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Journal of Integrative Agriculture  2022, Vol. 21 Issue (8): 2253-2263    DOI: 10.1016/S2095-3119(21)63874-1
Special Issue: 园艺-分子生物合辑Horticulture — Genetics · Breeding
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Identification of soft rot resistance loci in Brassica rapa with SNP markers
LIU Meng-yang*, WU Fang*, GE Yun-jia*, LU Yin, ZHANG Xiao-meng, WANG Yan-hua, WANG Yang, YAN Jing-hui, SHEN Shu-xing, ZHAO Jian-jun, MA Wei
State Key Laboratory of North China Crop Improvement and Regulation, Ministry of Science and Technology/Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei/Collaborative Innovation Center of Vegetable Industry in Hebei/College of Horticulture, Hebei Agricultural University, Baoding 071000, P.R.China
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为了研究软腐病的抗性基因,我们筛选出了一份易感软腐病大白菜A03、一份抗软腐病小白菜华冠以及一份抗软腐病突变体sr。本研究以感病大白菜A03与抗软腐病小白菜华冠为亲本进行杂交,获得F2代分离群体来定位大白菜抗软腐病数量性状位点(QTLs)。利用构建的高密度遗传图谱检测到3个QTL位点,共包含166个基因。基于已有的转录组数据,在大白菜受Pc侵染的重要防御调控期,我们对这166个基因在感病大白菜A03和抗病突变体sr内的表达量进行了分析,共筛选出6个候选基因与白菜软腐病防御反应相关。其中,基因TIFY10B (JAZ2BraA07g038660.3C) 位于A07连锁群的主效QTL位点DRQTL-3上,推测可能是白菜软腐病防御机制中起主效作用的关键基因之一。本研究为进一步研究白菜类作物中软腐病抗性机理奠定了基础。


Soft rot caused by Pectobacterium carotovorum (Pc) is a devastating disease of Brassica rapa, causing substantial reductions in crop yield and quality.  Identifying genes related to soft rot resistance is the key to solving this problem.  To characterize soft rot resistance, we screened a soft rot-susceptible Chinese cabbage (A03), a resistant pakchoi (‘Huaguan’), and a resistant mutant (sr).  An F2 population was generated by crossing susceptible Chinese cabbage A03 and resistant pakchoi ‘Huaguan’ to identify quantitative trait loci (QTLs) that confer soft rot resistance.  A high-density genetic map was constructed and the three QTLs identified contain 166 genes.  Based on available transcriptome data, we analyzed the expression of the 166 genes during an important defense regulatory period in Pc infection in both A03 and the resistant mutant sr.  Among the 166 genes, six candidate genes were related to the soft rot defense response in Brapa.  TIFY10B (JAZ2, BraA07g038660.3C) was located in the major soft rot resistance QTL, DRQTL-3 on A07, and we speculate that this gene may play an important role in the defense mechanism against soft rot in Brapa.  This study lays the foundation for further investigations on the mechanism of soft rot resistance in Brapa crops.

Keywords:  soft rot       quantitative trait loci-sequencing        Brassica rapa  
Received: 04 August 2021   Accepted: 23 November 2021
Fund: The authors are grateful to Prof. Xie Hua (Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, China) for the gift of the Pc pathogen.  This work was supported by the National Natural Science Foundation of China (31672151 and 31902005), the China Postdoctoral Science Foundation (2019M651059), the Natural Science Foundation of Hebei, China (C2020204111), the International Cooperation Project in the Science and Technology Support Program of Hebei, China (2019YX023A), the International Cooperation Base Project in the Technology of Hebei, China (20592901D), 100 Foreign Experts Plan of Hebei Province and the Starting Grant from Hebei Agricultural University, China (YJ201955), the Science and Technology Research Project of Universities in Hebei Province, China (QN2021074).

About author:  LIU Meng-yang, E-mail:; WU Fang, E-mail:; GE Yun-jia, E-mail:; Correspondence MA Wei, E-mail: * These authors contributed equally to this study.

Cite this article: 

LIU Meng-yang, WU Fang, GE Yun-jia, LU Yin, ZHANG Xiao-meng, WANG Yan-hua, WANG Yang, YAN Jing-hui, SHEN Shu-xing, ZHAO Jian-jun, MA Wei. 2022. Identification of soft rot resistance loci in Brassica rapa with SNP markers. Journal of Integrative Agriculture, 21(8): 2253-2263.

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