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| p53 and NFκB regulate microRNA-34c expression in porcine ovarian granulosa cells |
| XU Yuan1, ZHANG Ai-ling2, XIAO Guang1, ZHANG Zhe1, CHEN Zan-mou1, ZHANG Hao1, LI Jia-qi1 |
1 Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, P.R.China
2 College of Biological and Food Engineering, Guangdong University of Education, Guangzhou 510303, P.R.China |
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Abstract MicroRNAs (miRNAs) are endogenous 18–24 nucleotide (nt) non-coding RNAs, some of which have been indicated to play key roles in granulosa cells (GCs) function. However, little is known about how the miRNA gene expression itself is regulated in the GCs. Our previous study showed that miR-34c, identified to be a pro-apoptotic and anti-proliferative factor in many cell types, exerted the same effects in porcine GCs. Here, the transcriptional regulation of miR-34c expression in GCs was further investigated. 5´ rapid amplification of cDNA ends (RACE) assay indicated that the pri-miR-34c transcription start site was located in 1 556 bp upstream of pre-miR-34c. With dual-luciferase reporter assay, we confirmed a 69 bp core promoter region (–1 799 bp/–1 730 bp) was indispensable for the transcription of miR-34c. Chromatin immunoprecipitation (ChIP) assay demonstrated that p53, p50, and p65 could bind to the transcription factor binding sites within the 69 bp core promoter region. In addition, deletion of transcripition factor binding sites resulted in obvious change of the miR-34c promoter activity. Finally, using overexpression and knockdown of p53, p50, and p65 strategies, we showed that p53 and p50 could positively regulated miR-34c expression, whereas p65 neletively regulated miR-34c expression in GCs. Our results provide new data about the transcription regulatory mechanism of miRNA genes in GCs.
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Received: 30 June 2015
Accepted:
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| Fund: This work is supported by the National Natural Science Foundation of China (31201771) and the earmarked fund for the China Agriculture Research System (CARS-36). |
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Corresponding Authors:
LI Jia-qi, Tel: +86-20-85283519, Fax: +86-20-85280740,
E-mail: jqli@scau.edu.cn
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| About author: XU Yuan, E-mail: xuyuan0218@163.com; |
Cite this article:
XU Yuan, ZHANG Ai-ling, XIAO Guang, ZHANG Zhe, CHEN Zan-mou, ZHANG Hao, LI Jia-qi.
2016.
p53 and NFκB regulate microRNA-34c expression in porcine ovarian granulosa cells. Journal of Integrative Agriculture, 15(8): 1816-1824.
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