lin Y J, Hao Y L. 2009. Physiological characteristics of plant senescence. Animal Husbandry Feed Science, 30, 8-10. (in Chinese)
Looney N E. 1979. Some effects of gibberellins A4+7 benzyladenine on fruit weight, shape, quality, Ca content, and storage behavior of ‘Spartan’ apple. Journal of the American Society for Horticultural Science, 104, 389-391.
Lu H, Gao J X, Hu H L, Li P X. 2016. Effect of 6-benzylaminopurine treatment on postharvest quality and reactive oxygen species metabolism of ipomoea aquatica. JournalofFoodScience, 37, 266-271. (in Chinese)
Lurie S, Fallik E, Handros A, Shapira R. 1997. The possible involvement of peroxidase in resistance to Botrytis cinerea in heat treated tomato fruit. Physiological and Molecular Plant Pathology, 50, 141-149.
Ma F W, Li J R, Ji A M. 1994. Effects of several fruit factors on weightlessness of kiwi during storage. Deciduous Fruit Trees, 26, 13-14.
Ma S J. 2012. Green leafy vegetables chlorophyll degradation during the aging process and control. China Science & Technology Panorama Magazine, 16, 49-50. (in Chinese)
Martino G D, Vizovitis K, Botondi R, Bellincontro A, Mencarelli F. 2006. 1-MCP controls ripening induced by impact injury on apricots by affecting SOD and POX activities. Postharvest Biology and Technology, 39, 38-47.
Polle A. 2001. Dissecting the superoxide dismutase-ascorbate-glutathione-pathway in chloroplasts by metabolic modeling. Computer simulations as a step towards flux analysis. Plant Physiology, 126, 445-462.
Rao P S, Kalva S, Yerramilli A, Mamidi S. 2011. Free radicals and tissue damage: Role of antioxidants. Free Radicals and Antioxidants, 1, 2-7.
Rushing J W. 1990. Cytokinins affect respiration, ethylene production, and chlorophyll retention of packaged broccoli florets.HortScience, 25, 88-90.
Shen W B, Ye M B, Xu L L, Zhang R X. 1997. Changes of ability of scavenging active oxygen during natural senescence of wheat flag leaves. Acta Botanica Sinica, 39, 634-640.
Siddiqui M W, Bhattacharjya A, Chakraborty I, Dhua R S. 2011. 6-Benzylaminopurine improves shelf life, organoleptic quality and health-promoting compounds of fresh-cut broccoli florets. Journal of Scientific and Industrial Research, 70, 461-465
Siddiqui M W, Dutta P, Dhua R S, Dey A. 2014. Changes in biochemical composition of mango in response to pre-harvest gibberellic acid spray. Agriculturae Conspectus Scientificus, 78, 331-335.
Surówka E, Karolewski P, Niewiadomska E, Libik M, Miszalski Z. 2007. Antioxidative response of Mesembryanthemum crystallinum plants to exogenous SO2 application.Plant Science,172, 76-84.
Tian M S, Downs C G, Lill R E, King G A. 1994. A role for ethylene in the yellowing of broccoli after harvest.Journal of the American Society for Horticultural Science,119, 276-281.
Toivonen P M A, Sweeney M. 1998. Differences in chlorophyll loss at 13°C for two broccoli (Brassica oleracea L.) cultivars associated with antioxidant enzyme activities. Journal of Agricultural and Food Chemistry, 46, 20-24.
Vinson J A, Hao Y, Su X, Zubik L. 1998. Phenol antioxidant quantity and quality in foods: Vegetables. Journal of Agricultural and Food Chemistry, 46, 3630-3634.
Wang Y S, Tian S P, Xu Y. 2005. Effects of high oxygen concentration on pro- and anti-oxidant enzymes in peach fruits during postharvest periods.Food Chemistry,91, 99-104.
Waterman P G, Mole S. 1994. Analysis of Phenolic Plant Metabolites. Blackwell Scientific Publications, Oxford.
Wu Y M, Hua X Z, Fang J X, Liu Y. 1991. Effects of storage temperature and gas composition on ethylene biosynthesis of apple. Plant Physiology Journal, 17, 169-176. (in Chinese)
Xu F, Yang Z F, Chen X H, Jin P, Wang X L, Zheng Y H. 2012. 6-Benzylaminopurine delays senescence and enhances health-promoting compounds of harvested broccoli.Journal of Agricultural and Food Chemistry, 60, 234-240.
Zhao Y Y, Shi P B, Yu X R, Chang X D, Huang Y X. 2011. Effects of 6-BA on the storage of green asparagus. Preservation and Processing, 11, 14-17. (in Chinese)
Zheng Y, Cao M, Shen L, Sheng J P. 2011. Effect of ultraviolet C irradiation on post-harvest quality and reactive oxygen speciesmetabolism-relatedenzymes in leeks. Food Science, 32, 307-311. (in Chinese)