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| Effects of two efflux pump inhibitors on the drug susceptibility of Riemerella anatipestifer isolates from China |
| LI Ya-fei1, JIANG Hong-xia1, XIANG Rong2, SUN Na3, ZHANG Ya-nan1, ZHAO Li-qing1, GU Peng1, WANG Li-qiao1, ZENG Zhen-ling1 |
1 Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, P.R.China
2 Institute of Animal Healthy, Guangdong Academy of Agricultural Sciences/Guangdong Open Laboratory of Veterinary Public Health, Guangzhou 510640, P.R.China
3 Institute of Special Animal and Plant Sciences, Chinese Academic of Agricultural Sciences, Changchun 130112, P.R.China |
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摘要 The objective of this study was to verify the supposition that efflux might be involved in the drug resistance of Riemerella anatipestifer isolates. Two broad-spectrum efflux pump inhibitors, carbonyl cyanide 3-chlorophenylhydrazone (CCCP) and Phe-Arg-β-naphthylamide (PAβN), on the contribution of minimum inhibitory concentrations of amikacin, streptomycin, chloramphenicol, tetracycline, ceftriaxone, ceftazidime, nalidixic acid, levofloxacin, enrofloxacin, as well as ciprofloxacin against 69 clinical R. anatipestifer isolates were investigated. We first reported that the two efflux pump inhibitors could restore the antimicrobial susceptibility of R. anatipestifer isolates. It is suggested that active efflux system is possible to be linked with the development of resistance in R. anatipestifer isolates.
Abstract The objective of this study was to verify the supposition that efflux might be involved in the drug resistance of Riemerella anatipestifer isolates. Two broad-spectrum efflux pump inhibitors, carbonyl cyanide 3-chlorophenylhydrazone (CCCP) and Phe-Arg-β-naphthylamide (PAβN), on the contribution of minimum inhibitory concentrations of amikacin, streptomycin, chloramphenicol, tetracycline, ceftriaxone, ceftazidime, nalidixic acid, levofloxacin, enrofloxacin, as well as ciprofloxacin against 69 clinical R. anatipestifer isolates were investigated. We first reported that the two efflux pump inhibitors could restore the antimicrobial susceptibility of R. anatipestifer isolates. It is suggested that active efflux system is possible to be linked with the development of resistance in R. anatipestifer isolates.
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Received: 21 January 2015
Accepted:
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| Fund: This work was financially supported by the Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China (IRT13063) and the National Natural Science Foundation of China (31072169). |
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Corresponding Authors:
ZENG Zhen-ling, Tel: +86-20-85281204, Fax: +86-20-85284896, E-mail: zlzeng@scau.edu.cn
E-mail: zlzeng@scau.edu.cn
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| About author: LI Ya-fei, Mobile: +86-13422293073, E-mail: yafei.lee@163.com |
Cite this article:
LI Ya-fei, JIANG Hong-xia, XIANG Rong, SUN Na, ZHANG Ya-nan, ZHAO Li-qing, GU Peng, WANG Li-qiao, ZENG Zhen-ling .
2016.
Effects of two efflux pump inhibitors on the drug susceptibility of Riemerella anatipestifer isolates from China. Journal of Integrative Agriculture, 15(4): 929-933.
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