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Journal of Integrative Agriculture  2020, Vol. 19 Issue (6): 1492-1500    DOI: 10.1016/S2095-3119(20)63167-7
Special Focus: Physiology and interaction of insects with environmental factors Advanced Online Publication | Current Issue | Archive | Adv Search |
Expression, regulation and binding affinity of fatty acid-binding protein 2 in Spodoptera litura
WEN Liang*, GAO Gui-ping*, HUANG Zhi-qiang, ZHENG Si-chun, FENG Qi-li, LIU Lin
Institute of Insect Science and Technology, School of Life Sciences, South China Normal University/Guangzhou Key Laboratory of Insect Development Regulation and Applied Research/Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou 510631, P.R.China
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Fatty acid-binding proteins (FABPs) are a family of lipid chaperones, which contribute to systemic metabolic regulation through diverse lipid signalings.  In this study, a midgut-specific FABP gene (Slfabp2) was cloned from Spodoptera litura.  RT-PCR and Western blot analysis indicated that RNA and protein levels of SlFABP2 gradually increased and reached a peak at the prepupal stage and maintained a high level during the pupal stage.  The expression of SlFABP2 protein was induced by starvation treatment.  In vitro binding assay revealed that the recombinant SlFABP2 had high affinities of binding long-chain fatty acids, such as palmitic acid, arachidonate and oleic acid.  The results suggest that SlFABP2 may have a unique function that transports intracellular fatty acids and can regulate the metabolism of lipids in metamorphosis.  This work provides experimental clues for understanding the potential function of SlFABP2 in fatty acid metabolism in S. litura.
Keywords:  Spodoptera litura        SlFABP2        starvation treatment        binding assay  
Received: 15 October 2019   Accepted: 26 April 2020
Fund: The study was supported by the National Natural Science Foundation of China (31330071 and 31772519) and the Natural Science Foundation of Guangdong Province, China (2017A030313210).
Corresponding Authors:  Correspondence LIU Lin, E-mail:    
About author:  * These authors contributed equally to this study.

Cite this article: 

WEN Liang, GAO Gui-ping, HUANG Zhi-qiang, ZHENG Si-chun, FENG Qi-li, LIU Lin. 2020. Expression, regulation and binding affinity of fatty acid-binding protein 2 in Spodoptera litura. Journal of Integrative Agriculture, 19(6): 1492-1500.

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