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Journal of Integrative Agriculture  2026, Vol. 25 Issue (10): 4126-4141    DOI: 10.1016/j.jia.2025.05.025
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Organic fertilizer substitution enhances maize yield and quality under reduced irrigation

Feier Wang1, Yao Guo2, Pan Li1, Xiayu Wu1, Hailong Qiu1, Wen Yin1#, Lianhao Zhao1, Zhilong Fan1, Falong Hu1, Wei He1, Hong Fan1, Qiang Chai1

1 State Key Laboratory of Arid Land Crop Science/College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China

2 College of Life Sciences, Northwest Normal University, Lanzhou 730070, China

 Highlights 
● A 20% reduction in irrigation combined with organic fertilizer substitution for 25% of chemical nitrogen (I1F2) enhanced maize yield and quality.
● I1F2 improved maize yield and quality by regulating the photo-physiological traits and nitrogen transportation.
● I1F2 had the highest comprehensive evaluation index of maize.
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摘要  

确保为全球人口提供充足且有营养的粮食供应是农业生产实践中一项重大挑战。水肥是作物产量及品质提升的主要限制因素。然而,有机肥代替部分化学氮肥结合减灌能否通过改善光合生理特征和氮素转运来提高玉米产量和品质尚不清楚。2021‒2023年在西北干旱区开展玉米裂区田间试验。主区为两种灌水水平,当地传统灌溉(I2, 4,050 m3 ha‒1)和减量20%灌溉(I1, 3,240 m3 ha‒1)。裂区为5种有机无机肥等氮替代比例,分别为单施化学氮肥(F1)、有机肥替代化学氮肥25%(F2)、50%(F3)、75%(F4)、100%(F5)。探讨了减量灌溉条件下有机肥与化学氮肥配施对玉米产量、品质、光合生理特征及氮素转运的影响。结果表明,减量灌溉会降低玉米产量和品质。然而,有机肥替代25%化学氮肥可提高玉米产量和品质,且效果优于其他有机肥替代化学氮肥比例。与当地传统灌溉和单施化学氮肥(I2F1)相比,减灌20%与有机肥替代25%化学氮肥(I1F2)分别提高玉米籽粒产量和生物量13.0%和8.9%。与I2F1相比,I1F2提高玉米籽粒蛋白质含量10.0%,增加氨基酸和B族维生素含量分别为60.4和30.6%,提高秸秆粗脂肪和粗蛋白质含量分别为23.1和5.6%。I1F2提高玉米产量和品质的原因为(1)分别提高了R2‒R4期叶面积指数和光合势6.2和4.1%,净光合速率43.8%,丙酮酸磷酸二激酶、磷酸烯醇丙酮酸羧激酶和Rubisco酶活性9.8、9.7和10.5%;(2)分别增加R1期后氮素吸收和氮素积累5.6和5.4%,R1期前氮素转运量保持不变。因此,在干旱灌区,减量20%灌溉与有机肥替代25%化学氮肥能够通过改善玉米光合生理特征和氮素转运来提高玉米产量和品质。



Abstract  

Ensuring an adequate and nutritious food supply for the global population is a significant challenge in agricultural production practice.  Water and fertilizer are the main limiting factors in improving crop yield and quality.  However, it remains unclear whether the substitution of a portion of chemical nitrogen fertilizer with organic fertilizer, combined with reduced irrigation, can increase maize yield and quality through improvements in photo-physiological traits and nitrogen transportation.  A split-plot field experiment of maize was established in an arid area of northwestern China from 2021 to 2023.  Two irrigation levels formed the main plot: local conventional irrigation (I2, 4,050 m3 ha−1) and reduced by 20% (I1, 3,240 m3 ha−1).  Five equivalent nitrogen substitution ratios of chemical nitrogen with organic fertilizers formed a split plot, including sole chemical nitrogen fertilizer (F1), organic fertilizer substituting for 25% (F2), 50% (F3), 75% (F4), and 100% (F5) of chemical nitrogen fertilizer.  This study investigated the effects of combining organic and chemical nitrogen fertilizers under reduced irrigation on maize yield, quality, photo-physiological traits, and nitrogen transportation.  The results showed that reduced irrigation decreased maize yield and quality.  However, organic fertilizer substitution for 25% of chemical nitrogen increased maize yield and quality, with this effect greater than that observed with other equivalent nitrogen substitution ratios of chemical fertilizer with organic fertilizer.  A 20% reduction in irrigation combined with organic fertilizer substitution for 25% of chemical nitrogen (I1F2) increased maize grain yield and biomass by 13.0 and 8.9%, respectively, compared to local conventional irrigation and sole chemical nitrogen fertilizer (I2F1).  Meanwhile, I1F2 improved grain protein content by 10.0%, enhanced amino acid and vitamin B contents by 60.4 and 30.6%, and raised straw crude fat and crude protein contents by 23.1 and 5.6% compared to I2F1 in maize, respectively.  The reason for improving maize yield and quality with I1F2 was attributed to (1) improving leaf area index and leaf area duration at the blister (R2)−dough (R4) stage by 6.2 and 4.1%, increasing net photosynthetic rate by 43.8%, and enhancing pyruvate phosphate dikinase, phosphoenolpyruvate carboxykinase, and Rubisco activities by 9.8, 9.7, and 10.5%, respectively; (2) promoting nitrogen uptake and nitrogen accumulation after the R1 stage by 5.6 and 5.4% while maintaining nitrogen transportation quantity before the R1 stage.  Therefore, reducing irrigation by 20% combined with organic fertilizer substitution for 25% of chemical nitrogen can improve maize yield and quality via improving photo-physiological traits and nitrogen transportation in arid irrigation areas.

