|
|
|
Effects of Fermentation Product Containing Phytase on Productive Performance, Egg Quality, and Phosphorous Apparent Metabolism of Laying Hens Fed Different Levels of Phosphorus |
WANG Zhi-hong, DONG Xiao-fang, TONG Jian-ming , XU Shang-zhong |
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China |
|
|
摘要 This study investigated the effects of fermentation product containing phytase (FPP) that was fermented using waste vinegar residue (WVR) as substrate from Aspergillus ficuum NTG-23 on productive performance, egg quality, and phosphorus apparent metabolism of laying hens. First, 375 22-wk-old Jinghong hens were allocated into 5 treatments (5 replicates of 15 hens each) in an 8-wk experiment for evaluating the parameters of productive performance, egg quality, serum, and tibia. Experimental diets contained 4% FPP and 96% corn-soybean diet. The levels of dicalcium phosphate (DCP) were 1.34, 1.01, 0.67, 0.34 and 0%. Next, thirty 31-wk-old Jinghong hens were fed 5 types of diets for evaluating phosphorous apparent metabolism rate. Egg productive rate, egg weight, feed conversion ratio, Haugh unit, egg albumen height, serum calcium, tibia ash, tibia ash calcium and tibia breaking strength were not different significantly among 5 treatments. The significant difference of average daily feed intake was not appeared when the DCP content of corn-soybean-FPP diet was reduced to 0.67%; the eggshell hardness, eggshell thickness and serum phosphorus were not reduced significantly until the DCP content of corn-soybean-FPP diet was reduced to 0.34%. The yolk color was improved when the laying hens fed deficient DCP corn-soybean-FPP diet. A 22.14% reduction in excreta phosphorus was observed when the laying hens fed low phosphorus (0.67% DCP) corn-soybean-FPP diet. A 30% elevation of phosphorus apparent metabolism rate was obtained when the DCP content of corn-soybean-FPP diet was decreased from 1.34 to 1.01%. The reducing cost of layer diet was totalized about 120 CNY 1 000 kg-1 diet when the content of DCP was 0.67% in corn-soybean-FPP diet. These results indicated that FPP could be applied in laying hen as a potential, cost-effective and rational application of WVR.
Abstract This study investigated the effects of fermentation product containing phytase (FPP) that was fermented using waste vinegar residue (WVR) as substrate from Aspergillus ficuum NTG-23 on productive performance, egg quality, and phosphorus apparent metabolism of laying hens. First, 375 22-wk-old Jinghong hens were allocated into 5 treatments (5 replicates of 15 hens each) in an 8-wk experiment for evaluating the parameters of productive performance, egg quality, serum, and tibia. Experimental diets contained 4% FPP and 96% corn-soybean diet. The levels of dicalcium phosphate (DCP) were 1.34, 1.01, 0.67, 0.34 and 0%. Next, thirty 31-wk-old Jinghong hens were fed 5 types of diets for evaluating phosphorous apparent metabolism rate. Egg productive rate, egg weight, feed conversion ratio, Haugh unit, egg albumen height, serum calcium, tibia ash, tibia ash calcium and tibia breaking strength were not different significantly among 5 treatments. The significant difference of average daily feed intake was not appeared when the DCP content of corn-soybean-FPP diet was reduced to 0.67%; the eggshell hardness, eggshell thickness and serum phosphorus were not reduced significantly until the DCP content of corn-soybean-FPP diet was reduced to 0.34%. The yolk color was improved when the laying hens fed deficient DCP corn-soybean-FPP diet. A 22.14% reduction in excreta phosphorus was observed when the laying hens fed low phosphorus (0.67% DCP) corn-soybean-FPP diet. A 30% elevation of phosphorus apparent metabolism rate was obtained when the DCP content of corn-soybean-FPP diet was decreased from 1.34 to 1.01%. The reducing cost of layer diet was totalized about 120 CNY 1 000 kg-1 diet when the content of DCP was 0.67% in corn-soybean-FPP diet. These results indicated that FPP could be applied in laying hen as a potential, cost-effective and rational application of WVR.
|
Received: 13 June 2013
Accepted:
|
Fund: This work was supported financially by a grant of the National Key Technologies R&D Program of China during the 12th Five-Year Plan period (2011BAD26B01-2) and an earmarked fund of the National System for Layer Production Technology of China (CARS-41-K16). |
Corresponding Authors:
DONG Xiao-fang, Tel: +86-10-62819257, E-mail:xiaofangd1124@sina.com
E-mail: xiaofangd1124@sina.com
|
About author: WANG Zhi-hong, Tel: +86-10-62819257, E-mail: wangzhihong0209@aliyun.com |
Cite this article:
WANG Zhi-hong, DONG Xiao-fang, TONG Jian-ming , XU Shang-zhong.
2014.
