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Journal of Integrative Agriculture  2017, Vol. 16 Issue (08): 1874-1878    DOI: 10.1016/S2095-3119(16)61531-9
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Penicillin-resistant characterization of Staphylococcus aureus isolated from bovine mastitis in Gansu, China
YANG Feng*, LIU Long-hai*, WANG Ling, WANG Xu-rong, LI Xin-pu, LUO Jin-yin, ZHANG Zhe, ZHANG Shi-dong, YAN Zuo-ting, LI Hong-sheng
Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, P.R.China
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Abstract      Bovine mastitis caused by Staphylococcus aureus is difficult to treat because of increasing resistance against antibiotics, especially penicillin. β-Lactamase and biofilm are responsible for penicillin resistance of S. aureus. The aim of this study was to investigate the β-lactamase activity and biofilm formation capacity of 37 penicillin-resistant S. aureus strains (35 were blaZ positive and 2 were blaZ negative) from bovine mastitis in Gansu Province, China, as well as to measure the intercellular adhesion genes icaA and icaD of these strains. β-Lactamase Test Kit was used to determine the β-lactamase activity, biofilm formation was tested by semi-quantitative adherence assay method. Moreover, the presence of icaA and icaD were measured by PCR. A total of 32 penicillin-resistant S. aureus strains, including the two blaZ-negative strains, were identified as β-lactamase producers. All tested S. aureus isolates produced biofilm in the microtiter plate assay. Meanwhile, all these strains were PCR-positive for the ica locus, icaA and icaD. The study indicated high prevalence of β-lactamase activity, biofilm-forming capacity, and the ica genes among the penicillin-resistant S. aureus isolates, and implied that S. aureus resistant to penicillin was attributed to multiple mechanisms.
Keywords:  Staphylococcus aureus        penicillin-resistant        β-lactamase        biofilm        ica  
Received: 21 June 2016   Accepted: 02 August 2017

This study was supported by the Central Public-Interest Scientific Institution Basal Research Fund, China (1610322015007), the Key Technologies R&D Program of China during the 12th Five-year Plan period (2012BAD12B03) and the Natural Science Foundation of Gansu Province, China (145RJYA311).

Corresponding Authors:  Correspondence YANG Feng, Tel: +86-931-2115262, Fax: +86-931-2114180, E-mail:; LI Hong-sheng, Tel: +86-931-2164183, Fax: +86-931-2114180, E-mail:   

Cite this article: 

YANG Feng, LIU Long-hai, WANG Ling, WANG Xu-rong, LI Xin-pu, LUO Jin-yin, ZHANG Zhe, ZHANG Shi-dong, YAN Zuo-ting, LI Hong-sheng. 2017. Penicillin-resistant characterization of Staphylococcus aureus isolated from bovine mastitis in Gansu, China. Journal of Integrative Agriculture, 16(08): 1874-1878.

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