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Journal of Integrative Agriculture  2022, Vol. 21 Issue (3): 898-899    DOI: 10.1016/S2095-3119(21)63845-5
Special Issue: 线虫合辑Nematology
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First record of the golden potato nematode Globodera rostochiensis in Yunnan and Sichuan provinces of China
JIANG Ru1, PENG Huan1, LI Yun-qing1, LIU Hui2, ZHAO Shou-qi2, LONG Hai-bo3, HU Xian-qi4, GE Jian-jun5, LI Xing-yue6, LIU Miao-yan6, SHAO Bao-lin7, PENG De-liang1
1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China.
2 The National Agro-Tech Extension and Service Center, Beijing 100026, P.R.China 
3 Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan,  P.R.China
4 State Key Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Agricultural University, Kunming 650201, P.R.China
5 Chinese Academy of Inspection and Quarantine, Beijing 100176, P.R.China
6 Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, P.R.China
7 Technical Center of Chengdu Customs, Chengdu 610095, P.R.China
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摘要  

马铃薯孢囊线虫Globodera rostochiensis是国际公认的重要检疫性有害线虫,严重危害马铃薯。2018-2020年,在全国农业技术推广服务中心组织的全国马铃薯检疫性线虫调查中,从云南省昭通市鲁甸县和四川省越西县和昭觉县马铃薯根系发现金色孢囊线虫,经形态学观察鉴定、分子生物学rDNA-ITS及28S的D2-D3区域特征分析比对及种特异性引物ITS5和PITSr3检测确定为马铃薯金线虫Globodera rostochiensis(Wollenweber)Skarbilovich, 1959. 在隔离温室内采用盆栽人工接种的致病性研究结果表明,该三个金线虫种群都能侵染马铃薯(品种青薯9号)并繁殖,接种12周后,马铃薯上形成成熟雌虫,完成生活史。这是马铃薯金线虫在我国云南和四川省首次记录。




Abstract  The potato cyst nematodes (PCN) Globodera rostochiensis (Wollenweber) Skarbilovich, 1959 is considered the most damaging nematode pest of potato worldwide that causes significant yield losses, and this nematode is recognized and listed as a quarantine nematode in many countries (EPPO 2017).  China is currently the largest producer of potato in the world, while the total production is also the highest (Guan and Cai 2019).  The survey for cyst nematodes on potato were conducted in Yunnan and Sichuan provinces of China during 2018–2020, numerous cysts were observed on potato roots in Huize County and Ludian County of Yunnan Province, Zhaojue County and Yuexi County of Sichuan Province.  Cysts and second-stage juveniles (J2s) were isolated from each soil sample using the Cobb decanting and sieving method.  The morphology of cysts and J2s and molecular analysis established the identity of this species as golden cyst nematode Globodera rostochiensis (Subbotin et al. 2010).  For morphological analysis, the cysts were characterized by smoothly rounded with a small projecting neck, brown and golden color, terminal cone was absent and circumfenestrate.  The key morphometrics of cysts (n=25) were: length excluding neck 705±24 (689–747) μm, width 698±28 (678–759) μm, number of cuticular ridges between anus and vulval fenestra 17.3±1.7 (14–19); fenestral diameter 13.6±1.1 (12.25–15.45) μm; distance from anus to the edge of fenestra 63.7±11.3 (48.23–79.14) μm; Granek’s ratio 4.7±0.7 (3.92–5.75).  The key morphometrics of J2s (n=25): body length 453.9±16.6 (440–496) μm, stylet length 21.9±1.0 (20.3–24.3) μm, tail length 51.1±3.2 (45.5–55.5) μm, and hyaline region length 24.4±2.5 (21.7–29.9) μm.  Morphology of the cysts and J2 were consistent with those of G. rostochiensis (Subbotin et al. 2010; EPPO 2017).  Moreover, the identification result was confirmed by PCR using universal primers TW81 (5´-GTTTCCGTAGGTGAACCTGC-3´) and AB28 (5´-ATATGCTTAAGTTCAGCGGGT-3´) for ITS region and D2A (5´-TTTTTTGGGCATCCTGAGGTTTAT-3´) D3B (5´-AGCACCTAAACTTAAAACATAATGAAAATG-3´) for rDNA-28S region, respectively.  The ITS rDNA sequences (GenBank accessions MZ042365, MZ042366, MZ042369, and MZ042370) exhibited 99.83% identity match to G. rostochiensis sequences available in the GenBank (GQ294513).  Sequence from the 28S region (GenBank accessions MZ057595, MZ057596, MZ057599, and MZ057600) was 99.33% similar to those of G. rostochiensis isolate from MF773722.  The species was also confirmed with species-specific primers ITS5 (5´-GGAAGTAAAAGTCGTAACAAGG-3´) and PITSr3 (5´-AGCGCAGACATGCCGCAA-3´) (Bulman and Marshall 1997), a single 434-bp fragment was obtained from Huize, Ludian, Zhaojue and Yuexi populations.  The pathog enicity testing of Huize, Ludian, Zhaojue and Yuexi, three weeks-old potato plants (cv. Qinshu 9)

were inoculated with 2 000 eggs, and cultured in an incubator at 23°C/20°C with a 16 h/8 h light/dark photoperiod.  After three months inoculation, 36±7.2 cysts and females were extracted from the infested potato roots, no females and cysts were observed on control plants.  


This is the first report of potato golden cyst nematode G. rostochiensis in China.  



Received: 28 June 2021   Accepted: 22 September 2021
Fund: This research was supported by the National Natural Science Foundation of China (32072398) and the Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences, China (ASTIP-02-IPP-04).
About author:  JIANG Ru, E-mail: jiangruby@126.com; Correspondence PENG De-liang, E-mail: pengdeliang@caas.cn

Cite this article: 

JIANG Ru, PENG Huan, LI Yun-qing, LIU Hui, ZHAO Shou-qi, LONG Hai-bo, HU Xian-qi, GE Jian-jun, LI Xing-yue, LIU Miao-yan, SHAO Bao-lin, PENG De-liang. 2022. First record of the golden potato nematode Globodera rostochiensis in Yunnan and Sichuan provinces of China. Journal of Integrative Agriculture, 21(3): 898-899.

Bulman S, Marshall J. 1997. Differentiation of Australasian potato cyst nematode (PCN) populations using the polymerase chain reaction (PCR). New Zealand Journal of Crop and Horticultural Science, 25, 123–129.
EPPO. 2017. PM 7/40 (4) Globodera rostochiensis and Globodera pallida. EPPO Bulletin, 47, 174–197.
Guan J C, Cai H L. 2019. Analysis on characteristics and causes of potato production pattern in China. Journal of China Agricultural Resources and Regional Planning, 40, 92–100. 
Subbotin S A, MundoOcampo M, Baldwin J G, Subbotin S A, MundoOcampo M, Baldwin J G. 2010. Systematics of Cyst Nematodes (Nematoda: Heteroderinae). vol. 8. Part A. Hunt D J, Perry R N, eds., Leiden, Brill, the Netherlands. pp. 113–123.
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