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Journal of Integrative Agriculture  2022, Vol. 21 Issue (1): 178-187    DOI: 10.1016/S2095-3119(20)63445-1
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Population genetic structure of Sitobion miscanthi in China
SUN Jing-xuan1, 2, 3, LI Qian1, TAN Xiao-ling1, FAN Jia1, ZHANG Yong1, QIN Yao-guo1, Frédéric FRANCIS2, CHEN Ju-lian 
1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2 Department of Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liège, Gembloux 5030, Belgium
3 Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences, Mudanjiang 157000, P.R.China 
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Abstract  The wheat aphid, Sitobion miscanthi, is one of the most destructive pests of wheat plants in the temperate regions of China.  Little is known about the genetic structure evolution of  the different geographic populations of S. miscanthi with its migration.  In this study, we investigated the population genetic structure and demographic history of S. miscanthi by analysing 18 geographical populations across China using one mitochondrial gene, COI; one nuclear gene, EF-1α; and two endosymbiont Buchnera genes, gnd and trpA.  Analysis of data from the various groups showed high haplotype diversity and low nucleotide variation.  SAMOVA analysis did not find a correlation between genetic distance and geographic distance.  However, areas with high population diversity exhibited high haplotype diversity.  Therefore, we speculate that there are two main natural migration pathways of S. miscanthi in China.  One is from Yunnan to the Sichuan Basin, and the other is from Wuhan, Xinyang and Jiaodong Peninsula areas to the northwest.  Based on this hypothesis, we inferred that these aphid populations appear first in the southwestern and southern regions and spread to the north with the help of the southeastern and southwestern monsoons, which occur in spring and summer.  In autumn, the aphids spread southward with the northeastern and northwestern monsoons.

Keywords:  Sitobion miscanthi        molecular marker       COI        symbiotic bacteria        phylogenetic tree  
Received: 12 June 2020   Accepted: 21 September 2020
Fund: We would like to thank our technician Ms. Liu Yanxia for aphid rearing in our laboratory.  This study was funded by the National Natural Science Foundation of China (31871979, 32001900 and 31901881), the National Key R&D Program in China (2017YFD0201700, 2017YFD0200900 and 2016YFD0300700), and the China Agriculture Research System of MOF and MARA (CARS-22).

Cite this article: 

SUN Jing-xuan, LI Qian, TAN Xiao-ling, FAN Jia, ZHANG Yong, QIN Yao-guo, Frédéric FRANCIS, CHEN Ju-lian. 2022. Population genetic structure of Sitobion miscanthi in China. Journal of Integrative Agriculture, 21(1): 178-187.

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