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Journal of Integrative Agriculture  2026, Vol. 25 Issue (1): 394-397    DOI: 10.1016/j.jia.2025.08.001
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Point-of-care antigen detection for porcine deltacoronavirus: Colloidal gold and fluorescent immunochromatographic test strips

Zezhao Cao1*, Junchao Shi1*, Ruijie Hu1, Jun Xue1, Gaili Wang2, Zi Li1, Huabo Yu1, Wei Liu1, Wenqi He1, Hualei Wang1, Haili Zhang1#, Yungang Lan1#

1 State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases/Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China

2 Jilin Academy of Animal Husbandry and Veterinary Medicine, Changchun 130062, China

 Highlights 
By conjugating the same anti-N monoclonal antibody (mAb4-mAb1) with colloidal gold or fluorescent microspheres, this study developed two rapid point-of-care antigen immunochromatographic strips for the detection of porcine deltacoronavirus.
The fluorescent microsphere-based lateral flow test strip demonstrated a sensitivity of 101.7 TCID50/0.1 mL, which is fourfold higher than that of the colloidal gold-based assay.
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摘要  
猪德尔塔冠状病毒(PDCoV)感染可引起仔猪严重腹泻、脱水甚至死亡,威胁养猪业发展,且PDCoV具备跨种传播的潜能,存在不容忽视的人兽共患病风险。因此,开发PDCoV现场快速检测工具对其防控至关重要。本研究针对 PDCoV 高度保守的核衣壳(N)蛋白,建立了胶体金和荧光微球两种免疫层析试纸条。首先,原核表达系统表达并纯化获得N蛋白,免疫小鼠后采用杂交瘤技术筛选出 4 株高亲和力单克隆抗体。经配对优化,选择最优组合制备胶体金与荧光微球标记试纸,均可在 5 分钟内完成检测。胶体金试纸条对病毒感染细胞培养物的检测下限为 102.3 TCID50/0.1 mL,荧光试纸条灵敏度更高,达101.7 TCID50/0.1 mL。两种试纸条均高度特异,与猪血凝性脑脊髓炎病毒(PHEV)、伪狂犬病病毒(PRV)、猪圆环病毒 2 型(PCV2)、猪圆环病毒 3 型(PCV3)、塞内卡病毒(SVA)及牛疱疹病毒4型(BoHV-4)等均无交叉反应。两种试纸条在室温下可稳定保存 6 个月,有较好的稳定性。临床测试腹泻仔猪样本显示:胶体金试纸条与 qRT-PCR 的符合率为 90.32%(28/31),荧光试纸条与 qRT-PCR 的符合率为 96.77%(30/31)。结果表明,本研究建立的两种试纸条操作简便、快速,无需专业设备,肉眼或便携紫外灯即可判读结果,具有良好的临床应用潜力,为 PDCoV 现场筛查、疫情溯源及跨物种传播监测提供了重要技术支持。




Received: 06 March 2025   Accepted: 12 July 2025 Online: 05 August 2025  
Fund: 

This work was financially supported by the National Key Research and Development Program of China (2021YFF0703600).

About author:  Zezhao Cao, E-mail: 599142457@qq.com; Junchao Shi, E-mail: sjc@jlu.edu.cn; #Correspondence Haili Zhang, E-mail: zhanghaili@jlu.edu.cn; Yungang Lan, lanyungang@jlu.edu.cn * These authors contributed equally to this work.

Cite this article: 

Zezhao Cao, Junchao Shi, Ruijie Hu, Jun Xue, Gaili Wang, Zi Li, Huabo Yu, Wei Liu, Wenqi He, Hualei Wang, Haili Zhang, Yungang Lan. 2026. Point-of-care antigen detection for porcine deltacoronavirus: Colloidal gold and fluorescent immunochromatographic test strips. Journal of Integrative Agriculture, 25(1): 394-397.

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