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Journal of Integrative Agriculture  2024, Vol. 23 Issue (9): 3104-3117    DOI: 10.1016/j.jia.2024.03.019
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Adipokinetic hormone signaling regulates adult dehydration resistance in the migratory locust
Xianliang Huang1, 2*, Dai Shi1*, Kai Deng1*, Shuzhen Jia1, Ding Ding3, Li Hou3, Bing Chen1, 2#
1 College of Life Sciences, Hebei University, Baoding 071002, China
2 Hebei Basic Science Center for Biotic Interaction, Hebei University, Baoding 071002, China
3 State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
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摘要  
全球气候变化导致干旱事件愈发严峻,从而严重威胁各种生物的生存;然而,关于生物抗旱性分子调控机制的研究较少。本研究对不同干旱(食物介导)环境下飞蝗成虫的生物学性状进行系统比较,发现脂动激素(AKH)信号通路对飞蝗成虫抗旱性具有重要调控作用。主要结果如下,干旱导致飞蝗成虫的失水率降低、体重损失率增加和寿命缩短。转录组分析显示,干旱选择系和对照系飞蝗存在组织特异性基因表达变异。特别重要的是,AKH基因表达受到干旱选择和干旱胁迫的显著诱导。进一步结合RNA干扰和海藻糖外源补充,发现AKH1、AKH2或脂动激素受体(AKHR)基因干扰显著加速干旱环境下飞蝗成虫的水分散失和死亡,外源补充海藻糖能够改善由AKHAKHR干扰引起的不利影响。该研究结果表明AKH/AKHR信号通路介导的海藻糖代谢对飞蝗抵抗干旱胁迫至关重要,促进了对生物抗旱性调控机制的理解。


Abstract  

Drought events have become more severe under climate change, and this can pose a major threat to the survival of various organisms.  The molecular mechanisms involved in dehydration resistance are not well known.  Here, adults of the migratory locust, Locusta migratoria, were subjected to food-mediated dehydration, and adipokinetic hormone (AKH) signaling was found to play a key role in regulating dehydration resistance.  Specifically, dehydration shortened the lifespan, increased the body weight loss, and reduced the water loss rate in adult locusts.  Global transcriptome profiles revealed variations in tissue-specific gene expression between dehydration-resistant locusts and normal locusts.  Importantly, dehydration selection and exposure induced prominent expression of AKH genes in the retrocerebral complex of adult locusts.  Furthermore, individual knockdown of AKH1, AKH2, or AKH receptor (AKHR) accelerated water loss and shortened the lifespan of adult locusts under dehydration conditions, and trehalose supplementation ameliorated the negative effects caused by interference with AKH or AKHR.  These findings demonstrated that AKH/AKHR signaling-dependent trehalose metabolism plays a crucial role in regulating locust dehydration resistance and thus provide novel insights into the regulatory mechanism underlying drought resistance.

Keywords:  adipokinetic hormone       adipokinetic hormone receptor       trehalose       dehydration resistance       Locusta migratoria  
Received: 17 November 2023   Accepted: 11 January 2024
Fund: This work was supported by the National Key Research and Development Program of China (2022YFD1400503), the National Natural Science Foundation of China (32102208) and the Hebei Natural Science Foundation, China (C2022201042, C2021201052 and C2023201075).

About author:  Xianliang Huang, E-mail: xlhuang2019@hbu.edu.cn; Dai Shi, E-mail: 772125734@qq.com; Kai Deng, E-mail: 270189870@qq.com; #Correspondence Bing Chen, E-mail: chenbing@hbu.edu.cn * These authors contributed equally to this study.

Cite this article: 

Xianliang Huang, Dai Shi, Kai Deng, Shuzhen Jia, Ding Ding, Li Hou, Bing Chen. 2024. Adipokinetic hormone signaling regulates adult dehydration resistance in the migratory locust. Journal of Integrative Agriculture, 23(9): 3104-3117.

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