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| Functional characterization of (E)-β-caryophyllene synthase from lima bean and its up-regulation by spider mites and alamethicin |
| LI Feng-qi1, 2, FU Ning-ning1, ZHOU Jing-jiang3, WANG Gui-rong2 |
1 Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, P.R.China
2 Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
3 Department of Biological Chemistry and Crop Protect, Rothamsted Research, Harpenden AL5 2JQ, UK |
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Abstract (E)-β-Caryophyllene is a sesquiterpene compound widely distributed in plants and functions in plant defence. However, little is known about the sequence and function of (E)-β-caryophyllene synthase in lima bean (Phaseolus lunatus). Here, we report a new full-length cDNA (PlCAHS) encoding (E)-β-caryophyllene synthase, a possible key enzyme of plant defence. The cDNA of PlCAHS contains an open reading frame of 1 761 bp, encoding a protein of 586 amino acids with a predicted mass of 67.95 kDa. The deduced amino acid sequence shows 52% identity with sesquiterpene synthase MtCAHS of Medicago truncatula. Based on phylogenetic analysis, PlCAHS is classified as the terpene synthases (TPS)-a subfamily. The recombinant enzyme, expressed in Escherichia coli, catalysed the formation of a major product (E)-β-caryophyllene (82%) and a minor product α-humulene (18%) from farnesyl diphosphate. Real-time quantitative PCR (qRT-PCR) analysis found that the PlCAHS transcript was significantly up-regulated in leaves after treatment with spider mites and alamethicin (ALA), suggesting its ecological function in plant defence.
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Received: 13 December 2016
Accepted:
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| Fund: This work was funded by the International Science and Technology Cooperation Program of China (2013DFG32230) and the Major Project of Genetically Modified Organisms Breeding, China (2016ZX08010005). |
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Corresponding Authors:
Correspondence WANG Gui-rong, E-mail: grwang@ippcaas.cn
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| About author: LI Feng-qi, E-mail: pandit@163.com |
Cite this article:
LI Feng-qi, FU Ning-ning, ZHOU Jing-jiang, WANG Gui-rong.
2017.
Functional characterization of (E)-β-caryophyllene synthase from lima bean and its up-regulation by spider mites and alamethicin. Journal of Integrative Agriculture, 16(10): 2231-2238.
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