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| Functional Analysis of the Phosphoenolpyruvate Carboxylase on the Lipid Accumulation of Peanut (Arachis hypogaea L.) Seeds |
| PAN Li-juan, YANG Qing-li, CHI Xiao-yuan, CHEN Ming-na, YANG Zhen, CHEN Na, WANG Tong, WANG Mian, HE Ya-nan, YU Shan-lin |
| Shandong Peanut Research Institute, Qingdao 266100, P.R.China |
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摘要 Phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) catalyses phosphoenolpyruvate (PEP) to yield oxaloacetate, which is involved in protein biosynthesis. Pyruvate kinase (PK; EC 2.7.1.40) catalyzes PEP to yield pyruvate, which is involved in fatty acid synthesis. In this study, five PEPC genes (AhPEPC1, AhPEPC2, AhPEPC3, AhPEPC4, and AhPEPC5) from peanut have been cloned. Using a quantitative real-time RT-PCR approach, the expression pattern of each gene was monitored during the seed development of four peanut varieties (E11, Hebeigaoyou, Naihan 1, and Huayu 26). It was found that these five genes shared similar expression behaviors over the developmental stages of E11 with high expression levels at 30 and 40 d after pegging (DAP); whereas these five genes showed irregular expression patterns during the seed development of Hebeigaoyou. In Naihan 1 and Huayu 26, the expression levels of the five genes remained relatively high in the first stage. The PEPC activity was monitored during the seed development of four peanut varieties and seed oil content was also characterized during whole period of seed development. The PEPC activity followed the oil accumulation pattern during the early stages of development but they showed a significantly negative correlation thereafter. These results suggested that PEPC may play an important role in lipid accumulation during the seed development of four peanut varieties tested.
Abstract Phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) catalyses phosphoenolpyruvate (PEP) to yield oxaloacetate, which is involved in protein biosynthesis. Pyruvate kinase (PK; EC 2.7.1.40) catalyzes PEP to yield pyruvate, which is involved in fatty acid synthesis. In this study, five PEPC genes (AhPEPC1, AhPEPC2, AhPEPC3, AhPEPC4, and AhPEPC5) from peanut have been cloned. Using a quantitative real-time RT-PCR approach, the expression pattern of each gene was monitored during the seed development of four peanut varieties (E11, Hebeigaoyou, Naihan 1, and Huayu 26). It was found that these five genes shared similar expression behaviors over the developmental stages of E11 with high expression levels at 30 and 40 d after pegging (DAP); whereas these five genes showed irregular expression patterns during the seed development of Hebeigaoyou. In Naihan 1 and Huayu 26, the expression levels of the five genes remained relatively high in the first stage. The PEPC activity was monitored during the seed development of four peanut varieties and seed oil content was also characterized during whole period of seed development. The PEPC activity followed the oil accumulation pattern during the early stages of development but they showed a significantly negative correlation thereafter. These results suggested that PEPC may play an important role in lipid accumulation during the seed development of four peanut varieties tested.
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Received: 28 December 2011
Accepted:
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| Fund: This study was supported by the China Agriculture Research System (CARS-14), the National Natural Science Foundation of China (31000728, 31100205), the Natural Science Funda t ion of Shangdong Province, China (ZR2009DQ004, ZR2011CQ036), the Promotive Research Fund for Young and Middle-Aged Scientisits of Shandong Province, China (BS2010NY023), and the Qingdao Municipal Science and Technology Plan Project, China (11-2-4-9- (3)-jch, 11-2-3-26-nsh). |
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Corresponding Authors:
Correspondence YU Shan-lin, Tel: +86-532-87626672, E-mail: yshanlin1956@163.com
E-mail: yshanlin1956@163.com
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Cite this article:
PAN Li-juan, YANG Qing-li, CHI Xiao-yuan, CHEN Ming-na, YANG Zhen, CHEN Na, WANG Tong, WANG Mian, HE Ya-nan, YU Shan-lin.
2013.
Functional Analysis of the Phosphoenolpyruvate Carboxylase on the Lipid Accumulation of Peanut (Arachis hypogaea L.) Seeds. Journal of Integrative Agriculture, 12(1): 36-44.
