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| Development and evaluation of a herbal formulation with anti-pathogenic activities and probiotics stimulatory effects |
| ZHOU Qian1, 2, WANG Si-si1, YANG Guang1, ZHAO Wen1, 2, LI Hui-ling1 |
1 College of Food Science and Technology, Agricultural University of Hebei, Baoding 071001, P.R.China
2 Engineering Technology Research Center for Agricultural Product Processing of Hebei, Baoding 071001, P.R.China |
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Abstract Searching alternative feed antibiotics is always a study hotspot in the field of animal production. In this study, the anti-pathogenic activities and probiotics stimulatory effects of 30 kinds of herbs were screened through Oxford cup method and Hungate roll method. 15 herbs showed significantly antibacterial activities (P<0.05) against Escherichia coli, Salmonella enteritidis, Salmonella typhimurium and Staphylococcus aureus. Seven herbs showed greatly stimulatory promoting effects on Lactobacillus acidophilus and Bifidobacterium longum. Finally, five herbs were combined for the developed formulation with selective antibacterial properties and they were Mume Fructus (35%), Isatidis Folium (25%), Moslae Herba (20%), Chrysanthemi Indici Flos (13%) and Bupleuri Radix (7%). The herbal formulation showed significantly antibacterial abilities against four pathogens and stimulatory promoting abilities on two probiotics in vitro and the equivalent activities in broiler chickens in vivo against E. coli and L. acidophilus. The toxicity study showed it had no toxicity, which indicated that it would be a kind of preferred candidate for an alternative antibiotic in future animal production.
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Received: 24 April 2015
Accepted:
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| Fund: This study was supported by Hebei Science and Technology Support Program of China (13226602D). The authors are deeply grateful to the laboratory of Engineering Technology Research Center for Agricultural Product Processing of Hebei, China. |
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Corresponding Authors:
ZHAO Wen, Tel: +86-312-7528426, Mobile: +86-13582820221, Fax: +86-312-7528189, E-mail: 13582820221@163.com
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| About author: ZHOU Qian, E-mail: zhouqianyz513@126.com |
Cite this article:
ZHOU Qian, WANG Si-si, YANG Guang, ZHAO Wen, LI Hui-ling.
2016.
Development and evaluation of a herbal formulation with anti-pathogenic activities and probiotics stimulatory effects. Journal of Integrative Agriculture, 15(05): 1103-1111.
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