Identification of a novel multi-drug resistant plasmid co-harbouring extended-spectrum β-Lactamase resistance genes blaPER-4 and blaOXA-10 in Moellerella wisconsensis of sheep

doi:10.1016/j.jia.2024.03.040

Journal of Integrative Agriculture

2024, Vol. 23

Issue (9): 3238-3242 DOI: 10.1016/j.jia.2024.03.040

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Identification of a novel multi-drug resistant plasmid co-harbouring extended-spectrum β-Lactamase resistance genes bla_PER-4 and bla_OXA-10 in Moellerella wisconsensis of sheep

Xueliang Zhao^1,2*, Yongqiang Miao^2*, Hongmei Chen³, Honghu Shan², Juan Wang², Yang Wang¹, Jianzhong Shen^1#, Zengqi Yang^2#

¹National Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
² College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China
³ College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China

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摘要

威斯康星米勒菌（Moellerella wisconsensis，M. wisconsensis）是一种新发现的潜在人畜共患致病菌，偶尔从人和动物中分离出来。为了解该菌的耐药表型及耐基因特征，本研究从中国西北地区575份羊肛门拭子样本中分离出一株产ESBLs广泛耐药（Extensively drug-resistant, XDR）的威斯康星米勒菌，命名为NX2019MW，采用琼脂平板稀释法进行最低抑菌浓度（Minimal inhibitory concentration, MIC）测定，并且对该菌进行全基因组测序。结果显示，该菌株对磺胺异噁唑、大观霉素、氨苄西林、四环素、氟苯尼考、头孢唑肟、头孢吡肟、头孢噻呋、头孢曲松、头孢克肟、博来霉素和恩诺沙星耐药，仅对美罗培南敏感。基因组分析表明，该菌株含有一条染色体和三个质粒，其中新型质粒pNX2019-MW1大小为219302 bp，且携带bla_PER-4 和 bla_OXA-10耐药基因。进一步分析表明，ISCR1可以介导全基因组测序（WGS）分析表明，NX2019MW的染色体长度为3437288 bp，含有三个环状质粒。其中，pNX2019-MW1属于新型质粒，长度为219 302 bp（GC含量为47.55%），携带aadB、catB8、bla_OXA-10、acc(6´)-Ib、qacEdelta、bla_PER-4、sul1和 mph(E)等8个耐药基因。pNX2019-MW1（219 kb）是由质粒 p99101（CP028385.1）和pPROV013-PER（OK500122.1）同源重组而来形成新的质粒。bla_PER-4的核心基因结构仍保留在ISEcp1-blaPER-4-gst的保守序列中，通过反向PCR发现，ISCR1可形成环状中间体介导bla_PER-4水平传播。结合试验表明，携带ESBLs耐药基因的质粒pNX2019-MW1可在M. wisconsensis和受体菌株J53之间进行水平转移。因此，应加强对羊、人类和环境的中产ESBLs的M. wisconsensis监测，以防止耐药基因沿全链条进行传播。

Received: 30 October 2023 Accepted: 22 January 2024

Fund: The work was funded by the National Natural Science Foundation of China (32141002), the Postdoctoral Fellowship Program of CPSF under Grant, China (GZC20241939), and the China Agriculture Research System of MOF and MARA (CARS-39-14).

About author: Xueliang Zhao, E-mail: zhaoxueliang92@sina.com; Yongqiang Miao, E-mail: 2020065012@nwafu.edu.cn; #Correspondence Jianzhong Shen, Tel: +86-10-62732803, E-mail: sjz@cau.edu.cn; Zengqi Yang, Tel: +86-29-87092491, E-mail: yzq8126@163.com * These authors contributed equally to this study * These authors contributed equally to this study

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Cite this article:

Xueliang Zhao, Yongqiang Miao, Hongmei Chen, Honghu Shan, Juan Wang, Yang Wang, Jianzhong Shen, Zengqi Yang. 2024. Identification of a novel multi-drug resistant plasmid co-harbouring extended-spectrum β-Lactamase resistance genes bla_PER-4 and bla_OXA-10 in Moellerella wisconsensis of sheep. Journal of Integrative Agriculture, 23(9): 3238-3242.

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