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Journal of Integrative Agriculture  2013, Vol. 12 Issue (6): 971-978    DOI: 10.1016/S2095-3119(13)60317-2
Crop Genetics · Breeding · Germplasm Resources Advanced Online Publication | Current Issue | Archive | Adv Search |
Statistic Analysis on Quantitative Characteristics for Developing the DUS Test Guideline of Ranunculus asiaticus L.
 LIU Yan-fang, ZHANG Jian-hua, LÜ Bo, YANG Xiao-hong, LI Yan-gang, WANG Ye, WANGJiang-min, ZHANG Hui, GUAN Jun-jiao
1 Institute of Quality Standard and Testing Technology Research, Yunnan Academy of Agricultural Sciences, Kunming 650205, P.R.China
2 Kunming DUS Test Station, Ministry of Agriculture, Kunming 650205, P.R.China
3 Division of Plant Variety Management, Seed Management Administration, Ministry of Agriculture, Beijing 100125, P.R.China
4 Faculty of Agriculture and Biological Sciences, Dali College, Dali 671000, P.R.China
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摘要  Selection of quantitative characteristics, division of their expression ranges, and selection of example varieties are key issues on developing DUS Test Guidelines, which are more crucial for quantitative characteristics since their expressions vary in different degrees. Taking the development of DUS Test Guideline of Ranunculus asiaticus L. as an example, this paper applied statistic-based approaches for the analyses of quantitative characteristics. We selected 9 quantitative characteristics from 18 pre-selected characteristics, based on within-variety uniformity, stability between different growing cycles, and correlation among characteristics, by the analyses of coefficient of variation, paired-samples t-test and partial correlation. The expression ranges of the 9 selected quantitative characteristics were divided into different states using descriptive statistics and distribution frequency of varieties. Eight of the 9 selected quantitative characteristics were categorized as standard characteristics as they showed one peak in distribution frequency of 120 varieties in various expressions of the characteristics, whereas, plant height can be categorized as grouping characteristic since it gave two peaks, and can group the varieties into pot and cut varieties. Finally, box-plot was applied to visually select the example varieties, and varieties 7, 12, and 28 were determined as the example varieties for plant height. The methods described in this paper are effective for the selection of quantitative characteristics, division of expression ranges, and selection of example varieties in Ranunculus asiaticus L. for DUS test, and may also be interest for other plant genera.

Abstract  Selection of quantitative characteristics, division of their expression ranges, and selection of example varieties are key issues on developing DUS Test Guidelines, which are more crucial for quantitative characteristics since their expressions vary in different degrees. Taking the development of DUS Test Guideline of Ranunculus asiaticus L. as an example, this paper applied statistic-based approaches for the analyses of quantitative characteristics. We selected 9 quantitative characteristics from 18 pre-selected characteristics, based on within-variety uniformity, stability between different growing cycles, and correlation among characteristics, by the analyses of coefficient of variation, paired-samples t-test and partial correlation. The expression ranges of the 9 selected quantitative characteristics were divided into different states using descriptive statistics and distribution frequency of varieties. Eight of the 9 selected quantitative characteristics were categorized as standard characteristics as they showed one peak in distribution frequency of 120 varieties in various expressions of the characteristics, whereas, plant height can be categorized as grouping characteristic since it gave two peaks, and can group the varieties into pot and cut varieties. Finally, box-plot was applied to visually select the example varieties, and varieties 7, 12, and 28 were determined as the example varieties for plant height. The methods described in this paper are effective for the selection of quantitative characteristics, division of expression ranges, and selection of example varieties in Ranunculus asiaticus L. for DUS test, and may also be interest for other plant genera.
Keywords:  DUS Test Guideline       quantitative characteristic       statistic analysis       expression range of characteristic       example variety  
Received: 06 August 2012   Accepted:
Fund: 

This work was supported by the Special Fund for Agroscientific Research in the Public Interest of Ministry of Agriculture, China (200903008-14).

Corresponding Authors:  Correspondence ZHANG Jian-hua, Tel/Fax: +86-871-65894691, E-mail: zhjhua6748@163.com     E-mail:  zhjhua6748@163.com
About author:  LIU Yan-fang, E-mail: liuyf528@163.com

Cite this article: 

LIU Yan-fang, ZHANG Jian-hua, Lü Bo, YANG Xiao-hong, LI Yan-gang, WANG Ye, WANGJiang-min , ZHANG Hui, , GUAN Jun-jiao. 2013. Statistic Analysis on Quantitative Characteristics for Developing the DUS Test Guideline of Ranunculus asiaticus L.. Journal of Integrative Agriculture, 12(6): 971-978.

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