|Field mold stress induced catabolism of storage reserves in soybean seed and the resulting deterioration of seed quality in the field
|DENG Jun-cai1, 2, LI Xiao-man1, 2, 3, XIAO Xin-li1, 2, WU Hai-jun1, 2, YANG Cai-qiong1, 2, 3, LONG Xi-yang1, 2, ZHANG Qi-hui1, 2, Nasir Iqbal1, 2, 5, WANG Xiao-chun1, 2, YONG Tai-wen1, 2, DU Jun-bo1, 2, 3, YANG Feng1, 2, LIU Wei-guo1, 2, 3, ZHANG Jing1, 4, WU Xiao-ling1, 2, WU Yu-shan1, 2, YANG Wen-yu1, 2, LIU Jiang1, 2, 3
|1 Sichuan Engineering Research Center for Crop Strip Intercropping System/Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture and Rural Affairs, Chengdu 611130, P.R.China
2 College of Agriculture, Sichuan Agricultural University, Chengdu 611130, P.R.China
3 Institute of Ecological Agriculture, Sichuan Agricultural University, Chengdu 611130, P.R.China
4 College of Horticulture, Sichuan Agricultural University, Chengdu 611130, P.R.China
5 School of Agriculture, Food and Wine, The University of Adelaide, Urrbrae, SA 5064, Australia
Abstract Excessive rainfall provides a favorable condition for field mold infection of plants, which triggers field mold (FM) stress. If FM stress occurs during the late maturation stage of soybean seed, it negatively affects seed yield and quality. To investigate the responses of soybean seed against FM stress and identify the underlying biochemical pathways involved, a greenhouse was equipped with an artificial rain producing system to allow the induction of mold growth on soybean seed. The induced quality changes and stress responses were revealed on the levels of both transcriptome and metabolome. The results showed that soybean seeds produced under FM stress conditions had an abnormal and inferior appearance, and also contained less storage reserves, such as protein and polysaccharide. Transcriptional analysis demonstrated that genes involved in amino acid metabolism, glycolysis, tricarboxylic acid, β-oxidation of fatty acids, and isoflavone biosynthesis were induced by FM stress. These results were supported by a multiple metabolic analysis which exhibited increases in the concentrations of a variety of amino acids, sugars, organic acids, and isoflavones, as well as reductions of several fatty acids. Reprogramming of these metabolic pathways mobilized and consumed stored protein, sugar and fatty acid reserves in the soybean seed in order to meet the energy and substrate demand on the defense system, but led to deterioration of seed quality. In general, FM stress induced catabolism of storage reserves and diminished the quality of soybean seed in the field. This study provides a more profound insight into seed deterioration caused by FM stress.
Received: 14 August 2020
Accepted: 17 December 2020
|Fund: This work was supported by the National Natural Science Foundation of China (31971853), the National Key Research and Development Program of China (2016YFD0300209) and the Science Fund for Distinguished Young Scholars of Sichuan, China (20JCQN0155).
|About author: Correspondence YANG Wen-yu, E-mail: firstname.lastname@example.org; LIU Jiang, E-mail: email@example.com
Cite this article:
DENG Jun-cai, LI Xiao-man, XIAO Xin-li, WU Hai-jun, YANG Cai-qiong, LONG Xi-yang, ZHANG Qi-hui, Nasir Iqbal, WANG Xiao-chun, YONG Tai-wen, DU Jun-bo, YANG Feng, LIU Wei-guo, ZHANG Jing, WU Xiao-ling, WU Yu-shan, YANG Wen-yu, LIU Jiang.
Field mold stress induced catabolism of storage reserves in soybean seed and the resulting deterioration of seed quality in the field. Journal of Integrative Agriculture, 21(2): 336-350.
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