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Journal of Integrative Agriculture  2026, Vol. 25 Issue (11): 4613-4620    DOI: 10.1016/j.jia.2025.06.006
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Functions and regulations of RNA-binding proteins in skeletal muscle development and meat production

Xiaoqin Liu1, 2, 3, 4, Gardner Graham2, Zhonglin Tang1, 3, 4, Calnan Honor2#, Yalan Yang1, 3, 4#

1 Kunpeng Institute of Modern Agriculture at Foshan, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Foshan 528226, China

2 College of Science, Health, Engineering and Education, Murdoch University, Murdoch, WA 6150, Australia

3 Key Laboratory of Livestock and Poultry Multi-Omics of Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China

4 Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China

 Highlights 
● Systematically review the functions and mechanisms of RNA-binding proteins (RBPs) in skeletal muscle development.
● Explore the potential breeding value of RBPs in the genetic improvement of meat production traits in livestock and poultry.
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摘要  

RNA结合蛋白(RBPs)在基因表达的转录及转录后调控中发挥重要作用。近年来,大量研究表明,RBPs是肌生成过程中的关键调控因子,为深入解析骨骼肌发育与生长的分子机制奠定了重要基础。然而,尽管RBPs在畜禽产肉性状调控方面展现出潜在的应用价值,但其在畜禽中的具体功能和作用机制仍需进一步研究。本综述系统总结了RBPs的基本特性,包括其两大主要分类方法、高度进化保守性以及组织特异性。随后,详细阐述了RBPs在骨骼肌生成中的功能与调控机制。这些RBPs可通过参与细胞核内的可变剪接和多聚腺苷酸化实现转录后调控;转运至胞质后,则通过介导胞质多聚腺苷酸化、维持mRNA稳定性、充当非编码RNA分子海绵或调控翻译效率等多种方式参与骨骼肌生成的调控。此外,部分RBPs还可通过动态核质穿梭和液-液相分离进一步调控骨骼肌生成。本文以hnRNPs、MBNL和RBFOX等三个家族为例,深入剖析了RBPs在骨骼肌生成中的作用,揭示其在肌细胞增殖、分化和融合等过程中的关键角色。最后,综述了11个对家畜产量量和肌纤维组成具有潜在调控作用的RBPs。其中,RPS27L基因与猪体重呈负相关,其余10个RNA结合蛋白分别在体外实验或小鼠模型中显示出对产肉性状的影响。此外,基于最新的PigGTEx数据库分析发现,包括IGF2BP2、METTL3在内的更多RBPs被预测与产肉性状显著相关。尽管已有综述探讨了RBPs在骨骼肌中的作用,但本文首次聚焦于RBPs在家畜瘦肉产量和肌纤维组成的调控,并探讨其在产肉性状改良中的潜在育种价值,旨在为改善家畜产肉性状的遗传改良提供更为全面和坚实的理论依据。



Abstract  

RNA-binding proteins (RBPs) predominantly regulate gene expression at both the transcriptional and post-transcriptional levels through multiple mechanisms, such as alternative splicing and alternative polyadenylation.  Increasing evidence indicates that RBPs are crucial regulators of myogenesis, providing a foundation for understanding the development and growth of skeletal muscle.  However, the role of RBPs in regulating meat production traits in livestock remains underexplored, despite their potential benefits to the meat industry.  In this review, we summarized the fundamental characteristics of RBPs, along with their functions and regulatory mechanisms in skeletal myogenesis.  We also highlighted the potential roles of RBPs for improving meat production traits, focusing on lean meat yield and myofiber composition in livestock.  Our aim is to deepen the understanding of how RBPs govern skeletal muscle development, contributing to the improvement of meat production traits in livestock and poultry.

Keywords:  RNA-binding proteins       skeletal myogenesis       meat production       lean meat yield       myofiber composition  
Received: 30 August 2024   Accepted: 26 May 2025 Online: 03 June 2025  
Fund: 

This work was supported by the National Key Scientific Research Projectof China (2023YFF1001100), the National Natural Science Foundation of China (U23A20229 and 32172697) and the Agricultural Science and Technology Innovation Program of China (CAAS-ZDRW202406).  

About author:  Xiaoqin Liu, E-mail: liuxiaoqin112@163.com; #Correspondence Calnan Honor, E-mail: honor.calnan@murdoch.edu.au; Yalan Yang, E-mail: yangyalan@caas.cn

Cite this article: 

Xiaoqin Liu, Gardner Graham, Zhonglin Tang, Calnan Honor, Yalan Yang. 2026. Functions and regulations of RNA-binding proteins in skeletal muscle development and meat production. Journal of Integrative Agriculture, 25(11): 4613-4620.

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