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| Fluorescent competitive assay for melamine using dummy molecularly imprinted polymers as antibody mimics |
| DU Xin-wei1, 2, ZHANG Yan-xin3, SHE Yong-xin1, 2, LIU Guang-yang3, ZHAO Feng-nian1, 2, WANG Jing1, 2, WANG Shan-shan1, 2, JIN Fen1, 2, SHAO Hua1, 2, JIN Mao-jun1, 2, ZHENG Lu-fei1, 2 |
1 Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 Key Laboratory of Agro-product Quality and Safety, Beijing 100081, P.R.China
3 School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin 150001, P.R.China |
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Abstract A fluorescent competitive assay for melamine was first developed utilizing dummy molecularly imprinted polymers (DMIPs) as artificial antibodies. This method is based on the competition between fluorescent substances and the unlabeled analyte for binding sites in synthesized DMIPs and the decreased binding of fluorescent substances to DMIPs due to increased concentrations of melamine in the solutions. DMIPs for melamine were synthesized under a hot water bath in the presence of the initiator azobisisobutyronitrile (AIBN) using 2,4-diamino-6-methyl-1,3,5-triazine (DAMT) as a dummy template, methacrylic acid (MAA) as a functional monomer, and ethylene glycol dimethacrylate (EGDMA) as a crosslinking agent. The adsorption capacity and selectivity of DMIPs for melamine were evaluated by the isothermal adsorption curve and Scatchard analysis. The evaluation results showed that the synthesized DMIPs had specific recognition sites for melamine and the maximum adsorption amount was 1 066.33 μg g–1. Later, 5-(4,6-dichlorotriazinyl) amino fluorescein (DTAF) with a triazine ring, which slightly resembles melamine, was selected as the fluorescent substance. The fluorescent competitive assay using DMIPs as the antibody mimics was finally established by selecting and optimizing the reaction solvents, DMIPs amount, DTAF concentration, and incubation time. The optimal detection system showed a linear response within range of 0.05–40 mg L–1 and the limit of detection (LOD) was 1.23 μg L–1. It was successfully applied to the detection of melamine in spiked milk samples with satisfactory recoveries (71.9 to 86.3%). According to the comparative analysis, the result of optimized fluorescent competitive assay revealed excellent agreement with the HPLC-MS/MS result for melamine.
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Received: 13 May 2015
Accepted:
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| Fund: This work was supported by the National Natural Science Foundation of China (31260620, 31471654) and the Special Fund for Agro-scientific Research in the Public Interest of China (201203094) |
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Corresponding Authors:
WANG Jing, Tel: +86-10-82106568, E-mail: w_jing2001@126.com
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| About author: DU Xin-wei, E-mail: wduxinwei@126.com |
Cite this article:
DU Xin-wei, ZHANG Yan-xin, SHE Yong-xin, LIU Guang-yang, ZHAO Feng-nian, WANG Jing, WANG Shan-shan, JIN Fen, SHAO Hua, JIN Mao-jun, ZHENG Lu-fei.
2016.
Fluorescent competitive assay for melamine using dummy molecularly imprinted polymers as antibody mimics. Journal of Integrative Agriculture, 15(05): 1166-1177.
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