Abstract Skeletal muscle development is closely related with the amount of meat production and its quality in chickens. Natriuretic peptides (NPs) play an important role in myotube formation and fat oxidation of skeletal muscle in animals. The effect of C-type natriuretic peptide (CNP), an important member of the NPs, and its underlying molecular mechanisms in skeletal muscle are incompletely understood. Treatment of myoblasts with CNP led to enhanced proliferation/differentiation and significantly upregulated (P<0.05) mRNA expression of the CNP receptors natriuretic peptide receptor B (NPRB) and the clearance receptor C (NPRC). In cells exposed to CNP, 142 differentially expressed genes (84 up-regulation and 58 down-regulation) (P<0.05) were identified by RNA-sequencing compared with those in control cells. Sixteen genes were significantly enriched (P<0.05) in the metabolic pathway, and six of them (phospholipase C β4, phospholipase C β2, phosphoglycerate mutase 1, creatine kinase B, peroxiredoxin 6 and CD38) were closely related to skeletal muscle development and differentially expressed. In conclusion, CNP stimulated differentiation of myoblasts by upregulating expression of the NPRB and NPRC receptors and enriching key genes in the metabolic pathway.
Received: 02 September 2020
Accepted: 03 April 2021
Fund: The research was supported by the Natural Science Foundation of Jiangsu Province, China (BK20191217), the Independent Innovation Fund Project of Agricultural Science and Technology of Jiangsu (CX(20)2012), the China Agriculture Research System of MOF and MARA (CARS-41-Z05), the Key Laboratory of Poultry Genetics and Breeding in Jiangsu (JQLAB-ZZ-201705 and JQLAB-ZZ-202008), and the Special Funds for Transformation of Scientific and Technological Projects in Jiangsu Province (BA2019049).
About author: Huang Hua-yun, E-mail: huanghuayun520@163.com; Correspondence Zhao Zhen-hua, Tel/Fax: +86-514-85599073, E-mail: zzh0514@163.com
* These authors contributed equally to this study.
HUANG Hua-yun, LIANG Zhong, LIU Long-zhou, LI Chun-miao, HUANG Zhen-yang, WANG Qian-bao, LI Shou-feng, ZHAO Zhen-hua.
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