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Journal of Integrative Agriculture  2016, Vol. 15 Issue (8): 1727-1743    DOI: 10.1016/S2095-3119(15)61288-6
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Overexpression of GmProT1 and GmProT2 increases tolerance to drought and salt stresses in transgenic Arabidopsis
GUO Na1*, XUE Dong1*, ZHANG Wei1, ZHAO Jin-ming1, XUE Chen-chen1, YAN Qiang1, XUE Jin-yan1, WANG Hai-tang1, ZHANG Yu-mei2, XING Han1
1 National Center for Soybean Improvement/Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture/State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, P.R.China
2 Institute of Crop Sciences, Fujian Academy of Agricultural Sciences, Fuzhou 350013, P.R.China
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Abstract     The proline transporter protein (ProT) plays an important role in protective stress responses in various plants. However, its function in abiotic stress responses in soybean (Glycine max) remains obscure. In the present study, two soybean ProT genes, namely GmProT1 and GmProT2, were isolated by homologous cloning. GmProT1 and GmProT2 encode polypeptides of 435 and 433 amino acids, respectively. The GmProT1 and GmProT2 proteins showed high similarity to other ProT proteins. GmProT1 and GmProT2 transcripts were detected in different soybean tissues including roots, stems, leaves, flowers, and developmental seeds, and during diverse developmental stages. GmProT1 was strongly expressed in seeds 35 days after flowering. Quantitative real-time PCR analysis showed that the two genes were highly expressed in leaves and could be strongly induced in response to salt and drought conditions and ABA treatment. Transgenic Arabidopsis thaliana plants overexpressing the two genes were generated, which showed that GmProT genes attenuate damage from salt and drought stress. In addition, transgenic Arabidopsis plants accumulated proline in response to salt and osmotic stress. Transcription levels of salinity-responsive gene (RD29B and S0S3) and drought-induced gene (CDPK1) were higher in the transgenic lines than that of wild type plants. Our work provides evidence that GmProT genes function in the response to abiotic stresses and may affect the synthesis and response system of proline.
Keywords:  Glycine max        salt and drought stresses        GmProT1        GmProT2   
Received: 31 August 2015   Accepted:

This work was financially supported by the Genetically Modified Organisms Breeding Major Projects of China (2014ZX08004), the National Natural Science Foundation of China (31301340), the Modern Agro-industry Technology Research System of China (CARS-004-PS10), the Program for Changjiang Scholars and Innovative Research Team in University, China (PCSIRT13073), the Jiangsu Collaborative Innovation Center for Modern Crop Production and an Open-end Fund by State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, China (ZW2013009).

Corresponding Authors:  XING Han, Tel/Fax: +86-25-84399526, E-mail:    
About author:  GUO Na, Mobile: +86-13913393081, E-mail:; XUE Dong, E-mail:;

Cite this article: 

GUO Na, XUE Dong, ZHANG Wei, ZHAO Jin-ming, XUE Chen-chen, YAN Qiang, XUE Jin-yan, WANG Hai-tang, ZHANG Yu-mei, XING Han. 2016. Overexpression of GmProT1 and GmProT2 increases tolerance to drought and salt stresses in transgenic Arabidopsis. Journal of Integrative Agriculture, 15(8): 1727-1743.

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