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Journal of Integrative Agriculture  2025, Vol. 24 Issue (8): 3291-3296    DOI: 10.1016/j.jia.2024.12.006
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A novel Bocaparvovirus in goats: Genetic features, phylogenetic analysis, and epidemiological implications

Kegu Ji’e1*, Falong Yang1, 2*, Ai Ran3, Yang Su1, Taichun Gao1, Lanmuyi Gou1, Shenglin Li1, Zihan Xia1, Keha-mo Abi1, 2#

1 College of Animal & Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China

2 Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Chengdu 610041, China

3 Animal Husbandry Science Research Institute, Ganzi Tibetan Autonomous Prefecture, Kangding 626000, China 

 Highlights 
First identification of Caprine Bocaparvovirus in goats.
Caprine Bocaparvovirus may be associated with diarrhea in goats.
There is potential for cross-species transmission between goats and the wild animal Elaphodus cephalophus.
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摘要  
Bocaparvovirus(博卡病毒)是细小病毒科博卡病毒属的成员,可能会引起新生动物严重腹泻等胃肠道症状。目的:本研究目的在于鉴定一株新的博卡病毒—山羊博卡病毒,并对其进行全基因组和系统发育分析,了解流行病学意义。方法:利用PCR的方法从腹泻粪便样本中扩增山羊博卡病毒的全基因组。利用ORFfinder在线软件,对其全基因组序列进行病毒开放阅读框的预测,并使用BioAider和BioEdit软件对全基因组序列、NS1、NP和VP1基因进行同源性分析。RDP4.0 and SimPlot 3.5.1软件用于分析山羊博卡病毒与其他博卡病毒的重组情况。利用IQ-TREE(1.6.12)软件,以最大似然法构建基于全基因组、NS1以及VP1基因的系统发育树。基于全基因组序列建立检测山羊博卡病毒的特异性PCR方法,并使用SPSS软件以卡方检验法对腹泻和非腹泻粪便样本的阳性率进行统计分析。结果:在腹泻山羊的粪便样本中,通过PCR成功获得了一株全长为5351个核苷酸的完整基因组,暂命名为CapBOV-SWUN,具有标准的博卡病毒基因组结构。与在野生动物Elaphodus cephalophus中发现的新型博卡病毒ECBOV-tdf70毒株具有87.77%的相似性。根据国际病毒分类委员会(ICTV)的标准,该毒株的NS1与ECBOV-tdf70毒株的氨基酸一致性为91.2%,应共同归类为一个新物种。系统发育分析表明, CapBOV-SWUN与ECBOV-tdf70毒株聚为一个分支,遗传关系最近。山羊粪便样本的总体阳性率为32.2%(132/410)。四川、湖南和重庆的山羊粪便样本阳性率分别为30.0%(95/317)、40.0%(16/40)和39.6%(21/53),且腹泻山羊的感染率显著高于无症状山羊(P<0.05)。结论:本研究在山羊粪便样本中鉴定了一种新型博卡病毒,在中国三个省份中均被检测到,且腹泻粪便样本中的阳性率显著高于非腹泻粪便样本。创新性:本研究首次在山羊中鉴定了山羊博卡病毒。发现山羊博卡病毒可能与山羊的腹泻相关,且该毒株在山羊与野生动物Elaphodus cephalophus中具有跨物种传播的潜在可能性。证实山羊博卡病毒已在山羊中广泛流行,为该病毒的流行病学和遗传多样性提供了宝贵的见解。


Received: 13 August 2024   Online: 05 December 2024   Accepted: 22 October 2024
Fund: 
This study was supported by the Natural Science Foundation of Sichuan Province, China (2024NSFSC1272), the Innovation Team Development Funds for Sichuan Mutton Goat & Sheep, China (SCCXTD-2024-14), Scientific and Technological Innovation Team for Qinghai-Tibetan Plateau Research in Southwest Minzu University, China (2024CXTD08).
About author:  Kegu Ji’e, E-mail: jekgcom@gmail.com; Falong Yang, E-mail: falong.yang@swun.edu.cn; #Correspondence Keha-mo Abi, E-mail: abikehamo@swun.edu.cn * These authors contributed equally to this study.

Cite this article: 

Kegu Ji’e, Falong Yang, Ai Ran, Yang Su, Taichun Gao, Lanmuyi Gou, Shenglin Li, Zihan Xia, Keha-mo Abi. 2025. A novel Bocaparvovirus in goats: Genetic features, phylogenetic analysis, and epidemiological implications. Journal of Integrative Agriculture, 24(8): 3291-3296.

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