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A stable and major QTL region on chromosome 2 conditions pod shape in cultivated peanut (Arachis hyopgaea L.)

ZHANG Sheng-zhong, HU Xiao-hui, WANG Fei-fei, CHU Ye, YANG Wei-qiang, XU Sheng, WANG Song, WU Lan-rong, YU Hao-liang, MIAO Hua-rong, FU Chun, CHEN Jing

2023, 22 (8): 2323-2334. DOI: 10.1016/j.jia.2023.02.005

Abstract （309） PDF in ScienceDirect

Peanut pod shape is a heritable trait which affects the market acceptance of in-shell peanut products. In order to determine the genetic control of pod shape, six component traits of pod shape (pod length, pod width, pod length/width ratio, pod roundness, beak degree and constriction degree) were measured using an image-based phenotyping method. A recombinant inbred line (RIL) population consisting of 181 lines was phenotyped across three environments. Continuous distributions and transgressive segregations were demonstrated in all measured traits and environments. Significant correlations were found among most component traits with broad-sense heritability ranging from 0.87 to 0.95. Quantitative trait locus (QTL) analysis yielded 26 additive QTLs explaining 3.79 to 52.37% phenotypic variations. A novel, stable and major QTL region conditioning multiple shape features was detected on chromosome 2, which spans a 10.81-Mb genomic region with 543 putative genes. Bioinformatics analysis revealed several candidate genes in this region. In addition, 73 pairs of epistatic interactions involving 92 loci were identified for six component traits explaining 0.94–6.45% phenotypic variations. These results provide new genetic loci to facilitate genomics-assisted breeding of peanut pod shape.

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Farmers’ precision pesticide technology adoption and its influencing factors: Evidence from apple production areas in China

YUE Meng, LI Wen-jing, JIN Shan, CHEN Jing, CHANG Qian, Glyn JONES, CAO Yi-ying, YANG Gui-jun, LI Zhen-hong, Lynn J. FREWER

2023, 22 (1): 292-305. DOI: 10.1016/j.jia.2022.11.002

Abstract （198） PDF in ScienceDirect

The research aimed to understand farmers’ willingness to adopt (WTA) and willingness to pay (WTP) for precision pesticide technologies and analyzed the determinants of farmers’ decision-making. We used a two-stage approach to consider farmers’ WTA and WTP for precision pesticide technologies. A survey of 545 apple farmers was administered in Bohai Bay and the Loess Plateau in China. The data were analyzed using the double-hurdle model. The results indicated that 78.72% of respondents were willing to apply precision pesticide technologies provided by service organizations such as cooperatives and dedicated enterprises, and 69.72% were willing to buy the equipment for using precision pesticide technologies. The results of the determinant analysis indicated that farmers’ perceived perceptions, farm scale, cooperative membership, access to digital information, and availability of financial services had significant and positive impacts on farmers’ WTA precision pesticide technologies. Cooperative membership, technical training, and adherence to environmental regulations increased farmers’ WTP for precision pesticide technologies. Moreover, nonlinear relationships between age, agricultural experience, and farmers’ WTA and WTP for precision pesticide technology services were found.

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Application of Chinese milk vetch affects rice yield and soil productivity in a subtropical double-rice cropping system

CHEN Jing-rui, QIN Wen-jing, CHEN Xiao-fen, CAO Wei-dong, QIAN Guo-min, LIU Jia, XU Chang-xu

2020, 19 (8): 2116-2126. DOI: 10.1016/S2095-3119(19)62858-3

Abstract （152） PDF in ScienceDirect

Green manure can be used as a substitute for chemical fertilizer without reducing rice yield. We studied the responses of soil fertility and rice yield to different combinations of Chinese milk vetch (CMV; Astragalus sinicus L.) and chemical fertilizer in a subtropical double-rice cropping system. Our goal is to reduce chemical fertilizer use and decrease environmental contamination. Compared with the recommended rate of chemical fertilizer (CF), both early- and late-rice yields in the two treatments supplied with 15 and 22.5 Mg CMV ha^–1 plus 60% CF (represented as 60A and 60B, respectively) showed no significant differences while the two treatments supplied with 30 and 37.5 Mg CMV ha^–1 plus 60% CF (represented as 60C and 60D, respectively) showed significantly higher values. The sustainable yield index (SYI) values in the 60C and 60D treatments with double-rice croppong system were significantly higher than those in other treatments (P<0.05). Early-rice yield showed a significant positive relationship with the Chinese milk vetch incorporation rate. The coefficients increased annually from 2009 to 2013 and then decreased in 2014. Soil organic matter increased over time by the end of the experiment in all of the treatment groups. Soil organic matter in 60A, 60B and 60C showed no significant difference compared with that in CF, while soil organic matter in 60D was significantly higher than that in CF. The slopes of soil organic matter and total nitrogen over six years were the highest in 60C and 60D. The soil total nitrogen content in 60A, 60B, 60C and 60D was higher than that in CF, but the differences were not significant (P>0.05). Therefore, a relatively high Chinese milk vetch incorporation rate (≥30 Mg ha^–1) was more effective in improving the productivity and sustainability of paddy soil. The decreased coefficients of early-rice yield and the Chinese milk vetch incorporation rate in 2014 implied that the benefits of soil fertility and rice yield created by Chinese milk vetch input may decline after five years under a continuously high rate of Chinese milk vetch incorporation.

