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Journal of Integrative Agriculture  2024, Vol. 23 Issue (1): 348-353    DOI: 10.1016/j.jia.2023.09.026
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Emergence of highly pathogenic avian influenza A (H5N8) clade 2.3.4.4b viruses in grebes in Inner Mongolia and Ningxia, China, in 2021

Qiuzi Xu1*, Xinru Lü1*, Yi Li1*, Hua Luo1, Zhen Zhang1, Xiang Li1, Rongxiu Qin1, Qing An1, Fengyi Qu1, Zhenliang Zhao1, Chengbo Zhang4, Weidong Wang5, Yuecheng Li5, Yajun Wang1, Xiangwei Zeng1, Zhijun Hou1, Jingqiang Ren3#, Yulong Wang1#, Yanbing Li2#, Hongliang Chai1# 

1 College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China

2 Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150040, China

3 Wenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou 325000, China

4 Ordos Forestry and Grassland Administration, Ordos 017000, China

5 Monitoring Center for Terrestrial Wildlife Epidemic Diseases, Yinchuan 750000, China

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摘要  

20215月至6月,在中国内蒙古和宁夏地区死亡䴙䴘及其周围环境样监测到10H5N8亚型高致病性禽流感病毒,10H5N8亚型病毒的序列分析和遗传衍化结果显示,该病毒属于2.3.4.4b分支,碱性裂解位点为REKRRKR↓GLF,符合高致病性禽流感病毒分子特征。在遗传进化上,除分离株E42NP基因外,其余分离株的所有基因片段高度同源且2020年秋季在中国流行的H5N8亚型病毒具有相同进化来源;毒株E42NP基因则与2019-2020年在中亚水禽中流行的低致病性禽流感病毒聚类在一起。分析表明,自2020年秋季以来,H5N8亚型高致病性禽流感病毒在中国持续存在,并在进化过程中与水禽致病性禽流感病毒发生了重组

为了更加深入的了解H5N8亚型禽流感病毒的传播动态,我们选取了2020年秋季在中国流行的H5N8亚型高致病性禽流感病毒毒株与本研究H5N8亚型分离株的全基因组进行单倍型遗传网络分析,结果表明,本次H5N8亚型分离株与2020年秋季在湖北、河南三门峡和内蒙古发生的H5N8亚型病毒之间的核苷酸差异性较低,表明病毒可能在它们之间存在传播。

现场调查发现:20215月下旬以来,采样区域的黑颈䴙䴘种群开始发病死亡,并且出现扭颈、共济失调等禽流感典型神经症状结合已有研究数据,可以得出:䴙䴘对H5亚型高致病性禽流感病毒具有较高易感性,可以作为高致病性禽流感病毒的指示物种,进行长期监测和预警



Received: 25 May 2023   Accepted: 15 August 2023
Fund: 

This work was supported by the National Natural Science Foundation of China (31970501), the Fundamental Research Funds for the Central Universities, China (2572022CG01), and the National Forestry and Grassland Administration, China. 

About author:  #Correspondence Hongliang Chai, Tel: +86-18745051188, E-mail: Hongliang_Chai@hotmail.com; Yanbing Li, Tel: +86-451-51997168, E-mail: liyanbing@caas.cn; Yulong Wang, Tel: +86-13946025335, E-mail: yulongwang73@hotmail.com; Jingqiang Ren, Tel: +86-15943085036, E-mail: rjq207@163.com * These authors contributed equally to this study.

Cite this article: 

Qiuzi Xu, Xinru Lü, Yi Li, Hua Luo, Zhen Zhang, Xiang Li, Rongxiu Qin, Qing An, Fengyi Qu, Zhenliang Zhao, Chengbo Zhang, Weidong Wang, Yuecheng Li, Yajun Wang, Xiangwei Zeng, Zhijun Hou, Jingqiang Ren, Yulong Wang, Yanbing Li, Hongliang Chai. 2024.

Emergence of highly pathogenic avian influenza A (H5N8) clade 2.3.4.4b viruses in grebes in Inner Mongolia and Ningxia, China, in 2021 . Journal of Integrative Agriculture, 23(1): 348-353.

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