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| [1]Andre C, Froehlich J E, Moll M R, Benning C. 2007. Aheteromeric plastidic pyruvate kinase complex involvedin seed oil biosynthesis in Arabidopsis. The Plant Cell,19, 2006-2022[2]Andreo C S, Gonzalez D H, Iglesias AA 1987. Higher plantphosphoenolpyruvate carboxylase: structure andregulation. FEBS Letters, 213, 1-8[3]Araus J L, Bort J, Brow R H, Bassett C L, Cortadellas N1993. Immunocytochemi calloc alization ofphosphoenolpyruvate carboxylase and photosynthesisgas-exchange characteristics in ears of Triticum durumDesf. Planta, 191, 507-514[4]Atkins C A, Pate J S, Sharkey P J. 1975. Asparaginemetabolism key to the nitrogen nutrition of developinglegume seeds. Plant Physiology, 56, 807-812[5]Botha F C, Dennis D T. 1986. Phosphoglyceromutaseactivity and concentration in the endosperm ofdeveloping and germinating Ricinus communis seeds.Canadian Journal of Botany-Revue Canadienne deBotanique, 65, 1908-1912[6]Chi X Y, Yang Q L, Pan L J, Chen M N, He Y N, Yang Z, YuS L. 2011. Isolation and characterization of fatty aciddesaturase genes from peanut (Arachis hypogaea L.).Plant Cell Reports, 30, 1393-1404[7]Chollet R, Vidal J, O’LearyMH. 1996. Phosphoenolpyruvatecarboxylase: a ubiquitous, highly regulated enzyme inplants. Annual Review of Plant Physiololy and PlantMoecularl Biology, 47, 273-298[8]FlinnAM. 1985. Carbon dioxide fixation in developing seeds.In: Hebblethwaite P D, Heath M C, Dawkins T C K, eds.,The Pea Crop. Butterworths, London. pp. 349-358[9]Gehrig I H, Heute V, Kluge M. 1998. Toward a betterknowledge of the mol e cul a r evolut ion o fphosphoenolpyruvate carboxylase by comparison ofpartial cDNA sequences. Journal of MolecularEvolution, 46, 107-114[10]Gennidakis S, Rao S, Greenham K, Uhrig R G, O’Leary B,Snedden W S, Lu C, Plaxton W C. 2007. Bacterial- andplant -type phosphoenolpyruvate carboxylasepolypeptides interact in the hetero-oligomeric class-2PEPC complex of developing castor oil seeds[11]The PlantJournal, 52, 839-849[12]González M C, Osuna L, Echevarria C, Vidal J, Cejudo F J.1998. Expression and localization of PEP carboxylase indeveloping and germinating wheat grains. PlantPhysiology, 116, 1249-1258[13]Harwood H. 1984. Oleochemicals as a fuel. Mechanical andeconomic feasibility. Journal of the American OilChemists Society, 61, 315-324[14]Hedley C L, Harvey D M, Keely R J. 1975. Role of PEPcarboxylase during seed development in Pisum sativum.Nature, 258, 352-354[15]Knauft D A, Ozias-Akins P. 1995. Recent methodologiesfor germplasm enhancement and breeding. In: Pattee HE, Stalker H T, eds., Advances in Peanut Science.American Peanut Research and Education Society,Stillwater, OK. pp. 54-94[16]Lepiniec L, Vidal J, Chollet R, Gadal P, Crétin C. 1994.Phosphoenolpyruvate carboxylase: st ructure,regulation and evolution. Plant Science, 99, 111-124[17]Mamedov T G, Moellering E R, Chollet R. 2005.Identification and expression analysis of two inorganicC- and N-responsive genes encoding novel and distinctmolecular forms of eukaryotic phosphoenolpyruvatecarboxylase in the green microalga Chlamydomonasreinhardtii. The Plant Journal, 42, 832-843[18]Masumoto C, Miyazawa S I, Ohkawa H, Fukuda T,Taniguchi Y, Murayama S, Kusano M, Saito K,Fukayama H, Miyao M. 2010. Phosphoenolpyruvatecarboxylase intrinsically located in the chloroplast ofrice plays a crucial role in ammonium assimilation.Proceedings of the National Academy of Sciences ofthe United States of Amarica, 107, 5226-5231[19]Murray D R, Cordova-Edwards M. 1984. Amino acid andamide metabolism in the hulls