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Strawberry vein banding virus P6 protein intracellular transport and an important domain identification

PAN Yuan, ZHOU Xiu-hong, LI Shuai, FENG Ming-feng, SHI Man-ling, ZUO Deng-pan, JIANG Xi-zi, CHEN Jing, HU Ya-hui, ZHANG Xiang-xiang, JIANG Tong

2018, 17 (09): 2031-2041. DOI: 10.1016/S2095-3119(18)61978-1

Abstract （376） PDF （13957KB）（271）

Strawberry vein banding virus (SVBV)-infected strawberry cells contain cytoplasmic inclusions with isometric particles. To identify the components of the inclusions, green fluorescent protein (GFP) was fused to the carboxy-terminus (C-terminus) of SVBV open reading frames, these constructs were separately transformed into Agrobacterium tumefaciens and infiltrated into Nicotiana benthamiana leaves. Results showed that the SVBV P6 protein assembled into prominent and amorphous inclusion bodies (IBs). To investigate P6 subcellular localization, P6-GFP was ectopically expressed in N. benthamiana leaves by agroinfiltration and then stained with 4´,6-diamidino-2-phenylindole (DAPI). We found the P6 protein accumulated in the nuclei and also formed cytoplasmic IBs with different sizes. To further determine the location of P6 IBs in the cytoplasm, and explore whether the P6 IBs move freely or depend on cytoskeleton and endoplasmic reticulum (ER), the microfilament marker protein (GFP-ABD2-GFP), microtubules marker protein (mCherry-MAP65-1) and ER marker protein (mCherry-HDEL) were separately coexpressed with P6-GFP and into N. benthamiana leaves by agroinfiltration, exhibiting that P6 IBs aligned with cytoskeleton and endoplasmic reticulum. Meanwhile, coinfiltration of P1 and P6 indicated the P6 colocalized with the P1 protein at periphery of cells. The P6 protein contains one C-terminal nuclear localization signal (NLS) region, a P6 protein mutant with a deleted NLS did not localize in the nucleus, did not form IBs, and was unable to facilitate exogenous GFP expression. These results demonstrate that the deleted NLS region is an important P6 domain required for biological functions. In summary, the mobile P6 IBs are associated with ER, microfilaments and microtubules and move along microfilaments to the SVBV P1 protein in the PD.

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Using a process-oriented methodology to precisely evaluate temperature suitability for potato growth in China using GIS

HE Ying-bin, ZHOU Yang-fan, CAI Wei-min, WANG Zhuo-zhuo, DUAN Ding-ding, LUO Shan-jun, CHEN Jing-zhu

2017, 16 (07): 1520-1529. DOI: 10.1016/S2095-3119(16)61627-1

Abstract （813） PDF in ScienceDirect

A process-oriented methodology to conduct precise evaluation temporally and spatially on temperature suitability for potato growth was applied in China. Arable lands in China were gridded with 1 km×1 km geographic units, and potential potato phenology in each unit was automatically identified in terms of the potato planting initial temperature and effective accumulated temperature. A temperature thermal response coefficient model was used to compute a temperature suitability value for each day of potato phenology in each geographic unit. In addition, five temperature suitability ranking methods were applied to define suitable areas: (1) upper fourth quantile, (2) median, (3) expected value+1/4 standard deviation, (4) expected value+1/2 standard deviation, (5) expected value+1 standard deviation. A validation indicator was innovated to test the effectiveness of the five ranking methods. The results showed that from a strict degree point of view, the five methods sequence was as follows: 1=3>4>2>5, with a and c determined as the two best ranking methods. For methods 1 and 3, the suitable potato growing area was 1 of 57.76×10⁴ km². In addition, the suitable areas were spatially coincident with the main potato producing counties. The study output technically supports the proposal from China’s government that there is a large potential area to grow winter-ploughed potato in South China because the potential suitable area for growing potato is approximately 2×10⁷ ha. In southeast Heilongjiang and east Jilin, where it is hilly and mountainous, there are still some potentially suitable areas for potato growing accounting for nearly 2.32×10⁶ ha. The authors suggest to optimize the agricultural regionalization and layout in China and to adjust the cropping pattern structure.