Keywords:  reduced irrigation       organic fertilizer substitution with partial chemical nitrogen        maize yield and quality        photo-physiological traits         nitrogen uptake and transportation  
Received: 07 February 2025   Accepted: 25 April 2025 Online: 27 May 2025  
Fund: 

We are very grateful for financial support of the National Key Research and Development Program of China (2023YFD1900405), the Science and Technology Program of Gansu Province, China (25JRRA347, 24ZDNA008, and 23JRRA704), the Industrial Support Project of Educational Committee of Gansu Province, China (2025CYZC-037), the Lanzhou Youth Science and Technology Talent Innovation Project of China (2023-QN-182), the Northwest Normal University Young Teachers Research Ability Enhancement Project of China (NWNU-LKQN2023-09), and the China Agricultural University Corresponding Support Research Joint Fund (GSAU–DKZY–2024–001).

About author:  #Correspondence Wen Yin, E-mail: yinwen@gsau.edu.cn

Cite this article: 

Feier Wang, Yao Guo, Pan Li, Xiayu Wu, Hailong Qiu, Wen Yin, Lianhao Zhao, Zhilong Fan, Falong Hu, Wei He, Hong Fan, Qiang Chai. 2026. Organic fertilizer substitution enhances maize yield and quality under reduced irrigation. Journal of Integrative Agriculture, 25(10): 4126-4141.

Abd-Elrahman S H, Saudy H S, Abd-El-Fattah D A, Hashem F A E. 2022. Effect of irrigation water and organic fertilizer on reducing nitrate accumulation and boosting lettuce productivity. Journal of Soil Science and Plant Nutrition, 22, 2144−2155.

Ahmad I, Yan Z G, Kamran M, Ikram K, Ghani M U, Hou F J. 2022. Nitrogen management and supplemental irrigation affected greenhouse gas emissions, yield and nutritional quality of fodder maize in an arid region. Agricultural Water Management, 269, 107650.

GB 5009.5-2016. 2016. Determination of Protein in Foods. National Health and Family Planning Commission of the People’s Republic of China, China Food and Drug Administration. China Standards Press, Beijing. (in Chinese)

GB/T 6438-2007. 2007. Determination of Crude Ash in Feeds. Standardization Administration of China. China Standards Press, Beijing. (in Chinese)

Chao Y, Fu G F, Yan X H, Hang Z Q, Yang Q G, Wang H, Pan H, Lou Y H, Zhu Y P. 2022. Effects of organic fertilizer on crop quality, soil fertility and environment: Research progress. Chinese Agricultural Science Bulletin, 38, 103−107. (in Chinese)

Chen N N, Ji R P, Jia Q Y, Feng R, Mi N, Zhang S J, Zhang Y S, Yu W Y. 2021. Effects of drought stress at key growth stages on yield and grain quality of spring maize. Chinese Journal of Ecology, 40, 1687−1694. (in Chinese)

Chen S C, Liu W F, Morel J L, Parsons D, Du T S. 2023. Improving yield, quality, and environmental co-benefits through optimized irrigation and nitrogen management of hybrid maize in Northwest China. Agricultural Water Management, 290, 108577.

Chen X Y, Ren H, Zhang J W, Zhao B, Ren B Z, Wan Y S, Liu P. 2024. Deep phosphorus fertilizer placement increases maize productivity by improving root−shoot coordination and photosynthetic performance. Soil and Tillage Research, 235, 105915.

