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Journal of Integrative Agriculture  2015, Vol. 14 Issue (3): 423-429    DOI: 10.1016/S2095-3119(14)60897-2
Special Focus: Development and Application of Plant Transformation Techniques Advanced Online Publication | Current Issue | Archive | Adv Search |
Development of lepidopteran pest-resistant transgenic japonica rice harboring a synthetic cry2A* gene
 JIN Yong-mei, MA Rui, YU Zhi-jing, WANG Ling, JIANG Wen-zhu, LIN Xiu-feng
1、Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun 130124, P.R.China
2、School of Life Science, Jilin Agricultural University, Changchun 130118, P.R.China
3、College of Plant Science, Jilin University, Changchun 130062, P.R.China
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摘要  A synthetic cry2A* gene encoding Bacillus thuringiensis (Bt) δ-endotoxin that resistance to lepidopteran pest was transformed into japonica rice variety Jijing 88, which is the most widely cultivated variety in Jilin Province, Northeast China, by Agrobacterium-mediated transformation. A total of 106 independent transformants overexpressing cry2A* gene driven by ubiquitin (Ubi) promoter was produced. Three single-copy homozygous transgenic lines were finally selected based on the results of PCR analysis, segregation ratio of Basta resistance, and Southern hybridization analyses. RT-PCR and enzyme linked immune sorbent assay (ELISA) revealed that cry2A* transcripts and protein were highly expressed in these lines. The high level of Cry2A* protein expression resulted in high resistance to rice striped stem borer as evidenced by insect feeding bioassays. Our results demonstrate that cry2A* transgenic japonica rice confers resistance to the rice striped stem borer in the laboratory conditions.

Abstract  A synthetic cry2A* gene encoding Bacillus thuringiensis (Bt) δ-endotoxin that resistance to lepidopteran pest was transformed into japonica rice variety Jijing 88, which is the most widely cultivated variety in Jilin Province, Northeast China, by Agrobacterium-mediated transformation. A total of 106 independent transformants overexpressing cry2A* gene driven by ubiquitin (Ubi) promoter was produced. Three single-copy homozygous transgenic lines were finally selected based on the results of PCR analysis, segregation ratio of Basta resistance, and Southern hybridization analyses. RT-PCR and enzyme linked immune sorbent assay (ELISA) revealed that cry2A* transcripts and protein were highly expressed in these lines. The high level of Cry2A* protein expression resulted in high resistance to rice striped stem borer as evidenced by insect feeding bioassays. Our results demonstrate that cry2A* transgenic japonica rice confers resistance to the rice striped stem borer in the laboratory conditions.
Keywords:  insect resistance       Bt gene       cry2A* transgenic rice       japonica rice variety       striped stem borer  
Received: 24 August 2014   Accepted:
Fund: 

This research was funded by the National Major Project for Transgenic Organism Breeding, China (201408001- 001-009).

Corresponding Authors:  LIN Xiu-feng, Tel/Fax: +86-431-87063078,E-mail: linxiufeng8581@163.com     E-mail:  linxiufeng8581@163.com
About author:  JIN Yong-mei, E-mail: ymjin0303@163.com;* These authors contributed equally to this study.

Cite this article: 

JIN Yong-mei, MA Rui, YU Zhi-jing, WANG Ling, JIANG Wen-zhu, LIN Xiu-feng. 2015. Development of lepidopteran pest-resistant transgenic japonica rice harboring a synthetic cry2A* gene. Journal of Integrative Agriculture, 14(3): 423-429.

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