Effects of Fermentation Product Containing Phytase on Productive Performance, Egg Quality, and Phosphorous Apparent Metabolism of Laying Hens Fed Different Levels of Phosphorus. Journal of Integrative Agriculture, 13(10): 2253-2259.
|
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaAssociation of Official Analytical Chemists. 1990. OfficialMethods of Analysis. 15th ed. Association of OfficialAnalytical Chemists, Arlington, Virginia.Augspurger N R, Webel D M, Baker D H. 2007. AnEscherichia coli phytase expressed in yeast effectivelyreplaces inorganic phosphorus for finishing pigs and layinghens. Journal of Animal Science, 85, 1192-1198Bar A, Hurwitz S. 1984. Egg shell quality, medullary boneash, intestinal calcium and phosphorus absorption, andcalcium-binding protein in phosphorus-deficient hens.Poultry Science, 63, 1975-1979Carlos A B, Edwards H J. 1998. The effects of1,25-dihydroxycholecalciferol and phytase on the naturalphytate phosphorus utilization by laying hens. Poultry Science, 77, 850-858Casey A, Walsh G. 2004. Identification and characterizationof a phytase of potential commercial interest. Journal ofBiotechnology, 110, 313-322Englmaierová M, Dlouhá G, Marounek M, Sk?ivan M. 2012.Efficacy of contrast levels of non-phytate phosphorus andAspergillus niger phytase in hens fed wheat-maize-baseddiets. Czech Journal of Animal Science, 57, 499-505Gordon R W, Roland D S. 1997. Performance of commerciallaying hens fed various phosphorus levels, with and withoutsupplemental phytase. Poultry Science, 76, 1172-1177Haefner S, Knietsch A, Scholten E, Braun J, LohscheidtM, Zelder O. 2005. Biotechnological production andapplications of phytases. Applied Microbiology andBiotechnology, 68, 588-597Keshavarz K. 2003. The effect of different levels of nonphytatephosphorus with and without phytase on the performanceof four strains of laying hens. Poultry Science, 82, 71-91van der Klis J D, Versteegh H A, Simons P C, Kies A K. 1997.The efficacy of phytase in corn-soybean meal-based dietsfor laying hens. Poultry Science, 76, 1535-1542Kozlowski K, Jeroch H. 2011. Efficacy of different levels ofEscherichia coli phytase in hens fed maize-soybean mealbased diets with a decreased non-phytase phosphoruscontent. Journal of Animal and Feed Sciences, 20, 224-235Michael B C. 2001. Involvement of calcium and phosphorusin bone and shell quality of early maturing commerciallayers. World Poultry, 17, 16-19Miles R D, Costa P T, Harms R H. 1983. The influence ofdietary phosphorus level on laying hen performance,eggshell quality, and various blood parameters. PoultryScience, 62, 1033-1037Mitchell D B, Vogel K, Weimann B J, Pasamontes L, van LoonA P G M. 1997. The phytase subfamily of histidine acidphosphatases: Isolation of genes for two novel phytasesfrom the fungi Aspergillus terreus and Myceliophthorathermophila. Microbiology, 143, 245-252National Research Council. 1994. Nutrient Requirements ofPoultry. The National Academies Press, Washington, D.C.Payne R L, Lavergne T K, Southern L L. 2005. A comparisonof two sources of phytase in liquid and dry forms inbroilers. Poultry Science, 84, 265-272SAS Institute. 2000. SAS/STAT User’s Guide: Version 8. 1sted. SAS Institute, Cary, North Carolina.Simons P C, Versteegh H A, Jongbloed A W, Kemme P A,Slump P, Bos K D, Wolters M G, Beudeker R F, VerschoorG J. 1990. Improvement of phosphorus availability bymicrobial phytase in broilers and pigs. British Journal ofNutrition, 64, 525-540Song Z T, Dong X F, Tong J M. Wang Z H. 2013. In saccoevaluation of ruminal degradability of waste vinegarresidue as a feedstuff for ruminants. Animal ProductionScience, 53, 292-298Um J S, Paik I K. 1999. Effects of microbial phytasesupplementation on egg production, eggshell quality, andmineral retention of laying hens fed different levels ofphosphorus. Poultry Science, 78, 75-79Usayran N, Farran M T, Awadallah H H, Al-Hawi I R, AsmarR J, Ashkarian V M. 2001. Effects of added dietary fatand phosphorus on the performance and egg quality oflaying hens subjected to a constant high environmentaltemperature. Poultry Science, 80, 1695-1701Veum T L, Bollinger D W, Buff C E, Bedford M R. 2006. Agenetically engineered Escherichia coli phytase improvesnutrient utilization, growth performance, and bone strengthof young swine fed diets deficient in available phosphorus.Journal of Animal Science, 84, 1147-1158Vohra A, Satyanarayana T. 2003. Phytases: microbial sources,production, purification, and potential biotechnologicalapplications. Critical Reviews in Biotechnology, 23, 29-60Wang Z H, Dong X F, Tong J M, Wu Y Y, Zhang Q. 2010.Vinegar production residue as substrate for phytaseproduction by Aspergillus ficuum. Waste Management &Research, 28, 165-168Wang Z H, Dong X F, Tong J M, Zhang G Q, Zhang Q, XuS Z. 2011. Waste vinegar residue as substrate for phytaseproduction. Waste Management & Research, 29, 1262-1270Wu Y B, Ravindran V, Morel P C H, Hendriks W H, PierceJ. 2004. Evaluation of a microbial phytase, produced bysolid-state fermentation, in broiler diets. 1. influence onperformance, toe ash contents, and phosphorus equivalencyestimates. Journal of Applied Poultry Research, 13, 373-383Zhang G Q, Dong X F, Wang Z H, Tong J M, Zhang Q,Wang H X. 2010. Purification, character, and cloning of aphytase with low pH and strong proteolysis resistance fromAspergillus ficuum NTG-23 Bioresource Technology,101, 4125-4131. |
No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|