Cui H X, Luo Y L, Li C H, Chang Y L, Jin M, Li Y, Wang Z L. 2023. Effects of nitrogen forms on nitrogen utilization, yield, and quality of two wheat varieties with different gluten characteristics. European Journal of Agronomy, 149, 126919.

Deng X, Liu Y, Xu X X, Liu D M, Zhu G R, Yan X, Wang Z M, Yan Y M. 2018. Comparative proteome analysis of wheat fag leaves and developing grains under water deficit. Frontiers in Plant Science, 9, 425.

Dong F, Li F, Jia Y Q, Yang F, Yan Q Y, Lu J X, Shen Y T. 2022. Effect of nitrogen topdressing rate on yield and quality of black-grained wheat varieties (strains). Journal of Nuclear Agricultural Sciences, 36, 435−444. (in Chinese)

Effah Z, Li L, Xie J, Liu C, Xu A, Karikari B, Anwar S, Zeng M. 2023. Regulation of nitrogen metabolism, photosynthetic activity, and yield attributes of spring wheat by nitrogen fertilizer in the semi-arid Loess Plateau region. Journal of Plant Growth Regulation, 42, 1120−1133.

Gao C H, El-Sawah A M, Ali D F, Hamoud Y A, Shaghaleh H, Sheteiwy M S. 2020. The integration of bio and organic fertilizers improve plant growth, grain yield, quality and metabolism of hybrid maize (Zea mays L.). Agronomy, 10, 319.

Gao H J, Zhu P, Peng C, Zhang X Z, Li Q, Zhang W J. 2015. Effects of partially replacement of inorganic N with organicmaterials on nitrogen efficiency of spring maize and soil inorganic nitrogen content under the same N input. Journal of Plant Nutrition and Fertilizers, 21, 318−325. (in Chinese)

Gao J. 2006. Experimental Supervision of Plant Physiology. Higher Education Press, Beijing. (in Chinese)

García A C, Ambrosio de Souza L G, Pereira M G, Castro R N, García-Mina J M, Zonta E, Lisboa F J G, Ricardo L L B. 2016. Structure-property-function relationship in humic substances to explain the biological activity in plants. Scientific Reports, 6, 20798.

Geng Y H, Cao G J, Wang L C, Wang S H. 2019. Effects of equal chemical fertilizer substitutions with organic manure on yield, dry matter, and nitrogen uptake of spring maize and soil nitrogen distribution. PLoS ONE, 14, e0219512.

Grahmann K, Verhulst N, Peña R J, Buerkert A, Vargas-Rojas L, Govaerts B. 2014. Durum wheat (Triticum durum L.) quality and yield as affected by tillage-straw management and nitrogen fertilization practice under furrow-irrigated conditions. Field Crops Research, 164, 166−177.

Guo J J, Fan J L, Xiang Y Z, Zhang F C, Yan S C, Zhang X Y, Zheng J, Li Y P, Tang Z J, Li Z J. 2022. Coupling effects of irrigation amount and nitrogen fertilizer type on grain yield, water productivity and nitrogen use efficiency of drip-irrigated maize. Agricultural Water Management, 261, 107389.

Gutiérrez R A. 2012. Systems biology for enhanced plant nitrogen nutrition. Science, 336, 1673−1675.

Hao J H, Tan J, Zhang Y, Gu X, Zhu G, Wang S, Li J. 2024. Sewage sludge-derived nutrients and biostimulants stimulate rice leaf photosynthesis and root metabolism to enhance carbohydrate, nitrogen and antioxidants accumulation. Chemosphere, 352, 141335.

He H, Peng M W, Lu W D, Hou Z N, Li J H. 2022. Commercial organic fertilizer substitution increases wheat yield by improving soil quality. Science of the Total Environment, 851, 158132.

Huang H. 2023. Regulating summer maize root distribution and yield by combined application of organic manure and chemical fertilizer. Journal of Agriculture, 13, 13−17. (in Chinese)

Huang W J, Ma T F, Liu X Y, Xu Y H, Gu J T, Gu Y F, Yuan J, Wen T, Xue C, Shen Q R. 2024. Degradation of complex carbon sources in organic fertilizers facilitates nitrogen fixation. Journal of Agricultural and Food Chemistry, 72, 12988−13000.

