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Journal of Integrative Agriculture  2022, Vol. 21 Issue (2): 578-585    DOI: 10.1016/S2095-3119(20)63471-2
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Waxy allele diversity in waxy maize landraces of Yunnan Province, China
WU Xiao-yang, LONG Wen-jie, CHEN Dan, ZHOU Guo-yan, DU Juan, WU Shao-yun, CAI Qing
Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, P.R.China

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糯玉米是主要的鲜食玉米类型之一,waxy基因突变导致了玉米籽粒的糯性。中国糯玉米地方品种丰富,云南及其周边地区被认为是糯玉米的起源中心和遗传多样性中心。已知中国糯玉米waxy等位基因包括wx-D7wx-D10wx-Cin4wx-124wx-Reinawx-xuanwei。这些等位基因的突变位点都发生在waxy基因的编码区,而调控区引起waxy基因的突变在玉米中却鲜有报道。本研究405份云南糯玉米地方品种为材料,通过分子标记检测,鉴定waxy基因上游3.5Kb调控区的大片段插入和缺失。研究发现三类不同的waxy等位基因wx-PIF/Harbingerwx-hATwx-Elote2三类等位基因分别属于304bpPIF/Harbinger家族 MITE类转座子的插入、560bphAT家族MITE类转座子的插入和6560bpLTR-like转座子的插入。此外研究中完成了云南70%以上的糯玉米地方品种waxy等位基因鉴定,为糯玉米地方品种的利用提供基础

Abstract  Waxy maize is one of the main fresh-eating maize types, and a mutation of the waxy gene causes the waxy character of maize grains.  China is rich in waxy maize landraces, and Yunnan and its surrounding areas, are the place of origin and genetic diversity center of Chinese waxy maize.  The six known waxy alleles of Chinese waxy maize are wx-D7, wx-D10, wx-Cin4, wx-124, wx-Reina, and wx-Xuanwei.  The mutation sites of these alleles all occur in the coding region of the waxy gene, however, the mechanism by which the waxy characteristic is caused by the mutation in the regulatory region has only been reported rarely in maize.  In this study, 405 waxy maize landraces from Yunnan were used as materials to identify the insertion and deletion of a large sequence fragment in the upstream ~3.5 kb regulatory region of the waxy gene by molecular marker detection.  Three different waxy alleles were identifed in this study: wx-PIF/Harbinger, wx-hAT and wxElote2.  These three types of mutations all represented transposons inserted into the regulatory region of the waxy gene.  Wx-PIF/Harbinger was a 304-bp MITE class transposon insertion belonging to the PIF/Harbinger family, while wx-hAT was a 560-bp MITE class transposon insertion belonging to the hAT family, and wx-Elote2 was a 6 560-bp LTR-like transposon insertion.  In this study, the alleles were identifed for more than 70% of the waxy maize landraces in Yunnan, which provids a basis for the utilization of these waxy maize landraces.
Keywords:  waxy maize landraces       Waxy allele        transposon        gene regulatory region        Yunnan  
Received: 20 April 2020   Accepted: 28 October 2020
Fund: This study was supported by the National Crop Sharing and Service Platform-Yunnan subPlatform, China (NICGR2018-030) and the Post-doctoral Targeted Funding of Yunnan Province, China (YRST 2018[168]).
About author:  Received 20 April, 2020 Accepted 28 October, 2020 WU Xiao-yang, E-mail:; Correspondence CAI Qing, Tel: +86-871-65123762, Fax: +86-871-65144977, E-mail:

Cite this article: 

WU Xiao-yang, LONG Wen-jie, CHEN Dan, ZHOU Guo-yan, DU Juan, WU Shao-yun, CAI Qing. 2022. Waxy allele diversity in waxy maize landraces of Yunnan Province, China. Journal of Integrative Agriculture, 21(2): 578-585.

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