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| Establishment of an avian leukosis virus subgroup A-resistant cell line |
| FENG Min1, 2, 3, 4*, DAI Man-man1, 2, 3, 4*, LIAO Ming1, 2, 3, 4, CAO Wei-sheng1, 2, 3, 4 |
1 College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, P.R.China
2 National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou 510642, P.R.China
3 Key Laboratory of Veterinary Vaccine Innovation, Ministry of Agriculture, Guangzhou 510642, P.R.China
4 Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, P.R.China |
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Abstract Rapid diagnostic methods for classifying avian leukosis subgroups in the field were needed for routine, large-scale screening. As a first step in method development, we inserted the avian leukosis virus subgroup A (ALV-A) env gene into plasmid pcDNA3.1/Zeo (+) and used this construct to transfect DF-1 cells. Zeocin-resistant cells were obtained after 2 weeks of zeocin selection. Then, the cells were analyzed using PCR, immunofluorescence, and Western blot for expression of the envA-encoded envelope protein after 30 serial passages. The DF-1/A cell line was completely resistant to 104 TCID50/0.1 mL (50% tissue culture infective dose) ALV-A and was partially resistant to 105 TCID50/0.1 mL ALV-A viral particles. By comparing the DF-1/A and DF-1 cell lines, an ALV-A isolate was identified using a gag-specific ELISA for capsid protein p27. Thus, we established a DF-1/A cell line that was resistant to ALV-A infection. This cell line will be useful as a diagnostic tool.
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Received: 13 April 2016
Accepted:
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| Fund: The work was founded by the National Key R&D Program of China (2016YFD0501606), the Public Industry Research Program, the Ministry of Agriculture of China (201203055), the Program of Science and Technology Development of Guangdong Province, China (2015A020209145), the China Meat-Type Chicken Research System (CARS-42-G09) and the Modern Agriculture Talents Support Program, Ministry of Agriculture of China ([2012] no.160). |
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Corresponding Authors:
CAO Wei-sheng, Tel: +86-20-85282536, Fax: +86-20-85280234, E-mail: caoweish@scau.edu.cn
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| About author: FENG Min, E-mail: hunanfengmin@163.com |
Cite this article:
FENG Min, DAI Man-man, LIAO Ming, CAO Wei-sheng.
2017.
Establishment of an avian leukosis virus subgroup A-resistant cell line. Journal of Integrative Agriculture, 16(04): 930-936.
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| Adkins H B, Brojatsch J, Naughton J, Rolls M M, Pesola J M, Young J A. 1997. Identification of a cellular receptor for subgroup E avian leukosis virus. Proceedings of the National Academy of Sciences of the United States of America, 94, 11617–11622.Bates P, Rong L, Varmus H E, Young J A, Crittenden L B. 1998. Genetic mapping of the cloned subgroup A avian sarcoma and leukosis virus receptor gene to the TVA locus. Journal of Virology, 72, 2505–2508.Bates P, Young J A, Varmus H E. 1993. A receptor for subgroup A Rous sarcoma virus is related to the low density lipoprotein receptor. Cell, 74, 1043–1051.Brojatsch J, Naughton J, Rolls M M, Zingler K, Young J A. 1996. CAR1, a TNFR-related protein, is a cellular receptor for cytopathic avian leukosis-sarcoma viruses and mediates apoptosis. Cell, 87, 845–855.Chai N, Bates P. 2006. Na+/H+ exchanger type 1 is a receptor for pathogenic subgroup J avian leukosis virus. Proceedings of the National Academy of Sciences of the United States of America, 103, 5531–5536.Coffin J M, Hughes S H, Varmus H E. 1997. The interactions of retroviruses and their hosts, In: Coffin J M, Hughes S H, Varmus H E, eds., Retroviruses. Cold Spring, Harbor, NY.Crittenden L B, Salter D W, Federspiel M J. 1989. Segregation, viral phenotype, and proviral structure of 23 avian leukosis virus inserts in the germ line of chickens. Theoretical and Applied Genetics, 77, 505–515. Crittenden L B, Stone H A, Reamer R H, Okazaki W. 1967. Two loci controlling genetic cellular resistance to avian leukosis-sarcoma viruses. Journal of Virology, 1, 898–904.Cui N, Su S, Chen Z, Zhao X, Cui Z. 2014. Genomic sequence analysis and biological characteristics of a rescued clone of avian leukosis virus strain