Huang X, Wang C Y, Hou J F, Du C Y, Liu S J, Kang J, Lu H F, Xie Y X, Guo T C, Ma D Y. 2020. Coordination of carbon and nitrogen accumulation and translocation of winter wheat plant to improve grain yield and processing quality. Scientific Reports, 10, 10340.

Ibrahim M, Khan A, Anjum M A, Ali W, Akbar H. 2020. Mulching techniques: An approach for offsetting soil moisture deficit and enhancing manure mineralization during maize cultivation. Soil and Tillage Research, 200, 104631.

Ishfaq M, Wang Y Q, Xu J L, Hassan M U, Yuan H, Liu L L, He B Y, Ejaz I, White P J, Cakmak I, Chen W S, Wu J C, van der Werf W, Li C J, Zhang F S, Li X X. 2023. Improvement of nutritional quality of food crops with fertilizer: A global meta-analysis. Agronomy for Sustainable Development, 43, 74.

Jahangirlou M R, Akbari G A, Alahdadi I, Soufizadeh S, Parsons D. 2021. Grain quality of maize cultivars as a function of plantingdates, irrigation and nitrogen stress: A case study from semiarid conditions of Iran. Agriculture, 11, 11.

Jiang M H, Dong C, Bian W P, Zhang W B, Wang Y. 2024. Effects of different fertilization practices on maize yield, soil nutrients, soil moisture, and water use efficiency in northern China based on a meta-analysis. Scientific Reports, 14, 6480.

Kan Z R, Liu W X, Liu W S, Lal R, Dang Y P, Zhao X, Zhang H L. 2022. Mechanisms of soil organic carbon stability and its response to no-till: A global synthesis and perspective. Global Change Biology, 28, 693−710.

Kaplan M, Karaman K, Kardes Y M, Kale H. 2019. Phytic acid content and starch properties of maize (Zea mays L.): Effects of irrigation process and nitrogen fertilizer. Food Chemistry, 283, 375−380.

Khapte P S, Kumar P, Burman U, Kumar P. 2019. Deficit irrigation in tomato: Agronomical and physio-biochemical implications. Scientia Horticulturae, 248, 256−264.

Kresović B, Tapanarova A, Tomić Z, Životić L, Vujović D, Sredojević Z, Gajić B. 2016. Grain yield and water use efficiency of maize as influenced by different irrigation regimes through sprinkler irrigation under temperate climate. Agricultural Water Management, 169, 34−43.

Li G H, Zhao B, Dong S T, Zhang J W, Liu P, Lu W P. 2020. Controlled-release urea combining with optimal irrigation improved grain yield, nitrogen uptake, and growth of maize. Agricultural Water Management, 227, 105834.

Li J, Yang Q L, Shi Z T, Zang Z N, Liu X G. 2021. Effects of deficit irrigation and organic fertilizer on yield, saponin and disease incidence in Panax notoginseng under shaded conditions. Agricultural Water Management, 256, 107056.

Li S T, Liu X Y, He P. 2017. Analyses on nutrient requirements in current agriculture production in China. Journal of Plant Nutrition and Fertilizers, 23, 1416−1432. (in Chinese)

Liang Y Z, Tong L P, Wu D L, Guo Q K, Xu F H, Zhao J K. 2017. Effects of organic manure combined with inorganic fertilizer on soil nitrate, maize yield and N use efficiency. Journal of Maize Sciences, 25, 111−116. (in Chinese)

Liu J H, He Q J, Wu Y X, Xiao X, Sun W W, Lin Y J, Yi R, Pan X B. 2023. The effect of sowing date on the nutritional quality of kernels of various maize varieties in Northeast China. Agronomy, 13, 2543.

Liu S R, Cui S, Ying F Y, Nasar J, Wang Y, Gao Q. 2021. Simultaneous improvement of protein concentration and amino acid balance in maize grain by coordination application of nitrogen and sulfur. Journal of Cereal Science, 99, 103189.

Liu W B, Ling N, Luo G W, Guo J J, Zhu C, Xu Q C, Liu M Q, Shen Q R, Guo S W. 2021. Active phoD-harboring bacteria are enriched by long-term organic fertilization. Soil Biology and Biochemistry, 152, 108071.

Liu X, Liu H. 2018. Plant Physiology Experiment. Higher Education Press, Beijing. (in Chinese)

Lyu X K, Liu Y, Li N, Ku L B, Hou Y T, Wen X X. 2022. Foliar applications of various nitrogen (N) forms to winter wheat affect grain protein accumulation and quality via N metabolism and remobilization. The Crop Journal, 10, 1165−1177.

