|
|
|
|
|
| Identification of a Group of Novel γ-Gliadin Genes |
| QI Peng-fei, WEI Yu-ming, Ouellet Thérèse, CHEN Qing, WANG Zhao, WEI Zhen-zhen , ZHENG You-liang |
1.Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
2.Agriculture & Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, Ottawa K1A 0C6, Canada |
|
|
|
摘要 γ-Gliadins are an important component of wheat seed storage proteins. Four novel γ-gliadin genes (Gli-ng1 to Gli-ng4) were cloned from wheat (Triticum aestivum) and Aegilops species. The novel γ-gliadins were much smaller in molecular size when compared to the typical γ-gliadins, which was caused by deletion of the non-repetitive domain, glutamine-rich region, 3´ part of the repetitive domain, and 5´ part of the C-terminal, possibly due to illegitimate recombination between the repetitive domain and the C-terminal. As a result, Gli-ng1 and Gli-ng4 only contained two and three cysteine residues, respectively. Gli-ng1, as the representative of novel γ-gliadin genes, has been sub-cloned into an Escherichia coli expression system. SDS- PAGE indicated that the both cysteine residues of Gli-ng1 could participate in the formation of intermolecular disulphide bonds in vitro. Successful cloning of Gli-ng1 from seed cDNA of T. aestivum cv. Chinese Spring suggested that these novel γ-gliadin genes were normally transcribed during the development of seeds. Phylogenic analysis indicated that the four novel γ-gliadin genes had a closer relationship with those from the B (S) genome of wheat.
Abstract γ-Gliadins are an important component of wheat seed storage proteins. Four novel γ-gliadin genes (Gli-ng1 to Gli-ng4) were cloned from wheat (Triticum aestivum) and Aegilops species. The novel γ-gliadins were much smaller in molecular size when compared to the typical γ-gliadins, which was caused by deletion of the non-repetitive domain, glutamine-rich region, 3´ part of the repetitive domain, and 5´ part of the C-terminal, possibly due to illegitimate recombination between the repetitive domain and the C-terminal. As a result, Gli-ng1 and Gli-ng4 only contained two and three cysteine residues, respectively. Gli-ng1, as the representative of novel γ-gliadin genes, has been sub-cloned into an Escherichia coli expression system. SDS- PAGE indicated that the both cysteine residues of Gli-ng1 could participate in the formation of intermolecular disulphide bonds in vitro. Successful cloning of Gli-ng1 from seed cDNA of T. aestivum cv. Chinese Spring suggested that these novel γ-gliadin genes were normally transcribed during the development of seeds. Phylogenic analysis indicated that the four novel γ-gliadin genes had a closer relationship with those from the B (S) genome of wheat.
|
|
Received: 07 January 2013
Accepted:
|
| Fund: This research was finically supported by the National Natural Science Foundation of China (31230053). |
|
Corresponding Authors:
ZHENG You-liang, Tel: +86-835-2882007, Fax: +86-835-2882153, E-mail: ylzheng@sicau.edu.cn
E-mail: ylzheng@sicau.edu.cn
|
| About author: QI Peng-fei, E-mail: pengfeiqi@hotmail.com |
Cite this article:
QI Peng-fei, WEI Yu-ming, Ouellet Thérèse, CHEN Qing, WANG Zhao, WEI Zhen-zhen , ZHENG You-liang.
2014.
Identification of a Group of Novel γ-Gliadin Genes. Journal of Integrative Agriculture, 13(2): 290-298.