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Influence of potato flour on dough rheological properties and quality of steamed bread

LIU Xing-li, MU Tai-hua, SUN Hong-nan, ZHANG Miao, CHEN Jing-wang

2016, 15 (11): 2666-2676. DOI: 10.1016/S2095-3119(16)61388-6

Abstract （2236） PDF in ScienceDirect

It is a novel idea to make steamed bread by adding potato flour into wheat flour considering the production and nutritional factors of potato. In this study, the influence of potato flour (0–35%) on dough rheology and quality of steamed bread were investigated. Potato flour addition significantly influenced the dough rheological properties and steamed bread quality, such as increased water absorption, the maximum gaseous release height, total volume of CO₂ and hardness, while decreased dough stability and specific volume of steamed bread. Moreover, correlation analysis suggested that dough height at the maximum development time, dough stability, water absorption and the phase tangent can be used for predicting the technological quality of steamed bread. Potato-wheat steamed bread had higher dietary fibre, ash content and antioxidant activity than those of wheat steamed bread. The estimated glycemic index decreased from 73.63 (0%) to 60.01 (35%). Considering the sensory evaluation, the steamed bread with 20% potato flour is acceptable. In conclusion, adding appropriate quantity of potato flour to wheat flour for steamed bread production will not only maintain the technological quality, but also can improve the nutritional value of the steamed bread.

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Characterization of glyphosate-resistant goosegrass (Eleusine indica) populations in China

CHEN Jing-chao, HUANG Hong-juan, WEI Shou-hui, ZHANG Chao-xian, HUANG Zhao-feng

2015, 14 (5): 919-925. DOI: 10.1016/S2095-3119(14)60910-2

Abstract （2561） PDF in ScienceDirect

Goosegrass is a worst grass weed in orchards and turf. The increased use of glyphosate for goosegrass control has led to the occurrence of many resistant populations. Although glyphosate has been used to control weeds for the past 30 years in China, few reports are available on glyphosate-resistant (GR) googegrass. In this study, we determined the GR level of 14 goosegrass populations from Chengdu and Guangzhou, China. Glyphosate only controlled 3.1 and 25.0% of the populations SL5 and SL1, respectively, at the dose of 1 680 g acid equivalent (ae) ha–1 at 14 days after treatment (DAT). In contrast, the susceptible population (XD1) was completely (100%) controlled. The resistant index (RI) of SL5 and SL1 were 5.1 and 4.5, and the RI for SL2, SL3 and ZC1 were 4.2, 3.2 and 2.6, respectively. The RI for other populations was range from 1.8 to 2.5. Under the dose of glyphosate at 1 640 g ae ha–1 at 10 DAT, shikimate accumulation in susceptible population XD1 was 17.6 and 16.4 times higher than SL5 and SL1, respectively. And the chlorophyll content in the plant leaf of populations SL1, SL2 and SL5 were decreased slightly ranging from 22.6 to 28.0. These results confirmed that the SL1, SL2, SL3, ZC1 and SL5 populations had evolved moderate resistance to glyphosate. This is the first report for the GR goosegrass populations confirmed in Chengdu, China.

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QTL Mapping for Stalk Related Traits in Maize (Zea mays L.) Under Different Densities

ZHU Li-ying, CHEN Jing-tang, Li Ding, ZHANG Jian-hua, HUANG Ya-qun, ZHAO Yong-feng, SONG Zhan-quan , LIU Zhi-zeng

