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| Characterization and function of Tomato yellow leaf curl virus-derived small RNAs generated in tolerant and susceptible tomato varieties |
| BAI Miao1*, YANG Guo-shun1*, CHEN Wen-ting1, LIN Run-mao2, LING Jian2, MAO Zhen-chuan2, XIE Bing-yan2 |
1 College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, P.R.China
2 Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China |
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Abstract Virus-tolerant plant, which allows the accumulation of virus and then generates virus-derived small RNAs (vsRNAs), is a valuable material to reveal the antiviral efficiency of vsRNAs. Here, a comparison of vsRNAs in Tomato yellow leaf curl virus tolerant and in susceptible tomato varieties showed the consistent trend of vsRNAs’ distribution on virus genome, which is presented as an obvious characteristic. However, the expression level of vsRNA in tolerant variety is less than that in susceptible variety. Slicing targets of vsRNA-mediated viral transcripts were investigated using parallel analysis of RNA ends, and geminivirus DNA methylation was determined by bisulfite sequencing, which uncovered that not all vsRNAs participated in viral mRNA degradation and DNA methylation. Additionally, by comparing with the expression pattern of vsRNAs, viral DNA and mRNA, we proposed the quantity of vsRNAs is corresponding to the expression level of viral mRNA, while the virus-suppression of vsRNAs is not high-efficient.
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Received: 29 October 2015
Accepted:
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| Fund: This work was supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China (20134320120013) and the Natural Science Foundation of Hunan Province, China (14JJ3095). |
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Corresponding Authors:
XIE Bing-yan, Tel/Fax: +86-10-82105979, E-mail: xiebingyan@caas.cn
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| About author: BAI Miao, Mobile: +86-18374879885, E-mail: baimiao1984@qq.com; |
Cite this article:
BAI Miao, YANG Guo-shun, CHEN Wen-ting, LIN Run-mao, LING Jian, MAO Zhen-chuan, XIE Bing-yan.
2016.
Characterization and function of Tomato yellow leaf curl virus-derived small RNAs generated in tolerant and susceptible tomato varieties. Journal of Integrative Agriculture, 15(8): 1785-1797.
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