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Journal of Integrative Agriculture  2023, Vol. 22 Issue (3): 935-944    DOI: 10.1016/j.jia.2023.02.008
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Melatonin treatment alleviates chilling injury in mango fruit 'Keitt' by modulating proline metabolism under chilling stress
Mariama KEBBEH, DONG Jing-xian, HUAN Chen, SHEN Shu-ling, LIU Yan, ZHENG Xiao-lin#
College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, P.R.China
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芒果在低温贮藏下常发生冷害(CI)。因此,迫切需要采用先进技术来解决芒果低温胁迫的伤害,提升产品的价值。本文研究了0.1 (MT1)和0.2 mmol L-1 (MT2)褪黑素溶液浸泡30分钟处理对‘凯特’芒果在低温藏期间CI、脯氨酸代谢和相关基因表达的影响。结果表明:与对照相比,MT1浓度褪黑素处理显著降低了芒果果实冷藏期间的CI发展,增加了脯氨酸含量;这些变化伴随着脯氨酸生物合成关键酶活性及其编码基因表达的增加,包括吡啶-5-羧酸合成酶(P5CS)、吡啶-5-羧酸还原酶(P5CR)、鸟氨酸D -氨基转移酶(OAT)、P5CS2P5CR2OAT3。同时,经MT1处理芒果的脯氨酸脱氢酶(PDH)活性和PDH3表达量低于对照。因此,褪黑素处理通过调控脯氨酸代谢导致脯氨酸的累积,进而有助于增强‘凯特’芒果果实的抗冷性。


Mangoes often suffer from low temperature-induced chilling injury (CI) during postharvest cold storage.  Therefore, advanced techniques are crucial and in high demand to solve the chilling stress of mango fruit for a higher value.  This study addresses chilling stress modulation by investigating the effects of melatonin treatment on CI, proline metabolism, and related gene expressions of ‘Keitt’ mango during cold storage after dipped in 0 (control), 0.1 (MT1), and 0.2 mmol L–1 (MT2) melatonin solution for 30 min.  The results revealed that melatonin treatment in MT1 significantly reduced CI development and increased proline content in mango fruit during cold storage compared to the control.  These changes were along with increases in the activity of critical enzymes as well as the expression of encoding genes involved in proline biosynthesis, such as pyrroline-5-carboxylate synthetase (P5CS), pyrroline-5-carboxylate reductase (P5CR), ornithine D-aminotransferase (OAT), P5CS2, P5CR2, and OAT3.  Additionally, proline dehydrogenase (PDH) activity and the expression of the PDH3 gene associated with proline dehydrogenation were lower in MT1-treated mangoes than the controlled group.  Thus, melatonin treatment has regulated proline metabolism resulting in the accumulation of proline, subsequently contributing to enhancing the chilling tolerance of ‘Keitt’ mango fruit.

Keywords:  chilling injury (CI)       cold storage        mango fruit        melatonin        proline metabolism  
Received: 24 June 2022   Accepted: 14 November 2022
Fund: This research project was financially supported by the National Natural Science Foundation of China (32072280).
About author:  #Correspondence ZHENG Xiao-lin, E-mail:

Cite this article: 

Mariama KEBBEH, DONG Jing-xian, HUAN Chen, SHEN Shu-ling, LIU Yan, ZHENG Xiao-lin. 2023. Melatonin treatment alleviates chilling injury in mango fruit 'Keitt' by modulating proline metabolism under chilling stress. Journal of Integrative Agriculture, 22(3): 935-944.

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