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| Role of duck plague virus glycoprotein C in viral adsorption: Absence of specific interactions with cell surface heparan sulfate |
| JING Yan-chun1, 2, 3*, WU Ying1, 2, 3*, SUN Kun-feng1, 2, 3, WANG Ming-shu1, 2, 3, CHENG An-chun1, 2, 3, CHEN Shun1, 2, 3, JIA Ren-yong1, 2, 3, ZHU De-kang2, 3, LIU Ma-feng1, 2, 3, YANG Qiao1, 2, 3, JING Bo2, CHEN Xiao-yue2, 3 |
1 Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China
2 Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu 611130, P.R.China
3 Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China |
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Abstract Many mammalian herpes viruses utilize heparan sulfate (HS) moieties present on cell surface proteoglycans as receptors for cell entry, and this process also requires viral glycoprotein C (gC) homologues. However, our understanding of the role of gC in facilitating attachment of other alpha-herpes viruses such as the duck plague virus (DPV) remains preliminary. To study the role of gC during DPV infection, we used a gC-deleted mutant virus (DPV-ΔgC-EGFP). Examination of the viral copy number by real-time PCR, as well as time course studies of viral adsorption and proliferation revealed that gC was involved in the viral binding to the cell surface. The affinity of viral glycoproteins (gB-DPV, gC-DPV, and gE-DPV) to HS was assessed using a prokaryotic expression system and HiTrapTM Heparin HP column chromatography. In addition, to confirm that gC played a role in the interaction between DPV and HS, viruses were treated with the HS analogue heparin and host cells were treated with its inhibitors heparinase prior to exposure to DPV-ΔgC-EGFP or wild-type strain Chinese virulent duck plague virus (DPV-CHv). The effects of heparin and heparinase on virus infectivity demonstrated that function of gC on viral adsorption is independent of interactions between gC and heparin sulfate on cell surface. All in all, this study demonstrated that the gC of DPV can mediate viral adsorption in an HS-independent manner, which distinguish it from the gC of some other alpha-herpes viruses. Future studies will be required to identify the receptors involved in gC protein binding to cells. This work provides us a foundation for further studies of examining the roles of gC in the adsorption during duck plague virus infection.
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Received: 05 April 2016
Accepted:
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| Fund: This work was supported by the grants from the National Natural Science Foundation of China (31072157), the National Key Technologies R&D Program of China during the 12th Five-Year Plan period (2015BAD12B05), the Foundation of China Agricultural Research System (CARS-43-8), and the Major Project of Education Department in Sichuan, China (16ZA0027). |
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Corresponding Authors:
CHENG An-chun, E-mail: chenganchun@vip.163.com; WANG Ming-shu, E-mail: mshwang@163.com
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| About author: JING Yan-chun, E-mail: 1140603804@qq.com |
Cite this article:
JING Yan-chun, WU Ying, SUN Kun-feng, WANG Ming-shu, CHENG An-chun, CHEN Shun, JIA Ren-yong, ZHU De-kang, LIU Ma-feng, YANG Qiao, JING Bo, CHEN Xiao-yue.
2017.
Role of duck plague virus glycoprotein C in viral adsorption: Absence of specific interactions with cell surface heparan sulfate. Journal of Integrative Agriculture, 16(05): 1145-1152.
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