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| Research on the appropriate way to transfer exogenous substances into chicken embryos |
| WANG Yi-lin*, JIN Kai*, HE Na-na, CHENG Shao-ze, ZUO Qi-sheng, LI Dong, WANG Ying-jie, WANG Fei, JI Yan-qing, LU Zhen-yu, ZHANG Chen, WANG Man, ZHAO Rui-feng, YU Xin-jian, ZHANG Ya-ni, ZHAO Wen-ming, LI Bi-chun |
| College of Animal Science and Technology/Jiangsu Province Key Laboratory of Animal Breeding and Molecular Design, Yangzhou University, Yangzhou 225009, P.R.China |
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Abstract In biological research, chicken embryos are a classic experimental model for the exploration of the embryonic development and cell differentiation. Transferring exogenous substances into chicken embryos for producing medical antibodies has been widely used in the production practice. However, there are few studies about the effect of the different injection site and dosage on chicken embryos. The aim of this study was to explore the effects of different injection sites and dosages on chicken embryo hatching rate and development, so as to provide a basis for further studies using the chicken embryo model. Freshly laid eggs (Rugao yellow chicken) were injected with different doses of saline at the tip, equatorial plane and the blunt end of the egg shell, respectively. Egg hatching rate was recorded and compared among injection sites and different doses. A trypan blue stain was also injected at the aforementioned sites and the growth of chicken embryos was observed. The SPSS (statistical package for the social science) software was used to analyze the relationship between the chicken eggs hatching rate and the different injection sites or the different dosages. The experimental results showed that there were significant differences on egg hatching rates among the different injection sites and doses (P<0.05). The hatchability of the blunt end injection group was significantly higher than that of the other two sites. The egg hatching rate decreased with increased saline doses. The egg hatching rate of the 100 µL saline injection group was higher than the 200 and 300 µL dosage groups. Ultimately, we suggest that the optimal chicken embryo injection process is during early development, at the blunt end site with a dose less than 100 µL to minimize damage to the egg.
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Received: 09 November 2016
Accepted:
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| Fund: This work was supported by the National Natural Science Foundation of China (31472087), the Research and Innovation Program for Graduate Cultivation of Jiangsu Proviance, China in 2010. |
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Corresponding Authors:
Correspondence LI Bi-chun, Tel: +86-514-87977207, Fax: +86-514-87350440, E-mail: yubcli@yzu.edu.cn
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| About author: WANG Yi-lin, Mobile: +86-18252713425, Fax: +86-514-87350440, E-mail: elainewang0227@163.com; JIN Kai, Mobile: +86-18252715867, Fax: +86-514-87350440, E-mail: 786282373@qq.com; |
Cite this article:
WANG Yi-lin, JIN Kai, HE Na-na, CHENG Shao-ze, ZUO Qi-sheng, LI Dong, WANG Ying-jie, WANG Fei, JI Yan-qing, LU Zhen-yu, ZHANG Chen, WANG Man, ZHAO Rui-feng, YU Xin-jian, ZHANG Ya-ni, ZHAO Wen-ming....
2017.
Research on the appropriate way to transfer exogenous substances into chicken embryos. Journal of Integrative Agriculture, 16(10): 2257-2263.
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