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Journal of Integrative Agriculture  2021, Vol. 20 Issue (12): 3299-3311    DOI: 10.1016/S2095-3119(20)63462-1
Special Issue: 农业生态环境-土壤微生物合辑Agro-ecosystem & Environment—Soil microbe
Agro-ecosystem & Environment Advanced Online Publication | Current Issue | Archive | Adv Search |
Assembly and co-occurrence patterns of rare and abundant bacterial sub-communities in rice rhizosphere soil under short-term nitrogen deep placement
LI Gui-long1, 2, WU Meng1, 2, LI Peng-fa1, 2, WEI Shi-ping1, 2, LIU Jia1, 3, JIANG Chun-yu1, LIU Ming1, 2, LI Zhong-pei1, 2 
1 State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, P.R.China
2 University of Chinese Academy of Sciences, Beijing 100049, P.R.China
3 Soil and Fertilizer & Resources and Environment Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, P.R.China
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Nitrogen (N) deep placement has been found to reduce N leaching and increase N use efficiency in paddy fields.  However, relatively little is known how bacterial consortia, especially abundant and rare taxa, respond to N deep placement, which is critical for understanding the biodiversity and function of agricultural ecosystem.  In this study, Illumina sequencing and ecological models were conducted to examine the diversity patterns and underlying assembly mechanisms of abundant and rare taxa in rice rhizosphere soil under different N fertilization regimes at four rice growth stages in paddy fields.  The results showed that abundant and rare bacteria had distinct distribution patterns in rhizosphere samples.  Abundant bacteria showed ubiquitous distribution; while rare taxa exhibited uneven distribution across all samples.  Stochastic processes dominated community assembly of both abundant and rare bacteria, with dispersal limitation playing a more vital role in abundant bacteria, and undominated processes playing a more important role in rare bacteria.  The N deep placement was associated with a greater influence of dispersal limitation than the broadcast N fertilizer (BN) and no N fertilizer (NN) treatments in abundant and rare taxa of rhizosphere soil; while greater contributions from homogenizing dispersal were observed for BN and NN in rare taxa.  Network analysis indicated that abundant taxa with closer relationships were  usually more likely to occupy the central position of the network than rare taxa.  Nevertheless, most of the keystone species were rare taxa and might have played essential roles in maintaining the network stability.  Overall, these findings highlighted that the ecological mechanisms and co-occurrence patterns of abundant and rare bacteria in rhizosphere soil under N deep placement.
Keywords:  rare bacteria        community assembly        network analysis        co-occurrence patterns        N deep placement  
Received: 20 July 2020   Accepted:
Fund: This work was supported by the National Key Research and Development Program of China (2016YFD0200309 and 2018YFD0301104-01).
Corresponding Authors:  Correspondence LI Zhong-pei, Tel: +86-25-86881323, E-mail:   
About author:  LI Gui-long, Mobile: +86-13455171603, E-mail:;

Cite this article: 

LI Gui-long, WU Meng, LI Peng-fa, WEI Shi-ping, LIU Jia, JIANG Chun-yu, LIU Ming, LI Zhong-pei. 2021. Assembly and co-occurrence patterns of rare and abundant bacterial sub-communities in rice rhizosphere soil under short-term nitrogen deep placement. Journal of Integrative Agriculture, 20(12): 3299-3311.

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