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| Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells |
| ZHANG Zhi-bang*, XU Lei*, WEN Xue-xia, DONG Jian-guo, ZHOU Lei, GE Xin-na, YANG Han-chun, GUO Xin |
| Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University/State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China |
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Abstract The nonstructural protein 10 (nsp10) of porcine reproductive and respiratory syndrome virus (PRRSV) encodes for helicase which plays a vital role in viral replication. In the present study, a truncated form of nsp10, termed nsp10a, was found in PRRSV-infected cells and the production of nsp10a was strain-specific. Mass spectrometric analysis and deletion mutagenesis indicated that nsp10a may be short of about 70 amino acids in the N terminus of nsp10. Further studies by rescuing recombinant viruses showed that the Glu-69 in nsp10 was the key amino acid for nsp10a production. Finally, we demonstrated that nsp10a exerted little influence on the growth kinetics of PRRSV in vitro.
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Received: 13 December 2017
Accepted:
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| Fund: This work was supported by the National Key Technology R&D Program of China (2015BAD12B01-2), the Major Program of National Natural Science Foundation of China (31490603) and the earmarked fund for Modern Agro-industry Technology Research System of China (CARS-36). |
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Corresponding Authors:
Correspondence YANG Han-chun, Tel/Fax: +86-10-62731296, E-mail: yanghanchun1 @cau.edu.cn; GUO Xin, Tel/Fax: +86-10-62732875, E-mail: guoxincau@cau.edu.cn
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| About author: ZHANG Zhi-bang, Tel: +86-10-62732875, E-mail: zhibangzhang @126.com; XU Lei, E-mail: 358736128@qq.com; * These authors contributed equally to this study.
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Cite this article:
ZHANG Zhi-bang, XU Lei, WEN Xue-xia, DONG Jian-guo, ZHOU Lei, GE Xin-na, YANG Han-chun, GUO Xin.
2018.
Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells. Journal of Integrative Agriculture, 17(05): 1171-1180.
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