|
| Akhunov E D, Goodyear A W, Geng S, Qi L L, Echalier B, Gill B S, Miftahudin J, Gustafson J P, Lazo G, Chao S, et al. 2003. The organization and rate of evolution of wheat genomes are correlated with recombination rates along chromosome arms. Genome Research, 13, 753- 763. Anderson O D, Hisa C C, Torres V. 2001. The wheat γ-gliadin genes: characterization of ten new sequences and further understanding of γ-gliadin gene family structure. Theoretical and Applied Genetics, 103, 323- 330. Bulleid N J, Freedman R B. 1988. The transcription and translation in vitro of individual cereal storage-protein genes from wheat (Triticum aestivum cv. Chinese Spring). Biochemical Journal, 254, 805-810 Clarke B C, Phongkham T, Gianibelli M C, Beasley H, Bekes F. 2003. The characterization and mapping of a family of LMW-gliadin genes: Effects on dough properties and bread volume. Theoretical and Applied Genetics, 106, 629-635 Doyle J J, Doyle J L. 1987. A rapid DNA isolation procedure from small quantities of fresh leaf tissues. Phytochemical Bulletin, 19, 11-15 Fido R J, Bekes F, Gras P W, Tatham A S. 1997. Effects of α-, β-, γ- and ω-gliadins on the dough mixing properties of wheat flour. Journal of Cereal Science, 26, 271-277 Galili G. 1989. Heterologous expression of a wheat high molecular weight glutenin gene in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America, 86, 7756-7760 Ji L, Barrett T, Ayanbule O, Troup D B, Rudnev D, Muertter R N, Tomashevsky M, Soboleva A, Slotta D J. 2010. NCBI Peptidome: a new repository for mass spectrometry proteomics data. Nucleic Acids Research, 38, D731-D735. Kumar S, Tamura K, Nei M. 2004. MEGA 3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in Bioinformatics, 5, 150-163 L e w E J L, Kuzmicky D D, Kasarda D D. 1992.Characterization of low-molecular-weight gluteninsubunits by reversed-phase high-performance liquidchromatography, sodium dodecyl sulfate-polyacrylamidegel electrophoresis, and N-terminal amino-acidsequencing. Cereal Chemistry, 69, 508-515Li R X, Chen H B, Tu K, Zhao S L, Zhou H, Li S J, Dai J,Li Q R, Nie S, Li Y X, et al. 2008. Localized-statisticalquantification of human serum proteome associated withtype 2 diabetes. PLoS One, 3, e3224.McFadden E S, Sears E R. 1946. The origin of Triticumspelta and its free-threshing hexaploid relatives. Journalof Heredity, 37, 81-89, 107-116Masci S, Lew E J L, Lafiandra D, Porceddu E, Kasarda D D. 1995. Characterization of low molecular weight glutenin subunits in durum wheat by reversed-phase high-performance liquid chromatography and N-terminalsequencing. Cereal Chemistry, 72, 100-104Müller S, Wieser H, Popineau Y. 1998. Disulphide bonds ofγ46-gliadin. Journal of Cereal Science, 27, 23-25Payne P I. 1987. Genetics of wheat storage proteins andthe effect of allelic variation on bread-making quality.Annual Review of Plant Physiology, 38, 141-153Payne P I, Holt L M, Jackson E A, Law C N. 1984.Wheat storage proteins: their genetics and potential for manipulation by plant breeding. PhilosophicalTransactions of the Royal Society (B: Biological Sciences), 304, 359-371Pollitt S, Zalkin H. 1983. Role of primary structure anddisulfide bond formation in β-lactamase secretion.Journal of Bacteriology, 153, 27-32Qi P F, Wei Y M, Chen Q, Ouellet T, Ai J, Chen G Y, LiW, Zheng Y L. 2011. Identification of novel α-gliadingenes. Genome, 54, 244-252Qi P F, Wei Y M, Ouellet T, Chen Q, Tan X, Zheng Y L.2009. The γ-gliadin multigene family in common wheat(Triticum aestivum) and its closely related species. BMCGenomics, 10, 168.Qi P F, Wei Y M, Yue Y W, Yan Z H, Zheng Y L. 2006.Biochemical and molecular characterization of gliadins.Molecular Biology, 40, 713-723Sabelli P, Shewry P R. 1991. Characterization andorganization of gene families at the Gli-1 loci of breadand durum wheats by restriction fragment analysis Theoretical and Applied Genetics, 83, 209-216Salcedo G, Prada J, Aragoncillo C. 1979. Low-molecular-weight gliadin-like proteins from wheat endosperm.Phytochemistry, 18, 725-727Shewry P R, Tatham A S. 1990. The prolamin storage proteins of cereal seeds: Structure and evolution.Biochemical Journal, 267, 1-12Wicker T, Yahiaoui N, Guyot R, Schlagenhauf E, Liu Z D,Dubcovsky J, Keller B. 2003. Rapid genome divergenceat orthologous low molecular weight glutenin loci of A and Am genomes of wheat. The Plant Cell, 15, 1186-1197Zhang Y, Li X, Wang A, An X, Zhang Q, Pei Y, Gao L,Ma W, Appels R, Yan Y 2008. Novel x-type high-molecular-weight glutenin genes from Aegilops tauschiiand their implications on the wheat origin and evolutionmechanism of Glu-D1-1 proteins Genetics, 178, 23-33. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