JS11C1, isolated from indigenous chickens. The Journal of General Virology, 95, 2512–2522.Elleder D, Stepanets V, Melder D C, Senigl F, Geryk J, Pajer P, Plachy J, Hejnar J, Svoboda J, Federspiel M J. 2005. The receptor for the subgroup C avian sarcoma and leukosis viruses, Tvc, is related to mammalian butyrophilins, members of the immunoglobulin superfamily. Journal of Virology, 79, 10408–10419.Fenton S P, Reddy M R, Bagust T J. 2005. Single and concurrent avian leukosis virus infections with avian leukosis virus-J and avian leukosis virus-A in Australian meat-type chickens. Avian Pathology, 34, 48–54.Himly M, Foster D N, Bottoli I, Iacovoni J S, Vogt P K. 1998. The DF-1 chicken fibroblast cell line: Transformation induced by diverse oncogenes and cell death resulting from infection by avian leukosis viruses. Virology, 248, 295–304.Hunt H D, Lee L F, Foster D, Silva R F, Fadly A M. 1999. A genetically engineered cell line resistant to subgroup J avian leukosis virus infection (C/J). Virology, 264, 205–210.Ishizaki R, Vogt P K. 1966. Immunological relationships among envelope antigens of avian tumor viruses. Virology, 30, 375–387.Kozak M. 1991. An analysis of vertebrate mRNA sequences: Intimations of translational control. The Journal of Cell Biology, 115, 887–903.Lai H, Zhang H, Ning Z, Chen R, Zhang W, Qing A, Xin C, Yu K, Cao W, Liao M. 2011. Isolation and characterization of emerging subgroup J avian leukosis virus associated with hemangioma in egg-type chickens. Veterinary Microbiology, 151, 275–283.Li D, Qin L, Gao H, Yang B, Liu W, Qi X, Wang Y, Zeng X, Liu S, Wang X, Gao Y. 2013. Avian leukosis virus subgroup A and B infection in wild birds of Northeast China. Veterinary Microbiology, 163, 257–263.Li Y, Liu X, Liu H, Xu C, Liao Y, Wu X, Cao W, Liao M. 2013. Isolation, identification, and phylogenetic analysis of two avian leukosis virus subgroup J strains associated with hemangioma and myeloid leukosis. Veterinary Microbiology, 166, 356–364.Mothes W, Boerger A L, Narayan S, Cunningham J M, Young J A. 2000. Retroviral entry mediated by receptor priming and low pH triggering of an envelope glycoprotein. Cell, 103, 679–689.Payne L N, Biggs P M. 1965. Effect of lymphoid leukosis virus on the response of the chorioallantoic membrane to Rous sarcoma virus. Virology, 27, 620–623.Payne L N, Nair V. 2012. The long view: 40 years of avian leukosis research. Avian Pathology, 41, 11–19.Qin L, Gao Y, Ni W, Sun M, Wang Y, Yin C, Qi X, Gao H, Wang X. 2013. Development and application of real-time PCR for detection of subgroup J avian leukosis virus. Journal of Clinical Microbiology, 51, 149–154.Salter D W, Crittenden L B. 1989. Artificial insertion of a dominant gene for resistance to avian leukosis virus into the germ line of the chicken. Theoretical and Applied Genetics, 77, 457–461. Salter D W, Crittenden L B. 1991. Insertion of a disease resistance gene into the chicken germline. Biotechnology, 16, 125–131.Silva R F, Fadly A M, Taylor S P. 2007. Development of a polymerase chain reaction to differentiate avian leukosis virus (ALV) subgroups: Detection of an ALV contaminant in commercial Marek’s disease vaccines. Avian Diseases, 51, 663–667.Smith L M, Brown S R, Howes K, McLeod S, Arshad S S, Barron G S, Venugopal K, McKay J C, Payne L N. 1998. Development and application of polymerase chain reaction (PCR) tests for the detection of subgroup J avian leukosis virus. Virus Research, 54, 87–98.Venugopal K, Smith L M, Howes K, Payne LN. 1998. Antigenic variants of J subgroup avian leukosis virus: sequence analysis reveals multiple changes in the env gene. The Journal of General Virology, 79, 757–766.Vogt P K, Ishizaki R. 1965. Reciprocal patterns of genetic resistance to avian tumor viruses in two lines of chickens. Virology, 26, 664–672.Vogt P K, Ishizaki R. 1966. Patterns of viral interference in the avian leukosis and sarcoma complex. Virology, 30, 368–374.Zhang H N, Lai H Z, Qi Y, Zhang X T, Ning Z Y, Luo K J, Xin C A, Cao W S, Liao M. 2011. An ALV-J isolate is responsible for spontaneous haemangiomas in layer chickens in China. Avian Pathology, 40, 261–267.Zhang X, Liao M, Jiao P, Luo K, Zhang H, Ren T, Zhang G, Xu C, Xin C, Cao W. 2010. Development of a loop-mediated isothermal amplification assay for rapid detection of subgroup J avian leukosis virus. Journal of Clinical Microbiology, 48, 2116–2121. |
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