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| Effects of Polysaccharides from Pulsatilla Decoction on the Microvascular Endothelial Glycocalyx |
| ZHANG Tao, WU Shuang, SUN Xiong, DUAN Hui-qin , MU Xiang |
| Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing 102206, P.R.China |
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摘要 Pulsatilla decoction is a famous traditional Chinese herbal formula for clearing heat and treating dysentery of animals or human. To elucidate its mechanism, many active components have been studied, however, the roles of its polysaccharides still remain unclear. This study aimed to explore effects of polysaccharides from Pulsatilla decoction (PPD) on the microvascular endothelial glycocalyx (eGC). The polysaccharides were extracted from PPD by water extraction and alcohol precipitation method. Mice were administered with PPD for 4 wk, and were then anesthetized with ether inhalation and were fixed by cardiac perfusion with gradient concentration alcian blue solution. The jejunum was sampled and jejunal mucosa was prepared for ultrathin sections by routine method and was analyzed by transmission electron microscope. The results indicated that the eGC was observed as a strong electron-dense smooth linear margin or nonuniform conglomerates coating cell membranes, and PPD significantly increased its thickness from (21.85±1.87) to (28.71±3.61) nm and improved its integrity. This study suggested that PPD may express their biological activities and protect against pathogenic factor damages by influencing the eGC.
Abstract Pulsatilla decoction is a famous traditional Chinese herbal formula for clearing heat and treating dysentery of animals or human. To elucidate its mechanism, many active components have been studied, however, the roles of its polysaccharides still remain unclear. This study aimed to explore effects of polysaccharides from Pulsatilla decoction (PPD) on the microvascular endothelial glycocalyx (eGC). The polysaccharides were extracted from PPD by water extraction and alcohol precipitation method. Mice were administered with PPD for 4 wk, and were then anesthetized with ether inhalation and were fixed by cardiac perfusion with gradient concentration alcian blue solution. The jejunum was sampled and jejunal mucosa was prepared for ultrathin sections by routine method and was analyzed by transmission electron microscope. The results indicated that the eGC was observed as a strong electron-dense smooth linear margin or nonuniform conglomerates coating cell membranes, and PPD significantly increased its thickness from (21.85±1.87) to (28.71±3.61) nm and improved its integrity. This study suggested that PPD may express their biological activities and protect against pathogenic factor damages by influencing the eGC.
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Received: 29 September 2013
Accepted:
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| Fund: This study was supported by grants from the National Natural Science Foundation of China (31001083), the Scientific Research Program of Beijing Municipal Commission of Education of China (KM201110020014, KZ201110020021) and the Beijing Natural Science Foundation, China (6142004). |
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Corresponding Authors:
MU Xiang, Fax/Tel: +86-10-80799515, E-mail: muxiang1109@sina.com
E-mail: muxiang1109@sina.com
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| About author: ZHANG Tao, Tel: +86-10-80793027, E-mail: zhangtao8008@126.com |
Cite this article:
ZHANG Tao, WU Shuang, SUN Xiong, DUAN Hui-qin , MU Xiang.
2014.
Effects of Polysaccharides from Pulsatilla Decoction on the Microvascular Endothelial Glycocalyx. Journal of Integrative Agriculture, 13(11): 2558-2561.
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