Special Issue:
植物病理合辑Plant Protection—Plant Pathology
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The putative elongator complex protein Elp3 is involved in asexual development and pathogenicity by regulating autophagy in the rice blast fungus |
ZHANG Li-mei1*, CHEN Shu-ting1*, QI Min1, 2*, CAO Xue-qi1, LIANG Nan1, LI Qian3, TANG Wei1, LU Guo-dong1, ZHOU Jie1, 2, YU Wen-ying1, 2, WANG Zong-hua1, 4, ZHENG Hua-kun1, 5 |
1 State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China
2 Fujian Universities Key Laboratory for Plant–Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China
3 Meishan Vocational and Technical College, Meishan 620010, P.R.China
4 Institute of Oceanography, Minjiang University, Fuzhou 350108, P.R.China
5 National Engineering Research Center of JUNCAO Technology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China |
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摘要
细胞自噬通过维持细胞内物质与能量的动态平衡,进而调控很多发育过程。已知延伸复合物蛋白Elp3具有多种功能并参与调控自噬,但其在稻瘟病菌中的功能仍不清楚。为此,构建了稻瘟病菌Elp3编码基因PoELP3(MGG_05481)的敲除突变体,对其功能进行研究。表型分析结果显示,PoELP3基因的敲除导致稻瘟病菌菌丝生长受抑制,产孢量下降,对细胞壁胁迫剂和盐胁迫剂的敏感性增强,附着胞膨压和致病性显著下降。这些结果表明稻瘟病菌PoElp3在生长发育、胁迫响应和致病过程中均具有重要作用。亚细胞定位结果表明GFP-PoElp3融合蛋白定位于细胞核和细胞质中。为检测稻瘟病菌Elp3在细胞自噬中的作用,将自噬标记GFP-PoAtg8分别导入野生型和突变体中,并通过计算总蛋白中游离GFP占GFP-PoAtg8和游离GFP总量的比例,评估野生型和突变体中自噬的水平。结果表明,无论是在营养充分还是营养不足的条件下,△Poelp3突变体均呈现较高的自噬水平。这可能是导致菌丝生长缓慢的原因。此外,△Poelp3突变体营养菌丝和侵染菌丝的生长均对雷帕霉素更加敏感,但是PoELP3基因的缺失并不影响雷帕霉素对TOR-信号途径下游基因的转录抑制,暗示其并不参与TOR-信号的传导。综上所述,稻瘟病菌Elp3可以通过调控自噬影响无性发育和致病性。但是PoElp3调控自噬的机制仍有待进一步研究。
Abstract
Autophagy is responsible for maintaining fundamental cellular homeostasis and is, therefore, essential for diverse development processes. This study reported that PoElp3, the putative catalytic subunit of Elongator complex, is involved in the maintenance of autophagy homeostasis to facilitate asexual development and pathogenicity in the rice blast fungus Pyricularia oryzae. It was found that the ΔPoelp3 strains were defective in vegetative growth, conidiation, stress response, and pathogenicity. The mutants exhibited hyper-activated autophagy in the vegetative hyphae under both nutrient-rich and nutrient-deficient conditions. The hyper-activation of autophagy possibly suppressed the production of vegetative hyphae in the ΔPoelp3 strains. Moreover, the ΔPoelp3 strains were found to be more sensitive to rapamycin during vegetative- and invasive-hyphal growth but have no effect on Target-of-Rapamycin (TOR) signaling inhibition. Taken together, these results demonstrated that PoElp3 is involved in asexual development and pathogenicity by regulating autophagy in the rice blast fungus.
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Received: 08 July 2020
Accepted:
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Fund: This work was supported by grants from the National Natural Science Foundation of China (31770156 and 2016YFD0300700) and the Innovative Research Funding of FAFU, China (CXZX2018051 and CXZX2018052). |
Corresponding Authors:
Correspondence ZHENG Hua-kun, E-mail: huakunzheng@163.com; WANG Zong-hua, E-mail: zonghuaw@163.com; YU Wen-ying, E-mail: wenyingyu2004@126.com
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About author:
* These authors contributed equally to this study. |
Cite this article:
ZHANG Li-mei, CHEN Shu-ting, QI Min, CAO Xue-qi, LIANG Nan, LI Qian, TANG Wei, LU Guo-dong, ZHOU Jie, YU Wen-ying, WANG Zong-hua, ZHENG Hua-kun.
2021.
The putative elongator complex protein Elp3 is involved in asexual development and pathogenicity by regulating autophagy in the rice blast fungus. Journal of Integrative Agriculture, 20(11): 2944-2956.
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