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Journal of Integrative Agriculture  2019, Vol. 18 Issue (5): 1072-1079    DOI: 10.1016/S2095-3119(19)62582-7
Animal Science · Veterinary Medicine Advanced Online Publication | Current Issue | Archive | Adv Search |
Production of homeobox A10 gene transgenic pigs by somatic cell nuclear transfer
XIAO Qian1, 2*, ZHAO Chang-zhi1*, LIN Rui-yi1, LI Guang-lei1, LI Chang-chun1, WANG Hai-yan1, XU Jing1, XIE Sheng-song1, 2, YU Mei1, ZHAO Shu-hong1  
1 Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R.China
2 College of Animal Science and Technology, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, P.R.China
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Homeobox A10 (Hoxa10) gene is one of the most important candidate genes associated with the reproductive performance of humans and mice.  Overexpression of Hoxa10 in mouse endometrium can increase litter size.  Moreover, Hoxa10 plays a key role in regulating the embryo implantation of sows.  This study aimed to generate transgenic pigs using Hoxa10 via somatic cell nuclear transfer (SCNT).  We established seven Hoxa10-transgenic cell lines, and two of the cell lines were selected as nuclear donors for the transfer.  A total of 1 270 cloned embryos were generated and transferred to five surrogate mothers (Landrace×Yorkshire).  Eight cloned male piglets were produced including one with cryptorchidism.  Six transgenic piglets grew up healthy and produced 56 offspring.  Finally, we obtained six transgenic male pigs and 26 transgenic positive offspring that can be used to further study the regulatory mechanism of Hoxa10 on the reproductive performance of pigs.
Keywords:  Hoxa10 gene        transgenic pig        somatic cell nuclear transfer        fetal fibroblasts        oocyte  
Received: 06 March 2018   Accepted:
Fund: This study was supported by the National Major Special Project on New Varieties Cultivation for Transgenic Organisms, China (2014ZX08006-005 and 2014ZX0800950B).
Corresponding Authors:  Correspondence ZHAO Shu-hong, E-mail:    
About author:  XIAO Qian, E-mail:; * These authors contributed equally to this study.

Cite this article: 

XIAO Qian, ZHAO Chang-zhi, LIN Rui-yi, LI Guang-lei, LI Chang-chu, WANG Hai-yan, XU Jing, XIE Sheng-song, YU Mei, ZHAO Shu-hong. 2019. Production of homeobox A10 gene transgenic pigs by somatic cell nuclear transfer. Journal of Integrative Agriculture, 18(5): 1072-1079.

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