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| Screening of genes of secreting acetic acid from Aspergillus niger H1 to improve phosphate solubilization |
| LIU Xue1, 2, ZHU Chang-xiong1, Frank Delvigne2, GONG Ming-bo3 |
1 Institute of Agricultural Environment and Sustainable Development, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 Université de Liège, Gembloux Agro-Bio Tech, Unité de Bio-Industries, Gembloux 5030, Belgium
3 Key Laboratory of Microbial Resources, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China |
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Abstract A primary cDNA library of Aspergillus niger H1 was constructed using the switching mechanism at the 5´ end of the RNA transcript (SMART) technique. A total of 169 clones exhibited halos when grown on tricalcium phosphate medium, and the H-46 clone displayed a clear halo. The full-length cDNA of the clone H-46 clone was 1 407 bp in length with a complete open reading frame (ORF) of 816 bp, and it encoded a protein that contained 272 amino acids. Multiple alignment analysis revealed a high degree of homology between the ORFs of the H-46 clone and the Bax inhibitor family (BI-1-like) proteins of other fungi. Acetic acid was secreted by Escherichia coli DH5α that express the BI-1-like gene. The level attained was 492.52 mg L−1, which was associated with the release of 0.212 mg mL−1 of soluble phosphate at 28 h. These results showed that the heterologous expression of BI-1-like genes in Eschericha coli DH5α increased the secretion of acetic acid by altering the membrane permeability and enhancing the solubility of phosphate (P).
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Received: 21 June 2016
Accepted:
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| Fund: This work was supported by the National Natural Science Foundation of China (41440008). |
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Corresponding Authors:
Correspondence GONG Ming-bo, Tel: +86-10-82108683, E-mail: gongcaas@163.com
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| About author: LIU Xue, Tel: +86-10-82108683, E-mail: liuxue@caas.cn; |
Cite this article:
LIU Xue, ZHU Chang-xiong, Frank Delvigne, GONG Ming-bo.
2017.
Screening of genes of secreting acetic acid from Aspergillus niger H1 to improve phosphate solubilization. Journal of Integrative Agriculture, 16(09): 2110-2114.
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