Mao W T, Zhang X C, Ma M S, Liu Y L, Yin J D. 2023. Effects of partial substitution of chemical fertilizer by organic fertilizer on yield, and water and fertilizer use efficiency of winter wheat under film mulching in dryland. Journal of Triticeae Crop, 43, 1587−1598. (in Chinese)

Mathan J, Singh A, Ranjan A. 2021. Sucrose transport and metabolism control carbon partitioning between stem and grain in rice. Journal of Experimental Botany, 72, 4355−4372.

Mi W H, Sun Y, Gao Q, Liu M Y, Wu L H. 2019. Changes in humus carbon fractions in paddy soil given different organic amendments and mineral fertilizers. Soil and Tillage Research, 195, 104421.

Niu X G, Yang R Q, Li M, Duan B H, Diao T T, Ma F, Guo L P. 2020. Effects of interaction between elevated atmospheric CO2 concentration and nitrogen fertilizer on photosynthetic characteristic and yield of maize. Chinese Journal of Eco-Agriculture, 28, 255−264. (in Chinese)

Olszewski J, Makowska M, Pszczółkowska A, Okorski A, Bieniaszewski T. 2014. The effect of nitrogen fertilization on flag leaf and ear photosynthesis and grain yield of spring wheat. Plant, Soil and Environment, 60, 531−536.

Qi D L, Wu Q, Zhu J Q. 2020. Nitrogen and phosphorus losses from paddy fields and the yield of rice with different water and nitrogen management practices. Scientific Reports, 10, 9734.

Qian Y S, Ma Q, Ren Z, Zhu G L, Zhu X K, Zhou G S. 2023. Optimizing the growth of silage maize by adjusting planting density and nitrogen application rate based on farmers’ conventional planting habits. Agronomy, 13, 2785.

Quemada M, Gabriel J L. 2016. Approaches for increasing nitrogen and water use efficiency simultaneously. Global Food Security, 9, 29−35.

Ren F L, Sun N, Misselbrook Tom, Wu L H, Xu M G, Zhang F S, Wen Xu. 2022. Responses of crop productivity and reactive nitrogen losses to the application of animal manure to China’s main crops: A meta-analysis. Science of the Total Environment, 850, 158064.

Samarappuli D, Berti M T. 2018. Intercropping forage sorghum with maize is a promising alternative to maize silage for biogas production. Journal of Cleaner Production, 194, 515−524.

Saudy H S, Hamed M F, Abd El-Momen W R, Hussein H. 2020. Nitrogen use rationalization and boosting wheat productivity by applying packages ‎of humic, amino acids, and microorganisms. Communications in Soil Science and Plant Analysis, 51, 1036−1047.

Shen X L, Yang Z L, Xue J J, Yang Y. 2012. Comparison of kjeldahl and dumas combustion methods for determination of nitrogen content in animal manures. Transactions of the Chinese Society of Agricultural Engineering, 28, 205−209.

Sheteiwy M S, Dong Q, An J Y, Song W J, Guan Y J, He F, Huang Y T, Hu J. 2017. Regulation of ZnO nanoparticles-induced physiological and molecular changes by seed priming with humic acid in Oryza sativa seedlings. Plant Growth Regulation, 83, 27−41.

Van Soest P J, Robertson J B, Lewis B A. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74, 3583−3597.

Urban A, Rogowski P, Wasilewska-Dębowska W, Romanowska E. 2021. Understanding maize response to nitrogen limitation in different light conditions for the improvement of photosynthesis. Plants, 10, 1932.

Wang C G, Zhao M J, Pei W D, Wang G X, Zhang H J, Lei G L, Zhang R H. 2020. Effects of nitrogen application rate on grain yield and forage quality of dual-purpose maize. Journal of Maize Sciences, 28, 148−153. (in Chinese)

Wang F, Xie R Z, Ming B, Wang K, Hou P, Chen J L, Liu G Z, Zhang G Q, Xue J, Li S K. 2021. Dry matter accumulation after silking and kernel weight are the key factors for increasing maize yield and water use efficiency. Agricultural Water Management, 254, 106938.

Wang L, Zhou H, Fei C. 2024. Substituting partial chemical nitrogen fertilizers with organic fertilizers maintains grain yield and increases nitrogen use efficiency in maize. Frontiers in Plant Science, 15, 1442123.

