|
|
|
|
|
| Accumulation of Carbohydrate and Regulation of 14-3-3 Protein on Sucrose Phosphate Synthase (SPS) Activity in Two Tomato Species |
| WANG Li, CUI Na, ZHAO Xiao-cui, FAN Hai-yan , LI Tian-lai |
1.Biological Science and Technology College, Shenyang Agricultural University, Shenyang 110866, P.R.China
2.Key Laboratory of Protected Horticulture, Ministry of Education/College of Horticulture, Shenyang Agricultural University, Shenyang 110866,
P.R.China |
|
|
|
摘要 To explore the differences of carbohydrate metabolism in two tomato species and discuss the possible regulation of 14-3-3 proteins on the sucrose phosphate synthase (SPS) activity, we determined the contents of soluble sugar and starch through high performance liquid chromatography (HPLC). The activities of sugar-metabolizing enzymes were assayed in desalted extract, and the relative expression levels of related genes in sugar metabolism were determined though real-time RT-PCR. The results indicated that glucose and fructose were mainly accumulated during the maturation of the fruit because of the high acid invertase (AI) and neutral invertase (NI) in Micro-Tom (Solanum lycopersicum) fruit, while in Solanum chmielewskii fruit, SPS which went along with the change of sucrose content led to the rapid sucrose increase during the fruit ripening. TFT1 and TFT10, belonging to 14-3-3 protein in tomato, were likely to down-regulated SPS activity during young and intumescence period.
Abstract To explore the differences of carbohydrate metabolism in two tomato species and discuss the possible regulation of 14-3-3 proteins on the sucrose phosphate synthase (SPS) activity, we determined the contents of soluble sugar and starch through high performance liquid chromatography (HPLC). The activities of sugar-metabolizing enzymes were assayed in desalted extract, and the relative expression levels of related genes in sugar metabolism were determined though real-time RT-PCR. The results indicated that glucose and fructose were mainly accumulated during the maturation of the fruit because of the high acid invertase (AI) and neutral invertase (NI) in Micro-Tom (Solanum lycopersicum) fruit, while in Solanum chmielewskii fruit, SPS which went along with the change of sucrose content led to the rapid sucrose increase during the fruit ripening. TFT1 and TFT10, belonging to 14-3-3 protein in tomato, were likely to down-regulated SPS activity during young and intumescence period.
|
|
Received: 10 December 2012
Accepted:
|
| Fund: This study was supported by the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2011BAD12B03). |
|
Corresponding Authors:
CUI Na, Tel: +86-24-88487163, E-mail: syaua@163.com
E-mail: syaua@163.com
|
| About author: WANG Li, E-mail: sdwl881209@yahoo.com.cn |
Cite this article:
WANG Li, CUI Na, ZHAO Xiao-cui, FAN Hai-yan , LI Tian-lai.
2014.
Accumulation of Carbohydrate and Regulation of 14-3-3 Protein on Sucrose Phosphate Synthase (SPS) Activity in Two Tomato Species. Journal of Integrative Agriculture, 13(2): 358-364.