and seeds of developingfruits of garden pea, Pisum sativum. I. Glutamine. NewPhytologist, 97, 243-252[20]PlaxtonWC, Podestá F E. 2006. The functional organizationand control of plant respiration. Critical Reviews inPlant Sciences, 25, 159-198[21]Ruuska S A, Girke T, Benning C, Ohlrogge J B. 2002.Contrapuntal networks of gene expression duringArabidopsis seed filling. The Plant Cell, 14, 1191-1206[22]Sabine G, Ute H, Christian H, Ulrich W, Hans W. 1999.Phosphoenolpyruvate carboxylase in developing seedsof Vicia faba L.: gene expression and metabolicregulation. Planta, 208, 66-72[23]Sánchez R, Cejudo F J. 2003. Identification and expressionanalysis of a gene encoding a bacterial-typephosphoenolpyruvate carboxylase from Arabidopsisand rice. Plant Physiology, 132, 949-957[24]Sangwan R S, SinghN, PlaxtonWC. 1992. Phosphoenolpyruvatecarboxylase activity and concentration in the endosperm ofdeveloping and germinating castor oil seeds. PlantPhysiology, 99, 445-449[25]Sebei K, Ouerghi Z, Kallel H, Boukhchina S. 2006. Evolutionof phosphoenolpyruvate carboxylase activity and lipidcontent during seed maturation of two spring rapeseedcultivars. Comptes Rendus Biologies, 329, 719-725[26]Singal H R, Sheron J S, Singh R. 1987. Photosyntheticcarbon fixation characteristic of fruiting structures ofBrassica napus L. Plant Physiology, 83, 1043-1047[27]Smith A J, Rinne R W, Seif R D. 1989. PEP carboxylase andpyruvate kinase involvement in protein and oilbiosynthesis during soybean seed development. CropScience, 29, 349-353[28]Sugimoto T, Tanaka K, Monma M, Kawamura Y, Saio K.1989. Phosphoenolpyruvate carboxylase level insoybean seed highly correlates to its contents of proteinand lipid. Agricultural Biology Chemistry, 53, 885-887[29]Toh H, Kawamura T, Izui K. 1994. Molecular evolution ofphosphoenolpyruvate carboxylase. Plant, Cell andEnvironment, 17, 31-43[30]Turner W L, Knowles V L, Plaxton W C. 2005. Cytosolicpyruvate kinase: subunit composition, activity, andamount in developing castor and soybean seeds, andbiochemical characterization of the purified castor seedenzyme. Planta, 222, 1051-1062[31]Turpin D H, Weger H G. 1990. Interactions betweenphotosynthesis, respiration and nitrogen assimilation.In: Dennis D T, Turpin D H, eds., Plant Physiology,Biochemistry and Molecular Biology. LongmanScientific, Singapore. pp. 422-433[32]Uhrig R G, O’Leary B, Spang H E, MacDonald J A, She YM, Plaxton W C. 2008. Coimmunopurification ofphosphoryl a ted ba c t er i a l - and pl ant - typ ephosphoenolpyruvate carboxylases with the plastidialpyruvate dehydrogenase complex from developingcastor oil seeds. Plant Physiology, 146, 1346-1357[33]Vidal J, Chollet R. 1997. Regulatory phosphorylation of C4phosphoenolpyruvate carboxylase. Trends in PlantScience, 2, 230-237[34]Weber H, Borisjuk L, Wobus U. 2005. Molecular physiologyof legume seed development. Annual Review of PlantBiology, 56, 253-279[35]Yang M F, Xu Y N. 2007. Oleate accumulation, induced bysilencing of microsomal omega-6 desaturase, declineswith leaf expansion in transgenic tobacco[36]Journal ofPlant Physiology, 164, 23-30[37]Yu S L, Pan L J, Yang Q L, Chen M N, Zhang H S. 2010.Identification and expression analysis of thephosphoenolpyruvate carboxylase gene family in peanut(Arachis hypogaea L.). Agricultural Sciences in China,9, 477-487[38]Yu S L, Wang C T, Yang Q L, Zhang X D, Zhang X Y, CaoY L, Liang X Q, Liao B S, Yu LN,Wan Y S. 2011. PeanutGenetic Breeding in China. Shanghai Science andTechnology Press, China. pp. 18-32(in Chinese) |
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