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Journal of Integrative Agriculture  2023, Vol. 22 Issue (11): 3560-3563    DOI: 10.1016/j.jia.2023.09.021
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Virucidal activity of MICRO-CHEM PLUS against African swine fever virus

JIANG Cheng-gang1, 2, SUN Ying2, ZHANG Fan2, AI Xin2, LU Ming2, Qin Jia-lin2, ZHANG Xian-feng1, 2, WANG Jing-fei1, BU Zhi-gao1, 2, ZHAO Dong-ming1#, HE Xi-jun1, 2#

1 State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.China
2 National High Containment Laboratory for Animal Diseases Control and Prevention, Harbin 150069, P.R.China
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摘要  

非洲猪瘟在我国爆发,给我国养殖业带来巨大的经济损失,目前无可用的商业化疫苗和药物,其相关研究限定于在高等级生物安全实验室中完成,生物安全防护中消毒剂的合理使用尤为重要。MICRO-CHEM PLUSMCP)作为一种复合型季铵盐类消毒剂在高等级生物安全实验室广泛使用,而针对非洲猪瘟病毒(African swine fever VirusASFV)的灭活效果未见相关报道。本文我们探究不同病毒载量、不同消毒剂浓度、不同作用时间及不同作用温度对于灭活非洲猪瘟病毒效果的影响。研究结果表明高病毒载量需要更高浓度的MCP才能将ASFV完全灭活,较低浓度的MCP需要延长作用时间才能达到完全灭活ASFV的效果,不同的作用温度对于MCP灭活ASFV的效果无影响。应用干雾消毒机将5%MCP进行房间喷雾消毒,当浓度达到0.06L/m3,用ASFV、大肠杆菌和金色葡萄球菌作为生物指示剂,可以达到终末消毒效果,但对于枯草芽孢杆菌作为生物指示剂还有部分活菌残留;当浓度为0.03L/m3时,对于大肠杆菌或金黄色葡萄球菌作为生物指示剂,也可以达到了终末消毒效果。该研究为在特定环境中合理使用 MCP 提供了科学依据,可以用于操作ASFV的高等级生物安全实验室的消毒以及猪场非洲猪瘟感染的预防。



Received: 04 July 2023   Accepted: 10 August 2023
Fund: This study was supported by the National Key Research and Development Program of China (2021YFC2600305 and 2021YFD1801405).

About author:  JIANG Cheng-gang, E-mail: jiangchenggang01@caas.cn; #Correspondence ZHAO Dong-ming, Tel: +86-451-51051815, E-mail: zhaodongming@caas.cn; HE Xi-jun, Tel: +86-451-51051781, E-mail: hexijun@caas.cn

Cite this article: 

JIANG Cheng-gang, SUN Ying, ZHANG Fan, AI Xin, LU Ming, QIN Jia-lin, ZHANG Xian-feng, WANG Jing-fei, BU Zhi-gao, ZHAO Dong-ming, HE Xi-jun. 2023. Virucidal activity of MICRO-CHEM PLUS against African swine fever virus. Journal of Integrative Agriculture, 22(11): 3560-3563.

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