Wang N, Feng K Y, Nan H Y, Cong A Q, Zhang T H. 2023a. Effects of combined application of organic manure and chemical fertilizer ratio on water and nitrogen use efficiency of cotton under water deficit. Scientia Agricultura Sinica, 56, 1531−1546. (in Chinese)

Wang N, Zhang T H, Cong A Q, Lian J. 2023b. Integrated application of fertilization and reduced irrigation improved maize (Zea mays L.) yield, crop water productivity and nitrogen use efficiency in a semi-arid region. Agricultural Water Management, 289, 108566.

Wang W, Li M Y, Zhou R, Mo F, Khan A, Batool A, Zhang W, Lu J S, Zhu Y, Wang B Z, Yang Y M, Wang J, Tao X P, Xiong Y C. 2023. Leaf senescence, nitrogen remobilization, and productivity of maize in two semiarid intercropping systems. European Journal of Agronomy, 150, 126943.

Wei J G, Chai Q, Yin W, Fan H, Guo Y, Hu F L, Fan Z L, Wang Q M. 2024. Grain yield and N uptake of maize in response to increased plant density under reduced water and nitrogen supply conditions. Journal of Integrative Agriculture, 23, 122−140.

Wu G, Yang S, Luan C S, Wu Q, Lin L L, Li X X, Che Z, Zhou D B, Dong Z R, Song H. 2024. Partial organic substitution for synthetic fertilizer improves soil fertility and crop yields while mitigating N2O emissions in wheat–maize rotation system. European Journal of Agronomy, 154, 127077.

Xu Y, Luo B L, Jia R, Xiao J, Wang X Q, Yang Y D, Xue S, Zeng Z H, Brown R W, Zang H D. 2024. Quantifying synergies and trade-offs in the food–energy–soil–environment nexus under organic fertilization. Journal of Environmental Management, 349, 119526.

Ye Z C, Li J, Wang J, Zhang C, Liu G B, Dong Q G. 2021. Diversity and co-occurrence network modularization of bacterial communities determine soil fertility and crop yields in arid fertigation agroecosystems. Biology and Fertility of Soils, 57, 809−824.

Yu N N, Ren B Z, Zhao B, Liu P, Zhang J W. 2019. Effects of nitrogen application rate on grain filling characteristics and nutritional quality of summer maize. The Journal of Applied Ecology, 30, 3771−3776. (in Chinese)

Yu Z T, Islam S, She M, Diepeveen D, Zhang Y J, Tang G X, Zhang J J, Juhasz A, Yang R C, Ma W J. 2018. Wheat grain protein accumulation and polymerization mechanisms driven by nitrogen fertilization. The Plant Journal, 96, 1160−1177.

Zhang J L, Gao F, Lin Y J, Wang Y Y, Yang C T, Zhang F, Li Y H, Li X D. 2013. Differences in seed kernel quality and related enzyme activities of different quality type peanut cultivars. The Journal of Applied Ecology, 24, 481−487. (in Chinese)

Zhang P P, Ma G, Wang C Y, Lu H F, Li S S, Xie Y X, Ma D Y, Zhu Y J, Guo T C. 2017. Effect of irrigation and nitrogen application on grain amino acid composition and protein quality in winter wheat. PLoS ONE, 12, e0178494.

Zhang X J, Wei T B, Fan Z L, Chai Q. 2020. Photosynthetic source dynamics and yield performance of high density maize with reduced amount of water and nitrogen in oasis irrigation region. Journal of Nuclear Agricultural Sciences, 34, 1302−1310. (in Chinese)

Zhang X Y, Fang Q C, Zhang T, Ma W, Velthof G L, Hou Y, Oenema O, Zhang F S. 2020. Benefits and trade-offs of replacing synthetic fertilizers by animal manures in crop production in China: A meta-analysis. Global Change Biology, 26, 888−900.

Zhong Y X, Xu D C, Hebelstrup K H, Yang D L, Cai J, Wang X, Zhou Q, Cao W X, Dai T B, Jiang D. 2018. Nitrogen topdressing timing modifies free amino acids profiles and storage protein gene expression in wheat grain. BMC Plant Biology, 18, 353.

Zhou Y, Li Y M, Fan M P, Wang Z L, Xu Z, Zhang D, Zhao J X. 2019. Effects of nitrogen in organic manure replacing chemical nitrogenous fertilizer on aggregates of red soil, maize yield and quality. Crops, 35, 125−132.

Zhu H J, Tang Z, Shi A L, Wen T, Wen C, Xue H L, Wang X H. 2022. Effects of irrigation patterns and combined application of organic fertilizer on rice yield, photosynthetic characteristics and nitrogen use efficiency. Soils, 54, 700−707.


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