|
| Aitken A. 2006. 14-3-3 proteins: A historic overview Semin Cancer Biology, 16, 162-172.Alexander R D, Morris P C. 2000. A proteomic analysis of 14-3-3 binding proteins from developing barley grains Proteomics, 6, 1886-1896. Bornke F. 2005. The variable C-terminus of 14-3-3 proteins mediates isoform-specific interaction with sucrose- phosphate synthase in the yeast two-hybrid system Plant Physiology, 162, 161-168.Chai Y M, Jia H F, Li C L, Qin L, Shen Y Y. 2011. Transcriptional analysis of sugar metabolism-related genes during strawberry fruit development. Acta Horticulturae Sinica, 38, 637-643. (in Chinese) Chen L L, WangT, Huang X Y, Lu C Y, Liu D F, Zheng X Y, Teng Y W. 2011. Study on enzyme activities involved in sugar accumulation and sucrose metabolization in Cuiguan Pear under plastic tunnel culture. Journal of Fruit Science, 28, 400-405 Chen S, Hajirezaei M, Bnrnke F. 2005. Differential expression of sucrose-phosphate synthase isoenzymes in tobacco reflects their functional specialization during dark-governed starch mobilization in source leaves. Plant Physiology, 139, 1163. Cui N, Li T L, Li Y. 2007. The main action of 14-3-3 proteins in plants Biotechnology, 17, 86. Daphne M, Arthur A S. 1991. Sucrose phosphate synthase, sucrose synthase, and invertase activities in developing fruit of Lycopersicon esculentum Mill. and the sucrose accumulating Lycopersicon hirsutum Humb. and Bonpl. Plant Physiology, 95, 623-627 Fararr J, Pollock C, Gallagher J. 2000. Sucrose and the integration of metabolism in vascular plants. Plant Science, 154, 1-11 Islam M S, Toshiyuki M, Yoshida Y. 1996. Carbohydrate content and the activities of sucrose synthase, sucrose phosphate synthase and acid invertase in different tomato cultivars during fruit development. Scientia Horticulturae, 65, 125-136 Kühn C, Grof C P. 2010. Sucrose transporters of higher plants. Plant Biology, 13, 288-298 Liu Y Q, Shen H L, Shi Z Q. 2006. The sugar metabolism in tomato developing fruits. Acta Horticulturae Sinica, 21, 51-56 (in Chinese) Niu S. 1992. Quality Analysis of Crops. 1st ed. China Agriculture Press, Beijing. (in Chinese) Oh C S, Pedley K F, Martin G B. 2010. Tomato 14-3-3 protein 7 positively regulates immunity-associated p rogrammed cell death by enhancing protein abundance and signaling ability of MAPKKKa The Plant Cell, 22, 260-272. Purwestri Y A, Ogaki Y, Tamaki S, Tsuji H, Shimamoto K. 2009. The 14-3-3 protein GF14c acts as a negative regulator of flowering in rice by interacting with the florigen Hd3a Plant Cell Physiology, 50, 429-438. Qi H Y, Li T L, Zhang J, Liu H T. 2006. Relationship between carbohydrate change and related enzymes activities during tomato fruit development. Acta Horticulturae Sinica, 33, 294-299. (in Chinese) Stitt M, Wilke I, Feil R, Heldt H W. 1998. Coarse control of sucrose phosphate synthase in leaves: Alterations of the kinetic properties in response to the rate of photosynthesis and the accumulation of sucrose. Planta, 174, 217-230 Toroser D, Athwal G S, Huber S C. 1988. Site-specific regulatory interaction between spinach leaf sucrose phosphate synthase and 14-3-3 proteins FEBS Letters, 435, 110-114. Wang Y Z, Zhang D P. 2000. The regulation of carbohydrate in “Hong-Xing” mature fruit from ethylene. Horticulturae Sinica, 27, 391-395. (in Chinese) Wind J, Smeekens S, Hanson J. 2010. Sucrose: Metabolite and signaling molecule. Phytochemistry, 71, 1610-1614 Xu W F, Shi W M. 2009. Expression profiling of the 14-3- 3 gene family in response to salt stress and potassium and iron deficiencies in young tomato (Solanum lycopersicum) roots: Analysis by real-time RT-PCR Annals of Botany, 98, 965-974. Yan M L. 2009. Research progress of the influence of sucrose-metabolizing enzymes on the sugar accumulation in fruit. Journal of Anhui Agricultral University, 37, 14021-14023, 14027. (in Chinese) Yu N W, Li J C, Wang J Z, Cai Z M, Sha S F, Li H J. 2011. Roles of related enzymes in accumulation of sugars in Nanguoli Pear fruit. Southwest China Journal of Agricultural Sciences, 24, 091. (in Chinese) Yu X J. 1985. Plant Physiology Experiments Manual. Shanghai Science and Technology Press, Shanghai. pp. 148-149. (in Chinese) Yu Z H, Cui N. 2010. The prognosis of interaction between 14-3-3 protein and SPS in tomato. Acta Horticulturae Sinica, 37, 2150. (in Chinese) Zhang F M, Li Z L. 2002. Related sucrose-metabolizing enzymes in higher plants. Plant Physiology Communications, 38, 289-295. Zuk M, Weber R, Szopa J. 2005. 14-3-3 protein down- regulates key enzyme activities of nitrate and carbohydrate metabolism in potato plants. Journal of Agricultural and Food Chemistry, 53, 3454-3460. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
