|Halloween genes AhCYP307A2 and AhCYP314A1 modulate last instar larva–pupa–adult transition, ovarian development and oogenesis in Agasicles hygrophila (Coleoptera: Chrysomelidae)
JIN Ji-su1*, LIU Yi-ran1*, ZHOU Zhong-shi1, WAN Fang-hao1, 2, GUO Jian-ying1#
1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2 Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, P.R.China
In insects, ecdysteroids are synthesized by genes of the Halloween family and play important roles in several key developmental events, including molting and metamorphosis. However, the roles of these genes in Agasicles hygrophila are still largely unknown. In this study, the expression patterns of the two Halloween genes AhCYP307A2 and AhCYP314A1 were determined by quantitative PCR (qPCR) at different developmental stages. Moreover, the functions of these two genes were explored using RNA interference (RNAi), and ovarian development was observed by dissecting the ovaries of A. hygrophila females. The qPCR results showed that AhCYP307A2 and AhCYP314A1 were highly expressed in last instar larvae and in adult females. In addition, AhCYP307A2 was also highly expressed in eggs and pupae but was markedly lower than in third-instar larvae and females. The RNAi results showed that the injection of dsAhCYP307A2 or dsAhCYP314A1 markedly inhibited their expression and the transcription levels of three related AhVgs. Knockdown of AhCYP307A2 or AhCYP314A1 significantly inhibited larval molting, impaired last instar larva–pupa–adult transition, delayed ovarian development, and stopped egg production (i.e., no eggs were laid). These results indicate that AhCYP307A2 and AhCYP314A1 play important regulatory roles in last instar larva–pupa–adult transition and reproduction in A. hygrophila.
Received: 25 January 2022
Accepted: 09 May 2022
This work was supported by the National Natural Science Foundation of China (31572068 and 32172486).
|About author: JIN Ji-su, E-mail: email@example.com; LIU Yi-ran, E-mail: firstname.lastname@example.org; #Correspondence GUO Jian-ying, Tel: +86-10-82109572, E-mail: email@example.com
* These authors contributed equally to this study.
Cite this article:
JIN Ji-su, LIU Yi-ran, ZHOU Zhong-shi, WAN Fang-hao, GUO Jian-ying.
Halloween genes AhCYP307A2 and AhCYP314A1 modulate last instar larva–pupa–adult transition, ovarian development and oogenesis in Agasicles hygrophila (Coleoptera: Chrysomelidae). Journal of Integrative Agriculture, 22(3): 812-824.
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