2013, 12 (2): 218-228. DOI: 10.1016/S2095-3119(13)60221-X

Abstract （1522） PDF in ScienceDirect

Stalk related traits, comprising plant height (PH), ear height (EH), internode number (IN), average internode length (AIL), stalk diameter (SD), and ear height coefficient (EHC), are significantly correlated with yield, density tolerance, and lodging resistance in maize. To investigate the genetic basis for stalk related traits, a doubled haploid (DH) population derived from a cross between NX531 and NX110 were evauluated under two densities over 2 yr. The additive quantitative trait loci (QTLs), epistatic QTLs were detected using inclusive composite interval mapping and QTL-by-environment interaction were detected using mixed linear model. Differences between the two densities were significant for the six traits in the DH population. A linkage map that covered 1 721.19 cM with an average interval of 10.50 cM was constructed with 164 simple sequence repeat (SSR). Two, two, seven, six, two, and eight additive QTLs for PH, IN, AIL, EH, SD, and EHC, respectively. The extend of their contribution to penotypic variation ranged from 10.10 to 31.93%. Seven QTLs were indentified simultaneously under both densities. One pair, two pairs and one pair of epistatic effects were detected for AIL, SD and EHC, respectively. No epistatic effects were detected for PH, EH, and IN. Nineteen QTLs with environment interactions were detected and their contribution to phenotypic variation ranged from 0.43 to 1.89%. Some QTLs were stably detected under different environments or genetic backgrounds comparing with previous studies. These QTLs could be useful for genetic improvement of stalk related traits in maize breeding.

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Diversity, Structure, and Marker-Trait Association Analysis of the Maize Recombinant Inbred Line Population

CHEN Jing-tang; HU Li-zong; ZHU Li-ying; GUO Jin-jie; ZHAO Yong-feng and HUANG Ya-qun

2011, 10 (7): 975-986. DOI: 10.1016/S1671-2927(11)60084-9

Abstract （1688） PDF in ScienceDirect

Association mapping has emerged as a new tool to elucidate complex quantitative trait loci in maize, but there are fewreports about systematic association analysis for the specific SSR markers with agronomic traits of interest in China.We investigated the morphological and genetic diversity and population structure for 76 maize recombinant inbredlines, and then association analysis were further performed between 48 simple sequence repeat loci and 17 morphologicaltraits, consisting of nine ear-related traits and eight other traits. The 48 SSR markers were screened out and furtherclassified into two groups including a group of loci in regions harboring reported quantitative trait loci that affect earshape and a group of markers distributing on the whole genome randomly. The result indicated that the population ofrecombinant inbred lines was structured, showing five subpopulations. Our association results revealed that therewere 82, 59, and 40 significant associations detected by K-test, logistic regression, and both analysis, respectively.When the 17 traits were considered separately, the significant associations between Q-SSRs and E-traits were raised to27.8%, whereas the other groups of combinations ranged between 2.3 and 6.3%. As the proportion of significantassociations is higher among the Q-SSR subset of markers and the subset of traits related to ear shape than those forall of the other combinations, we conclude that this approach is valid for establishing true positive marker-traitrelationships. Our results also demonstrated that association mapping could complement and enhance previous QTLinformation for marker-assisted selection.

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Identification of QTLs for plant height and branching related traits in cultivated peanut

ZHANG Sheng-zhong, HU Xiao-hui, WANG Fei-fei, MIAO Hua-rong, Ye Chu, YANG Wei-qiang, ZHONG Wen, CHEN Jing

DOI: 10.1016/j.jia.2023.12.009 Online: 15 December 2023

Abstract （74） PDF in ScienceDirect

Plant height (PH), primary lateral branch length (PBL) and branch number (BN) are architectural components impacting peanut pod yield, biomass production and adaptivity to mechanical harvesting. In this study, a recombinant inbred population consisting of 181 individual lines was used to determine genetic controls of PH, PBL and BN across three environments. Phenotypic data collected from the population demonstrated continuous distributions and transgressive segregation patterns. Broad-sense heritability of PH, PBL and BN was found to be 0.87, 0.88 and 0.92, respectively. Unconditional individual environmental analysis revealed 35 additive QTLs with phenotypic variation explained (PVE) ranging from 4.57 to 21.68%. A two-round meta-analysis resulted in 24 consensus and 17 unique QTLs. Five unique QTLs exhibited pleiotropic effects and their genetic bases (pleiotropy or tight linkage) were evaluated. Joint analysis was performed to estimate the QTL by environment interaction (QEI) effects on PH, PBL and BN, which collectively explained phenotypic variations of 10.80, 11.02, and 7.89%, respectively. We identified 3 major and stable QTL regions (uq9-3, uq10-2 and uq16-1) on chromosomes 9, 10 and 16, spanning 1.43-1.53 Mb genomic regions. Candidate genes involved in phytohormones biosynthesis, signaling and cell wall development were proposed to regulate these morphological traits. These results provide valuable information for further genetic studies and development of molecular markers applicable for peanut architecture improvement.

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